Raw content of Bio::Tools::Tmhmm # $Id: Tmhmm.pm,v 1.6 2002/10/22 07:45:22 lapp Exp $ # # BioPerl module for Bio::Tools::Tmhmm # # Copyright Balamurugan Kumarasamy # # You may distribute this module under the same terms as perl itself # # POD documentation - main docs before the code # # Copyright # # You may distribute this module under the same terms as perl itself =head1 NAME Bio::Tools::Tmhmm - parse TmHMM output (transmembrane HMM) =head1 SYNOPSIS use Bio::Tools::Tmhmm; my $parser = new Bio::Tools::Tmhmm(-fh =>$filehandle ); while( my $tmhmm_feat = $parser->next_result ) { #do something #eg push @tmhmm_feat, $tmhmm_feat; } =head1 DESCRIPTION Parser for Tmhmm output =head1 FEEDBACK =head2 Mailing Lists user feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/MailList.shtml - About the mailing lists =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web: bioperl-bugs@bioperl.org http://bugzilla.bioperl.org/ =head1 AUTHOR - Bala Email savikalpa@fugu-sg.org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut package Bio::Tools::Tmhmm; use vars qw(@ISA); use strict; use Bio::Tools::AnalysisResult; use Bio::Root::Root; use Bio::SeqFeature::FeaturePair; use Bio::Root::IO; use Bio::SeqFeature::Generic; @ISA = qw(Bio::Root::Root Bio::Root::IO Bio::Tools::AnalysisResult); =head2 new Title : new Usage : my $obj = new Bio::Tools::Tmhmm(); Function: Builds a new Bio::Tools::Tmhmm object Returns : Bio::Tools::Tmhmm Args : -fh/-file => $val, # for initing input, see Bio::Root::IO =cut sub new { my($class,@args) = @_; my $self = $class->SUPER::new(@args); $self->_initialize_io(@args); return $self; } =head2 next_result Title : next_result Usage : my $feat = $Tmhmm->next_result Function: Get the next result set from parser data Returns : Bio::SeqFeature::Generic Args : none =cut sub next_result { my ($self) = @_; my $line; # parse my $id; while ($_=$self->_readline()) { $line = $_; chomp $line; next if /^$/; if ($line=~/^#\s+(\S+)/) { #if the line starts with a '#' for example in # 13 Length: 522 #assign 13 as the id. $id = $1; my ($junk, $values) = split /:/; $self->_seqname($id); next; } elsif ($line=~/^(\S+)\s+(\S+)\s+(\w+)\s+(\d+)\s+(\d+)/) { # Example :- 13 TMHMM2.0 inside 1 120 # assign $orien(inside) $start(1) and $end(120) my $orien = $3; my $start = $4; my $end = $5; $orien = uc ($orien); if ($orien eq "TMHELIX") { my (%feature); $feature{name} = $self->_seqname; $feature{start} = $start; $feature{end} = $end; $feature{source} ='tmhmm'; $feature{primary}= 'transmembrane'; $feature{program} ='tmhmm'; $feature{logic_name} = 'TMHelix'; my $new_feat= $self->create_feature(\%feature); return $new_feat; } next; } next; } } =head2 create_feature Title : create_feature Usage : obj->create_feature(\%feature) Function: Internal(not to be used directly) Returns : A Bio::SeqFeature::Generic object Args : =cut sub create_feature { my ($self, $feat) = @_; # create feature object my $feature = Bio::SeqFeature::Generic->new(-seq_id => $feat->{name}, -start => $feat->{start}, -end => $feat->{end}, -score => $feat->{score}, -source => $feat->{source}, -primary => $feat->{primary}, -logic_name => $feat->{logic_name}, ); return $feature; } =head2 _seqname Title : _seqname Usage : obj->_seqname($seqname) Function: Internal(not to be used directly) Returns : Args : seqname =cut sub _seqname{ my ($self,$seqname)=@_; if (defined $seqname){ $self->{'seqname'}=$seqname; } return $self->{'seqname'}; } 1;