Bio::EnsEMBL::Funcgen::Parsers cisred
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Included modules
Bio::EnsEMBL::DBEntry
Bio::EnsEMBL::Funcgen::ExternalFeature
Bio::EnsEMBL::Funcgen::Parsers::BaseExternalParser
Bio::EnsEMBL::Utils::Exception qw ( throw )
File::Basename
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Bio::EnsEMBL::Funcgen::Parsers::BaseExternalParser
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new
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parse_and_load
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Methods description
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Methods code
newdescriptionprevnextTop
sub new {
  my $caller = shift;
  my $class = ref($caller) || $caller;

  my $self = $class->SUPER::new(@_);

  #Set default feature_type and feature_set config
$self->{'feature_types'} = { 'cisRED Search Region' => { name => 'cisRED Search Region', class => 'Region', description => 'cisRED search region', }, 'cisRED Motif' => { name => 'cisRED Motif', class => 'Regulatory Motif', description => 'cisRED atomic motif', }, }; $self->{feature_sets} = { 'cisRED search regions' => { feature_type =>\$ self->{'feature_types'}{'cisRED Search Region'}, display_label => 'cisRED searches', analysis => { -logic_name => 'cisRED', -description => 'cisRED motif search (www.cisred.org)', -display_label => 'cisRED', -displayable => 1, }, xrefs => 1, }, 'cisRED motifs' => { feature_type =>\$ self->{'feature_types'}{'cisRED Motif'}, #display_label => 'cisRED motifs',#defaults to name
analysis => { -logic_name => 'cisRED', -description => 'cisRED motif search (www.cisred.org)', -display_label => 'cisRED', -displayable => 1, }, xrefs => 1, }, }; $self->validate_and_store_feature_types; $self->set_feature_sets; return $self; } # Parse file and return hashref containing:
#
# - arrayref of features
# - arrayref of factors
#To do
# 1 This needs to take both file names, motifs, then search regions. Like the Bed/GFF importers do.
}
parse_and_loaddescriptionprevnextTop
sub parse_and_load {
  my ($self, $files, $old_assembly, $new_assembly) = @_;
  $self->log_header("Parsing cisRED data");

  my $analysis_adaptor = $self->db->get_AnalysisAdaptor();
  #my %features_by_group; # name -> factor_id
my %groups; my %slice_cache; my $extf_adaptor = $self->db->get_ExternalFeatureAdaptor; my $dbentry_adaptor = $self->db->get_DBEntryAdaptor; my $ftype_adaptor = $self->db->get_FeatureTypeAdaptor; #my $display_name_cache = $self->build_display_name_cache('gene');
# this object is only used for projection
my $dummy_analysis = new Bio::EnsEMBL::Analysis(-logic_name => 'CisREDProjection'); # ----------------------------------------
# We need a "blank" factor for those features which aren't assigned factors
# Done this way to maintain referential integrity
#my $blank_factor_id = $self->get_blank_factor_id($db_adaptor);
#More validation of files here?
