Raw content of Bio::DB::SwissProt
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# $Id: SwissProt.pm,v 1.19 2002/12/01 00:05:19 jason Exp $
#
# BioPerl module for Bio::DB::SwissProt
#
# Cared for by Jason Stajich <jason@bioperl.org>
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
# Reworked to use Bio::DB::WebDBSeqI 2000-12-11
=head1 NAME
Bio::DB::SwissProt - Database object interface to SwissProt retrieval
=head1 SYNOPSIS
use Bio::DB::SwissProt;
$sp = new Bio::DB::SwissProt;
$seq = $sp->get_Seq_by_id('KPY1_ECOLI'); # SwissProt ID
# <4-letter-identifier>_<species 5-letter code>
# or ...
$seq = $sp->get_Seq_by_acc('P43780'); # SwissProt AC
# [OPQ]xxxxx
# In fact in this implementation
# these methods call the same webscript so you can use
# then interchangeably
# choose a different server to query
$sp = new Bio::DB::SwissProt('-servertype' => 'expasy',
'-hostlocation' => 'us');
$seq = $sp->get_Seq_by_id('BOLA_HAEIN'); # SwissProtID
=head1 DESCRIPTION
SwissProt is a curated database of proteins managed by the Swiss
Bioinformatics Institute. This is in contrast to EMBL/GenBank/DDBJ
which are archives of protein information. Additional tools for
parsing and manipulating swissprot files can be found at
ftp://ftp.ebi.ac.uk/pub/localsw/swissprot/Swissknife/.
Allows the dynamic retrieval of Sequence objects (Bio::Seq) from the
SwissProt database via an expasy retrieval. Perhaps through SRS
later.
In order to make changes transparent we have host type (currently only
expasy) and location (default to switzerland) separated out. This
allows the user to pick the closest expasy mirror for running their
queries.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bio.perl.org/MailList.html - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via email
or the web:
bioperl-bugs@bio.perl.org
http://bugzilla.bioperl.org/
=head1 AUTHOR - Jason Stajich
Email Jason Stajich E<lt>jason@bioperl.org E<lt>
Thanks go to Alexandre Gattiker E<lt>gattiker@isb-sib.chE<gt> of Swiss
Institute of Bioinformatics for helping point us in the direction of
the correct expasy scripts and for swissknife references.
Also thanks to Heikki Lehvaslaiho E<lt>heikki@ebi.ac.ukE<gt> for help with
adding EBI swall server.
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::DB::SwissProt;
use strict;
use vars qw(@ISA $MODVERSION %HOSTS $DEFAULTFORMAT $DEFAULTSERVERTYPE);
$MODVERSION = '0.8.1';
use HTTP::Request::Common;
use Bio::DB::WebDBSeqI;
@ISA = qw(Bio::DB::WebDBSeqI);
# global vars
$DEFAULTSERVERTYPE = 'ebi';
$DEFAULTFORMAT = 'swissprot';
