package EnsEMBL::Web::Object::Variation;
use Bio::EnsEMBL::Variation::Utils::Sequence qw(ambiguity_code variation_class);
use Bio::EnsEMBL::Utils::Eprof qw(eprof_start eprof_end eprof_dump);
### Purpose: to store and manipulate ensembl Variation and Variation Feature objects
### it provides a thin wrapper around the ensembl-core-api
=head1 LICENCE
This code is distributed under an Apache style licence:
Please see http://www.ensembl.org/code_licence.html for details
CONTACT Fiona Cunningham - webmaster@sanger.ac.uk
=cut
use strict;
use warnings;
no warnings "uninitialized";
use EnsEMBL::Web::Object;
our @ISA = qw(EnsEMBL::Web::Object);
our $MEMD = new EnsEMBL::Web::Cache;
sub _filename {
my $self = shift;
my $name = sprintf '%s-variation-%d-%s-%s',
$self->species,
$self->species_defs->ENSEMBL_VERSION,
'variation',
$self->name;
$name =~ s/[^-\w\.]/_/g;
return $name;
}
sub availability {
my $self = shift;
my $hash = $self->_availability;
if ($self->Obj->isa('Bio::EnsEMBL::Variation::Variation')){
if ($self->Obj->failed_description) { $hash->{'unmapped'} =1; }
else { $hash->{'variation'} = 1; }
}
return $hash;
}
sub counts {
my $self = shift;
my $obj = $self->Obj;
return {} unless $obj->isa('Bio::EnsEMBL::Variation::Variation');
my $key = '::Counts::Variation::'.
$ENV{ENSEMBL_SPECIES} .'::'.
$self->core_objects->{parameters}{vdb} .'::'.
$self->core_objects->{parameters}{v} .'::';
my $counts;
$counts = $MEMD->get($key) if $MEMD;
unless ($counts) {
$counts = {};
$counts->{'transcripts'} = $self->count_transcripts;
$counts->{'populations'} = $self->count_populations;
$counts->{'individuals'} = $self->count_individuals;
$counts->{'ega'} = $self->count_ega;
$MEMD->set($key, $counts, undef, 'COUNTS') if $MEMD;
}
return $counts;
}
sub count_ega {
my $self = shift;
my @ega_links = @{$self->get_external_data};
my $counts = scalar @ega_links || 0;
return $counts;
}
sub count_transcripts {
my $self = shift;
my %mappings = %{ $self->variation_feature_mapping };
my $counts = 0;
foreach my $varif_id (keys %mappings) {
next unless ($varif_id eq $self->param('vf'));
my @transcript_variation_data = @{ $mappings{$varif_id}{transcript_vari} };
$counts = scalar @transcript_variation_data;
}
return $counts;
}
sub count_populations {
my $self = shift;
my $counts = scalar(keys %{$self->freqs}) || 0;
return $counts;
}
sub count_individuals {
my $self = shift;
my $counts = scalar (keys %{ $self->individual_table }) || 0;
return $counts;
}
sub short_caption {
my $self = shift;
my $label = $self->name;
if( length($label)>30) {
return "Var: $label";
} else {
return "Variation: $label";
}
}
sub caption {
my $self = shift;
my $caption = 'Variation: '.$self->name;
return $caption;
}
# Location ----------------------------------------------------------------------
sub location_string {
### Variation_location
### Example : my $location = $self->location_string;
### Description: Gets chr:start-end for the SNP with 100 bases on either side
### Returns string: chr:start-end
my ($self, $unique) = @_;
my( $sr, $st ) = $self->_seq_region_($unique);
return $sr ? "$sr:@{[$st-100]}-@{[$st+100]}" : undef;
}
sub var_location {
### Variation_location
### Example : my $location = $self->location_string;
### Description: Gets chr:start-end for the SNP
### Returns string: chr:start-end
my ($self, $unique) = @_;
my( $sr, $st ) = $self->_seq_region_($unique);
return $sr ? "$sr:@{[$st]}-@{[$st]}" : undef;
}
sub _seq_region_ {
### Variation_location
### Args : $unique
### if $unique=1 -> returns undef if there are more than one
### variation features returned)
### if $unique is 0 or undef, it returns the data for the first
### mapping postion
### Example : my ($seq_region, $start) = $self->_seq_region_;
### Description: Gets the sequence region, start and coordinate system name
### Returns $seq_region, $start, $seq_type
my $self = shift;
my $unique = shift;
my($seq_region, $start, $seq_type);
if ( my $region = $self->param('c') ) {
($seq_region, $start) = split /:/, $region;
my $slice = $self->database('core')->get_SliceAdaptor->fetch_by_region(undef,$seq_region);
return unless $slice;
$seq_type = $slice->coord_system->name;
}
else {
my @vari_mappings = @{ $self->get_variation_features };
return (undef, undef, undef, "no") unless @vari_mappings;
if ($unique) {
return (undef, undef, undef, "multiple") if $#vari_mappings > 0;
