Raw content of Bio::Tools::Phylo::Molphy # $Id: Molphy.pm,v 1.3 2002/10/22 07:45:23 lapp Exp $ # # BioPerl module for Bio::Tools::Phylo::Molphy # # Cared for by Jason Stajich <jason@bioperl.org> # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Tools::Phylo::Molphy - DESCRIPTION of Object =head1 SYNOPSIS use Bio::Tools::Phylo::Molphy; my $parser = new Bio::Tools::Phylo::Molphy(-file => 'output.protml'); while( my $result = $parser->next_result ) { } =head1 DESCRIPTION A parser for Molphy output (protml,dnaml) =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/MailList.shtml - About the mailing lists =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web: bioperl-bugs@bioperl.org http://bugzilla.bioperl.org/ =head1 AUTHOR - Jason Stajich Email jason@bioperl.org Describe contact details here =head1 CONTRIBUTORS Additional contributors names and emails here =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Tools::Phylo::Molphy; use vars qw(@ISA); use strict; use Bio::Tools::Phylo::Molphy::Result; use Bio::Root::Root; use Bio::Root::IO; use Bio::TreeIO; use IO::String; @ISA = qw(Bio::Root::Root Bio::Root::IO ); =head2 new Title : new Usage : my $obj = new Bio::Tools::Phylo::Molphy(); Function: Builds a new Bio::Tools::Phylo::Molphy object Returns : Bio::Tools::Phylo::Molphy Args : -fh/-file => $val, # for initing input, see Bio::Root::IO =cut sub new { my($class,@args) = @_; my $self = $class->SUPER::new(@args); $self->_initialize_io(@args); return $self; } =head2 next_result Title : next_result Usage : my $r = $molphy->next_result Function: Get the next result set from parser data Returns : Bio::Tools::Phylo::Molphy::Result object Args : none =cut sub next_result{ my ($self) = @_; # A little statemachine for the parser here my ($state,$transition_ct, @transition_matrix, %transition_mat, @resloc,) = ( 0,0); my ( %subst_matrix, @treelines, @treedata, %frequencies); my ( $treenum,$possible_trees, $model); while( defined ( $_ = $self->_readline()) ) { if( /^Relative Substitution Rate Matrix/ ) { if( %subst_matrix ) { $self->_pushback($_); last; } $state = 0; my ( @tempdata); @resloc = (); while( defined ($_ = $self->_readline) ) { last if (/^\s+$/); # remove leading/trailing spaces s/^\s+//; s/\s+$//; my @data = split; my $i = 0; for my $l ( @data ) { if( $l =~ /\D+/ ) { push @resloc, $l; } $i++; } push @tempdata, \@data; } my $i = 0; for my $row ( @tempdata ) { my $j = 0; for my $col ( @$row ) { if( $i == $j ) { # empty string for diagonals $subst_matrix{$resloc[$i]}->{$resloc[$j]} = ''; } else { $subst_matrix{$resloc[$i]}->{$resloc[$j]} = $col; } $j++; } $i++; } } elsif( /^Transition Probability Matrix/ ) { if( /1\.0e7/ ) { $state = 1; $transition_ct = 0; } else { $state = 0; } } elsif ( /Acid Frequencies/ ) { $state = 0; $self->_readline(); # skip the next line while( defined( $_ = $self->_readline) ) { unless( /^\s+/) { $self->_pushback($_); last; } s/^\s+//; s/\s+$//; my ($index,$res,$model,$data) = split; $frequencies{$res} = [ $model,$data]; } } elsif( /^(\d+)\s*\/\s*(\d+)\s+(.+)\s+model/ ) { my @save = ($1,$2,$3); # finish processing the transition_matrix my $i =0; foreach my $row ( @transition_matrix ) { my $j = 0; foreach my $col ( @$row ) { $transition_mat{$resloc[$i]}->{$resloc[$j]} = $col; $j++; } $i++; } if( defined $treenum ) { $self->_pushback($_); last; } $state = 2; ($treenum,$possible_trees, $model) = @save; $model =~ s/\s+/ /g; } elsif( $state == 1 ) { next if( /^\s+$/ ); s/^\s+//; s/\s+$//; # because the matrix is split up into 2-10 column sets push @{$transition_matrix[$transition_ct++]}, split ; $transition_ct = 0 if $transition_ct % 20 == 0; } elsif( $state == 2 ) { if( s/^(\d+)\s+(\-?\d+(\.\d+)?)\s+// ) { push @treedata, [ $1,$2]; } # save this for the end so that we can # be efficient and only open one tree parser push @treelines, $_; } } # waiting till the end to do this, is it better my @trees; if( @treelines ) { my $strdat = IO::String->new(join('',@treelines)); my $treeio = new Bio::TreeIO(-fh => $strdat, -format => 'newick'); while( my $tree = $treeio->next_tree ) { if( @treedata ) { my $dat = shift @treedata; # set the associated information $tree->id($dat->[0]); $tree->score($dat->[1]); } push @trees, $tree; } } my $result = new Bio::Tools::Phylo::Molphy::Result (-trees => \@trees, -substitution_matrix => \%subst_matrix, -transition_matrix => \%transition_mat, -frequencies => \%frequencies, -model => $model, -search_space => $possible_trees, ); } 1;