# you can add your own here theoretically.
%HOSTS = (
'expasy' => {
'default' => 'us',
'baseurl' => 'http://%s/cgi-bin/sprot-retrieve-list.pl',
'hosts' =>
{
'switzerland' => 'ch.expasy.org',
'canada' => 'ca.expasy.org',
'china' => 'cn.expasy.org',
'taiwan' => 'tw.expasy.org',
'australia' => 'au.expasy.org',
'korea' => 'kr.expasy.org',
'us' => 'us.expasy.org',
},
# ick, CGI variables
'jointype' => ' ',
'idvar' => 'list',
'basevars' => [ ],
},
'ebi' => {
'default' => 'uk',
'baseurl' => 'http://%s/cgi-bin/dbfetch',
'hosts' => {
'uk' => 'www.ebi.ac.uk',
},
'jointype' => ',',
'idvar' => 'id',
'basevars' => [ 'db' => 'swall',
'style' => 'raw' ],
}
);
# new modules should be a little more lightweight and
# should use Bio::Root::Root
sub new {
my ($class, @args) = @_;
my $self = $class->SUPER::new(@args);
my ($format, $hostlocation,$servertype) =
$self->_rearrange([qw(FORMAT HOSTLOCATION SERVERTYPE)],
@args);
if( $format && $format !~ /(swiss)|(fasta)/i ) {
$self->warn("Requested Format $format is ignored because only SwissProt and Fasta formats are currently supported");
$format = $self->default_format;
}
$servertype = $DEFAULTSERVERTYPE unless $servertype;
$servertype = lc $servertype;
$self->servertype($servertype);
if ( $hostlocation ) {
$self->hostlocation(lc $hostlocation);
}
$self->request_format($format); # let's always override the format, as it must be swiss or fasta
return $self;
}
=head2 Routines from Bio::DB::RandomAccessI
=cut
=head2 get_Seq_by_id
Title : get_Seq_by_id
Usage : $seq = $db->get_Seq_by_id('ROA1_HUMAN')
Function: Gets a Bio::Seq object by its name
Returns : a Bio::Seq object
Args : the id (as a string) of a sequence
Throws : "id does not exist" exception
=cut
=head2 get_Seq_by_acc
Title : get_Seq_by_acc
Usage : $seq = $db->get_Seq_by_acc('X77802');
Function: Gets a Bio::Seq object by accession number
Returns : A Bio::Seq object
Args : accession number (as a string)
Throws : "acc does not exist" exception
=cut
=head2 get_Stream_by_id
Title : get_Stream_by_id
Usage : $stream = $db->get_Stream_by_id( [$uid1, $uid2] );
Function: Gets a series of Seq objects by unique identifiers
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of unique identifiers for
the desired sequence entries
=cut
=head2 get_Stream_by_acc
Title : get_Stream_by_acc
Usage : $seq = $db->get_Seq_by_acc([$acc1, $acc2]);
Function: Gets a series of Seq objects by accession numbers
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of accession numbers for
the desired sequence entries
Note : For GenBank, this just calls the same code for get_Stream_by_id()
=cut
=head2 get_Stream_by_batch
Title : get_Stream_by_batch
Usage : $seq = $db->get_Stream_by_batch($ref);
Function: Retrieves Seq objects from SwissProt 'en masse', rather than one
at a time. This is implemented the same way as get_Stream_by_id,
but is provided here in keeping with access methods of NCBI
modules.
Example :
Returns : a Bio::SeqIO stream object
Args : $ref : either an array reference, a filename, or a filehandle
from which to get the list of unique ids/accession numbers.
=cut
sub get_Stream_by_batch {
my ($self, $ids) = @_;
return $self->get_Stream_by_id( $ids);
}
=head2 Implemented Routines from Bio::DB::WebDBSeqI interface
=cut
=head2 get_request
Title : get_request
Usage : my $url = $self->get_request
Function: returns a HTTP::Request object
Returns :
Args : %qualifiers = a hash of qualifiers (ids, format, etc)
=cut
sub get_request {
my ($self, @qualifiers) = @_;
my ($uids, $format) = $self->_rearrange([qw(UIDS FORMAT)],
@qualifiers);
if( !defined $uids ) {
$self->throw("Must specify a value for uids to query");
}
my ($f,undef) = $self->request_format($format);
my %vars = (
@{$HOSTS{$self->servertype}->{'basevars'}},
( 'format' => $f )
);
my $url = $self->location_url;
my $uid;
my $jointype = $HOSTS{$self->servertype}->{'jointype'} || ' ';
my $idvar = $HOSTS{$self->servertype}->{'idvar'} || 'id';
if( ref($uids) =~ /ARRAY/i ) {
# HTTP::Request automagically converts the ' ' to %20
$uid = join($jointype, @$uids);
} else {
$uid = $uids;
}
$vars{$idvar} = $uid;
return POST $url, \%vars;
}
=head2 postprocess_data
Title : postprocess_data
Usage : $self->postprocess_data ( 'type' => 'string',
'location' => \$datastr);
Function: process downloaded data before loading into a Bio::SeqIO
Returns : void
Args : hash with two keys - 'type' can be 'string' or 'file'
- 'location' either file location or string
reference containing data
=cut
# don't need to do anything
sub postprocess_data {
my ($self, %args) = @_;
return;
}
=head2 default_format
Title : default_format
Usage : my $format = $self->default_format
Function: Returns default sequence format for this module
Returns : string
Args : none
=cut
sub default_format {
return $DEFAULTFORMAT;
}
=head2 Bio::DB::SwissProt specific routines
=cut
=head2 servertype
Title : servertype
Usage : my $servertype = $self->servertype
$self->servertype($servertype);
Function: Get/Set server type
Returns : string
Args : server type string [optional]
=cut
sub servertype {
my ($self, $servertype) = @_;
if( defined $servertype && $servertype ne '') {
$self->throw("You gave an invalid server type ($servertype)".