}
$seq_region = $self->region_name($vari_mappings[0]);
$start = $self->start($vari_mappings[0]);
$seq_type = $self->region_type($vari_mappings[0]);
}
return ( $seq_region, $start, $seq_type );
}
sub seq_region_name {
### Variation_location
### a
my( $sr,$st) = $_[0]->_seq_region_; return $sr;
}
sub seq_region_start {
### Variation_location
### a
my( $sr,$st) = $_[0]->_seq_region_; return $st;
}
sub seq_region_end {
### Variation_location
### a
my( $sr,$st) = $_[0]->_seq_region_; return $st;
}
sub seq_region_strand {
### Variation_location
### a
return 1;
}
sub seq_region_type {
### Variation_location
### a
my($sr,$st,$type) = $_[0]->_seq_region_; return $type;
}
sub seq_region_data {
### Variation_location
### Args : none
### Example : my ($seq_region, $start, $type) = $object->seq_region_data;
### Description: Only returns sequence region, start and coordinate system name
### if this Variation Object maps to one Variation Feature obj
### Returns $seq_region, $start, $seq_type, $error(optional) which specifies
### 'no' if no mapping or 'multiple' if has several hits
### If there is an error, the first 3 args returned are undef
my($sr,$st,$type, $error) = $_[0]->_seq_region_(1);
return ($sr, $st, $type, $error);
}
# Variation calls ----------------------------------------------------------------
sub vari {
### Variation_object_calls
### Args: none
### Example : my $ensembl_vari = $object->vari
### Description: Gets the ensembl variation object stored on the variation data object
### Returns Bio::EnsEmbl::Variation
my $self = shift;
return $self->Obj;
}
sub name {
### Variation_object_calls
### a
### Arg (optional): Variation object name (string)
### Example : my $vari_name = $object->vari_name;
### Example : $object->vari_name('12335');
### Returns String for variation name
my $self = shift;
if (@_) {
$self->vari->name(shift);
}
return $self->vari->name;
}
sub source {
### Variation_object_calls
### Args: none
### Example : my $vari_source = $object->source;
### Description: gets the Variation source
### Returns String
$_[0]->vari->source;
}
sub get_genes {
### Variation_object_calls
### a
### Args: none
### Example : my @genes = @ {$obj->get_genes};
### Returns arrayref of Bio::EnsEMBL::Gene objects
$_[0]->vari->get_all_Genes;
}
sub source_version {
### Variation_object_calls
### a
### Example : my $vari_source_version = $object->source
### Description: gets the Variation source version e.g. dbSNP version 119
### Returns String
my $self = shift;
my $source = $self->vari->source;
my $version = $self->vari->adaptor->get_source_version($source);
return $version;
}
sub dblinks {
### Variation_object_calls
### Args: none
### Example : my $dblinks = $object->dblinks;
### Description: gets the SNPs links to external database
### Returns Hashref (external DB => listref of external IDs)
my $self = shift;
my @sources = @{ $self->vari->get_all_synonym_sources };
my %synonyms;
foreach (@sources) {
$synonyms{$_} = $self->vari->get_all_synonyms($_);
}
return \%synonyms;
}
sub consequence_type {
my $self = shift;
my $consequence_type;
my @vari_mappings = @{ $self->get_variation_features };
foreach my $f (@vari_mappings){
return '-' unless $f->variation_name eq $self->name;
$consequence_type = $f->display_consequence;
}
$consequence_type =~s/_/ /g;
return $consequence_type;
}
sub status {
### Variation_object_calls
### a
### Example : my $vari_status = $object->get_all_validation_states;
### Returns List of states
my $self = shift;
return $self->vari->get_all_validation_states;
}
sub flanking_seq {
### Variation_object_calls
### Args: "up" or "down" (string)
### Example : my $down_seq = $object->flanking_seq($down);
### Description: gets the sequence downstream of the SNP
### Returns String
my $self = shift;
my $direction = shift;
my $call = $direction eq 'up' ? "five_prime_flanking_seq" : "three_prime_flanking_seq";
my $sequence;
eval {
$sequence = $self->vari->$call;
};
if ($@) {
warn "*****[ERROR]: No flanking sequence!";
return 'unavailable';
}
return uc($sequence);
}
sub alleles {
### Variation_object_call
### Args: none
### Example : my $alleles = $object->alleles;
### Description: gets the SNP alleles
### Returns Array or string
my $self = shift;
my @vari_mappings = @{ $self->unique_variation_feature };
return $vari_mappings[0]->allele_string if @vari_mappings == 1;