my ($motif_file) = grep(/motif/, @$files); my ($search_file) = grep(/search/, @$files); my $species = $self->db->species; if(! $species){ throw('Must define a species to define the external_db'); } #Just to make sure we hav homo_sapiens and not Homo Sapiens
($species = lc($species)) =~ s/ /_/; # Parse motifs.txt file
$self->log_header("Parsing cisRED motifs from $motif_file"); my $skipped = 0; my $skipped_xref = 0; #my $coords_changed = 0;
my $cnt = 0; open (FILE, "<$motif_file") || die "Can't open $motif_file"; <FILE>; # skip header
while (<FILE>) { next if ($_ =~ /^\s*\#/o || $_ =~ /^\s*$/o); chomp; #name chromosome start end strand group_name ensembl_gene
#craHsap1 1 168129978 168129997 - crtHsap40066,crtHsap40060 ENSG00000000457
#craHsap2 1 168129772 168129781 - crtHsap40068,crtHsap40193,crtHsap40130 ENSG00000000457
#So we only ever have one atomic motif, which may belong to several groups
#Do not store atmoic motifs as feature types as this is the actual feature
#simply use the feature_set->feature_type and store the atmoic motif id as the name
my ($motif_name, $chromosome, $start, $end, $strand, $groups, $gene_id) = split/\t/o; #($gene_id) = $gene_id =~ /(ENS.*G\d{11})/;
my @group_names = split/,/, $groups; #These are stranded features, so either - or +, never 0;
$strand = ($strand eq '-') ? -1 : 1; if(! exists $slice_cache{$chromosome}){ if($old_assembly){ $slice_cache{$chromosome} = $self->slice_adaptor->fetch_by_region('chromosome', $chromosome, undef, undef, undef, $old_assembly); }else{ $slice_cache{$chromosome} = $self->slice_adaptor->fetch_by_region('chromosome', $chromosome); } } if(! defined $slice_cache{$chromosome}){ warn "Can't get slice $chromosome for motif $motif_name\n"; $skipped++; next; } #get feature_type first
#we are not maintaining this link in the DB!
#Do we need another xref for this or a different table?
#if ($group_name && $group_name ne '' && $group_name !~ /\s/o) {
#
# if(! exists $features_by_group{$group_name}){
# $features_by_group{$group_name} = $ftype_adaptor->fetch_by_name('crtHsap'.$group_name);
#
# if(! defined $features_by_group{$group_name}){
# ($features_by_group{$group_name}) = @{$ftype_adaptor->store(Bio::EnsEMBL::Funcgen::FeatureType->new
# (
# -name => 'crtHsap'.$group_name,
# -class => 'Regulatory Motif',
# -description => 'cisRED group',
# ))};
# }
# }
# }
#}else{
# throw("Found cisRED feature $motif_name with no group_name, unable to defined feature_type");
#}
foreach my $group(@group_names){ next if exists $groups{$group}; #else store the new group as a feature_type and set $group to be the feature_type
($group) = @{$ftype_adaptor->store(Bio::EnsEMBL::Funcgen::FeatureType->new ( -name => $group, -class => 'Regulatory Motif', -description => 'cisRED group', ))}; } #my $ftype = (defined $features_by_group{$group_name}) ? $features_by_group{$group_name} : $self->{'feature_sets'}{'cisRED group motifs'}->feature_type;
my $feature = Bio::EnsEMBL::Funcgen::ExternalFeature->new ( -display_label => $motif_name, -start => $start, -end => $end, -strand => $strand, #-feature_type => $ftype,
-associated_feature_types =>\@ group_names, -feature_set => $self->{'feature_sets'}{'cisRED motifs'}, -slice => $slice_cache{$chromosome}, ); # project if necessary
if ($new_assembly) { $feature = $self->project_feature($feature, $new_assembly); if(! defined $feature){ $skipped ++; next; } #$coords_changed++ if ($projected_feature->start() != $start || $projected_feature->end() != $end);
} ($feature) = @{$extf_adaptor->store($feature)}; $cnt++; #need to validate gene_id here
#retrieve from db?
#could have version here if we use the correct dnadb to build the cache
#Build Xref here
if (! $gene_id) { warn("No xref available for motif $motif_name\n"); $skipped_xref++; next; } my $display_name = $self->get_core_display_name_by_stable_id($self->db->dnadb, $gene_id, 'gene'); #Handle release/version in xref version as stable_id version?
