Raw content of Bio::SeqIO::qual
<![CDATA[# $Id: qual.pm,v 1.22 2002/12/27 19:42:32 birney Exp $
#
# Copyright (c) 1997-9 bioperl, Chad Matsalla. All Rights Reserved.
# This module is free software; you can redistribute it and/or
# modify it under the same terms as Perl itself.
#
# Copyright Chad Matsalla
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::SeqIO::Qual - .qual file input/output stream
=head1 SYNOPSIS
Do not use this module directly. Use it via the Bio::SeqIO class
(see L<Bio::SeqIO> for details).
=head1 DESCRIPTION
This object can transform .qual (similar to fasta) objects to and from
Bio::Seq::SeqWithQuality objects. See L<Bio::Seq::SeqWithQuality> for
details.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://www.bioperl.org/MailList.shtml - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via email
or the web:
bioperl-bugs@bio.perl.org
http://bugzilla.bioperl.org/
=head1 AUTHOR Chad Matsalla
Chad Matsalla
bioinformatics@dieselwurks.com
=head1 CONTRIBUTORS
Jason Stajich, jason@bioperl.org
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::SeqIO::qual;
use vars qw(@ISA);
use strict;
use Bio::SeqIO;
use Bio::Seq::SeqFactory;
require 'dumpvar.pl';
@ISA = qw(Bio::SeqIO);
sub _initialize {
my($self,@args) = @_;
$self->SUPER::_initialize(@args);
if( ! defined $self->sequence_factory ) {
$self->sequence_factory(new Bio::Seq::SeqFactory
(-verbose => $self->verbose(),
-type => 'Bio::Seq::PrimaryQual'));
}
}
=head2 next_seq()
Title : next_seq()
Usage : $scf = $stream->next_seq()
Function: returns the next scf sequence in the stream
Returns : Bio::Seq::PrimaryQual object
Notes : Get the next quality sequence from the stream.
=cut
sub next_seq {
my ($self,@args) = @_;
my ($qual,$seq);
my $alphabet;
local $/ = "\n>";
return unless my $entry = $self->_readline;
if ($entry eq '>') { # very first one
return unless $entry = $self->_readline;
}
# original: my ($top,$sequence) = $entry =~ /^(.+?)\n([^>]*)/s
my ($top,$sequence) = $entry =~ /^(.+?)\n([^>]*)/s
or $self->throw("Can't parse entry [$entry]");
my ($id,$fulldesc) = $top =~ /^\s*(\S+)\s*(.*)/
or $self->throw("Can't parse fasta header");
$id =~ s/^>//;
# create the seq object
$sequence =~ s/\n+/ /g;
return $self->sequence_factory->create
(-qual => $sequence,
-id => $id,
-primary_id => $id,
-display_id => $id,
-desc => $fulldesc
);
}
=head2 _next_qual
Title : _next_qual
Usage : $seq = $stream->_next_qual() (but do not do
that. Use $stream->next_seq() instead)
Function: returns the next quality in the stream
Returns : Bio::Seq::PrimaryQual object
Args : NONE
Notes : An internal method. Gets the next quality in
the stream.
=cut
sub _next_qual {
my $qual = next_primary_qual( $_[0], 1 );
return $qual;
}
=head2 next_primary_qual()
Title : next_primary_qual()
Usage : $seq = $stream->next_primary_qual()
Function: returns the next sequence in the stream
Returns : Bio::PrimaryQual object
Args : NONE
=cut
sub next_primary_qual {
# print("CSM next_primary_qual!\n");
my( $self, $as_next_qual ) = @_;
my ($qual,$seq);
local $/ = "\n>";
return unless my $entry = $self->_readline;
if ($entry eq '>') { # very first one
return unless $entry = $self->_readline;
}
my ($top,$sequence) = $entry =~ /^(.+?)\n([^>]*)/s
or $self->throw("Can't parse entry [$entry]");
my ($id,$fulldesc) = $top =~ /^\s*(\S+)\s*(.*)/
or $self->throw("Can't parse fasta header");
$id =~ s/^>//;
# create the seq object
$sequence =~ s/\n+/ /g;
if ($as_next_qual) {
$qual = Bio::Seq::PrimaryQual->new(-qual => $sequence,
-id => $id,
-primary_id => $id,
-display_id => $id,
-desc => $fulldesc
);
}
return $qual;
}
=head2 write_seq
Title : write_seq(-source => $source, -header => "some information")
Usage : $obj->write_seq( -source => $source,
-header => "some information");
Function: Write out an list of quality values to a fasta-style file.
Returns : Nothing.
Args : Requires: a reference to a SeqWithQuality object or a
PrimaryQual object as the -source. Optional: information
for the header.
Notes : If no -header is provided, $obj->id() will be used where
$obj is a reference to either a SeqWithQuality object or a
PrimaryQual object. If $source->id() fails, ">unknown" will be
the header. If the SeqWithQuality object has $source->length() of
"DIFFERENT" (read the pod, luke), write_seq will use the length
of the PrimaryQual object within the SeqWithQuality object.
=cut
sub write_seq {
my ($self,@args) = @_;
my ($source) = $self->_rearrange([qw(SOURCE)], @args);
if (!$source || ( !$source->isa('Bio::Seq::SeqWithQuality') &&
!$source->isa('Bio::Seq::PrimaryQual') )) {
$self->throw("You must pass a Bio::Seq::SeqWithQuality or a Bio::Seq::PrimaryQual object to write_seq as a parameter named \"source\"");
}
my $header = $source->id();
if (!$header) { $header = "unknown"; }
my @quals = $source->qual();
# ::dumpValue(\@quals);
$self->_print (">$header \n");
my (@slice,$max,$length);
$length = $source->length();
if ($length eq "DIFFERENT") {
$self->warn("You passed a SeqWithQuality object that contains a sequence and quality of differing lengths. Using the length of the PrimaryQual component of the SeqWithQuality object.");
$length = $source->qual_obj()->length();
}
# print("Printing $header to a file.\n");
for (my $count = 1; $count<=$length; $count+= 50) {
if ($count+50 > $length) { $max = $length; }
else { $max = $count+49; }
my @slice = @{$source->subqual($count,$max)};
$self->_print (join(' ',@slice), "\n");
}
$self->flush if $self->_flush_on_write && defined $self->_fh;
return 1;
}
1;
__END__
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