" - available types are ".
keys %HOSTS) unless( $HOSTS{$servertype} );
$self->{'_servertype'} = $servertype;
$self->{'_hostlocation'} = $HOSTS{$servertype}->{'default'};
# make sure format is reset properly in that different
# servers have different syntaxes
my ($existingformat,$seqioformat) = $self->request_format;
$self->request_format($existingformat);
}
return $self->{'_servertype'} || $DEFAULTSERVERTYPE;
}
=head2 hostlocation
Title : hostlocation
Usage : my $location = $self->hostlocation()
$self->hostlocation($location)
Function: Set/Get Hostlocation
Returns : string representing hostlocation
Args : string specifying hostlocation [optional]
=cut
sub hostlocation {
my ($self, $location ) = @_;
$location = lc $location;
my $servertype = $self->servertype;
$self->throw("Must have a valid servertype defined not $servertype")
unless defined $servertype;
my %hosts = %{$HOSTS{$servertype}->{'hosts'}};
if( defined $location && $location ne '' ) {
if( ! $hosts{$location} ) {
$self->throw("Must specify a known host, not $location,".
" possible values (".
join(",", sort keys %hosts ). ")");
}
$self->{'_hostlocation'} = $location;
}
return $self->{'_hostlocation'};
}
=head2 location_url
Title : location
Usage : my $url = $self->location_url()
Function: Get host url
Returns : string representing url
Args : none
=cut
sub location_url {
my ($self) = @_;
my $servertype = $self->servertype();
my $location = $self->hostlocation();
if( ! defined $location || !defined $servertype ) {
$self->throw("must have a valid hostlocation and servertype set before calling location_url");
}
return sprintf($HOSTS{$servertype}->{'baseurl'},
$HOSTS{$servertype}->{'hosts'}->{$location});
}
=head2 request_format
Title : request_format
Usage : my ($req_format, $ioformat) = $self->request_format;
$self->request_format("genbank");
$self->request_format("fasta");
Function: Get/Set sequence format retrieval. The get-form will normally not
be used outside of this and derived modules.
Returns : Array of two strings, the first representing the format for
retrieval, and the second specifying the corresponding SeqIO format.
Args : $format = sequence format
=cut
sub request_format {
my ($self, $value) = @_;
if( defined $value ) {
if( $self->servertype =~ /expasy/ ) {
if( $value =~ /sprot/ || $value =~ /swiss/ ) {
$self->{'_format'} = [ 'sprot', 'swiss'];
} elsif( $value =~ /^fa/ ) {
$self->{'_format'} = [ 'fasta', 'fasta'];
} else {
$self->warn("Unrecognized format $value requested");
$self->{'_format'} = [ 'fasta', 'fasta'];
}
} elsif( $self->servertype =~ /ebi/ ) {
if( $value =~ /sprot/ || $value =~ /swiss/ ) {
$self->{'_format'} = [ 'swissprot', 'swiss' ];
} elsif( $value =~ /^fa/ ) {
$self->{'_format'} = [ 'fasta', 'fasta'];
} else {
$self->warn("Unrecognized format $value requested");
$self->{'_format'} = [ 'swissprot', 'swiss'];
}
}
}
return @{$self->{'_format'}};
}
1;
__END__
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