my $dbentry = Bio::EnsEMBL::DBEntry->new( -dbname => $species.'_core_Gene', #-release => $self->db->dnadb->dbc->dbname,
-status => 'KNOWNXREF', #-display_label_linkable => 1,
-#db_display_name => $self->db->dnadb->dbc->dbname,
-db_display_name => 'EnsemblGene', -type => 'MISC',#this is external_db.type
-primary_id => $gene_id, -display_id => $display_name, -info_type => 'MISC', -info_text => 'GENE', -linkage_annotation => 'cisRED motif gene', #could have version here if we use the correct dnadb to build the cache
); $dbentry_adaptor->store($dbentry, $feature->dbID, 'ExternalFeature', 1);#1 is ignore release flag
} close FILE; $self->log("Stored $cnt cisRED ExternalFeature motif"); $self->log("Skipped $skipped cisRED ExternalFeature motif imports"); $self->log("Skipped an additional $skipped_xref DBEntry imports"); # ----------------------------------------
# Search regions
# read search_regions.txt from same location as $file
#my $search_regions_file = dirname($file) . "/search_regions.txt";
#my $search_file;
#($search_regions_file = $file) =~ s/motifs/searchregions/;
$skipped = 0; $cnt = 0; $skipped_xref = 0; $self->log_header("Parsing cisRED search regions from $search_file"); open (SEARCH_REGIONS, "<$search_file") || die "Can't open $search_file"; <SEARCH_REGIONS>; # skip header
while (<SEARCH_REGIONS>) { chomp; my ($id, $chromosome, $start, $end, $strand, $gene_id) = split; my $display_id = $self->get_core_display_name_by_stable_id($self->db->dnadb, $gene_id, 'gene'); my $name = "CisRed_Search_$id"; if(! exists $slice_cache{$chromosome}){ if($old_assembly){ $slice_cache{$chromosome} = $self->slice_adaptor->fetch_by_region('chromosome', $chromosome, undef, undef, undef, $old_assembly); }else{ $slice_cache{$chromosome} = $self->slice_adaptor->fetch_by_region('chromosome', $chromosome); } } if(! defined $slice_cache{$chromosome}){ warn "Can't get slice $chromosome for search region $name\n"; next; } my $search_feature = Bio::EnsEMBL::Funcgen::ExternalFeature->new ( -display_label => $name, -start => $start, -end => $end, -strand => $strand, -feature_type => $self->{'feature_sets'}{'cisRED search regions'}->feature_type, -feature_set => $self->{'feature_sets'}{'cisRED search regions'}, -slice => $slice_cache{$chromosome}, ); # project if necessary
if ($new_assembly) { $search_feature = $self->project_feature($search_feature); if(! defined $search_feature){ $skipped ++; next; } } $extf_adaptor->store($search_feature); $cnt++; #Build Xref here
#need to validate gene_id here!!
if (! $gene_id) { warn("Can't get internal ID for $gene_id\n"); $skipped_xref++; next; } my $display_name = $self->get_core_display_name_by_stable_id($self->db->dnadb, $gene_id, 'gene'); my $dbentry = Bio::EnsEMBL::DBEntry->new( -dbname => $species.'_core_Gene', #-release => $self->db->dnadb->dbc->dbname,
-status => 'KNOWNXREF', #-display_label_linkable => 1,
#-db_display_name => $self->db->dnadb->dbc->dbname,
-db_display_name => 'EnsemblGene', -type => 'MISC', -primary_id => $gene_id, -display_id => $display_name, -info_type => 'MISC', -info_text => 'GENE', -linkage_annotation => 'cisRED search region gene',#omit?
#could have version here if we use the correct dnadb to build the cache
); $dbentry_adaptor->store($dbentry, $search_feature->dbID, 'ExternalFeature', 1);#1 is ignore release flag
} close(SEARCH_REGIONS); $self->log("Stored $cnt cisRED search region ExternalFeatures"); $self->log("Skipped $skipped cisRED search region ExternalFeatures"); $self->log("Skipped an additional $skipped_xref cisRED search region DBEntry imports"); #print "$coords_changed features had their co-ordinates changed as a result of assembly mapping.\n" if ($new_assembly);
return; } 1;
}
General documentation
No general documentation available.