# Several mappings or no mappings
my @allele_obj = @{$self->vari->get_all_Alleles};
my %alleles;
map { $alleles{$_->allele} = 1; } @allele_obj;
my $observed_alleles = "Observed alleles are: ". join ", ", (keys %alleles);
if (@vari_mappings) {
return "$observed_alleles";
} else {
return "This variation has no mapping. $observed_alleles";
}
}
sub vari_class{
### Variation_object_calls
### a
### Example : my $vari_class = $object->vari_class
### Description: returns the variation class (indel, snp, het) for a varation
### Returns String
return $_[0]->vari->var_class;
}
sub moltype {
### Variation_object_calls
### a
### Example : $object->moltype;
### Description: returns the molecular type of the variation
### Returns String
my $self = shift;
return $self->vari->moltype;
}
sub ancestor {
### Variation_object_calls
### a
### Example : $object->ancestral_allele;
### Description: returns the ancestral allele for the variation
### Returns String
my $self = shift;
return $self->vari->ancestral_allele;
}
sub tagged_snp {
### Variation_object_calls
### Args: none
### Example : my $pops = $object->tagged_snp
### Description: The "is_tagged" call returns an array ref of populations
### objects Bio::Ensembl::Variation::Population where this SNP
### is a tag SNP
### Returns hashref of pop_name
my $self = shift;
my @vari_mappings = @{ $self->get_variation_features };
return {} unless @vari_mappings;
my %pops;
foreach my $vf ( @vari_mappings ) {
foreach my $pop_obj ( @{ $vf->is_tagged } ) {
$pops{$self->pop_name($pop_obj)} = "Tag SNP";
}
}
return \%pops or {};
}
sub freqs_hack {
### hacked version of freqs
### Population_allele_genotype_frequencies
### Args : none
### Example : my $data = $object->test_freqs;
### Description: gets allele and genotype frequencies for this Variation
### Returns hash of data,
my $self = shift;
my $allele_list = $self->vari->get_all_Alleles;
my %data;
my %population_row_count;
next unless $self->pop_genotype_obj;
my %populations;
my %populations_alleles;
foreach my $pop_gt_obj ( @{ $self->pop_genotype_obj } ) {
my $pop_id = $pop_gt_obj->population->dbID;
my $allele_string = $pop_id . $pop_gt_obj->allele1 . $pop_gt_obj->allele2;
## Check if we have already seen this allele combination for this population
my $row_number = 1;
if ( $populations_alleles{$allele_string} ) { $row_number = $populations_alleles{$allele_string}; }
my $new_number = $row_number + 1;
$populations_alleles{$allele_string} = $new_number;
my %pop_gt_row;
if ($populations{$pop_id}) { %pop_gt_row = %{ $populations{$pop_id} }; }
my @objects;
if ($pop_gt_row{'row_' .$row_number} ) {
@objects = @{ $pop_gt_row{'row_' .$row_number} };
my $old_dbID = $objects[0]->dbID;
my $current_dbID = $pop_gt_obj->dbID;
if ($old_dbID >= ($current_dbID + 3) || $old_dbID <= ($current_dbID - 3) ){
$row_number = $new_number;
@objects = ();
$new_number++;
$populations_alleles{$allele_string} = $new_number;
}
}
push (@objects, $pop_gt_obj);
$pop_gt_row{'row_' .$row_number} = \@objects;
$populations{$pop_id} = \%pop_gt_row;
}
foreach (keys %populations){
my %rows = %{$populations{$_}};
foreach (values %rows) {
my @pop_gt_objs = @{$_};
my $pop_obj = $pop_gt_objs[0]->population;
my $pop_id = $self->pop_id($pop_obj);
if ($population_row_count{$pop_id}) {
my $count = $population_row_count{$pop_id};
$pop_id .= "_" .$count;
$count++;
$population_row_count{$pop_id} = $count;
}
else { $population_row_count{$pop_id} = 1; }
my (%gt_freqs, %alleles);
foreach my $pop_gt_object (@{$_}) {
my $allele_string = $pop_id .".". $pop_gt_object->allele1 ."|". $pop_gt_object->allele2;
$gt_freqs{$allele_string} = $pop_gt_object->frequency;
$alleles{$pop_gt_object->allele1} = 1;
$alleles{$pop_gt_object->allele2} = 1;
## Add population genotype frequency
push (@{ $data{$pop_id}{GenotypeFrequency} }, $pop_gt_object->frequency);
push (@{ $data{$pop_id}{Genotypes} }, $self->pop_genotypes($pop_gt_object));
next if $data{$pop_id}{pop_info};
$data{$pop_id}{pop_info} = $self->pop_info($pop_obj);
}
## Now work out allele frequencys
foreach my $allele (keys %alleles){
my $allele_total;
foreach my $key ( keys %gt_freqs){
if ($key =~/$allele\|$allele/){
$allele_total = $allele_total + $gt_freqs{$key};
} elsif ($key =~/\w\|$allele/ || $key =~/$allele\|\w/){
my $freq = ($gt_freqs{$key}) * 0.5;
$allele_total = $allele_total + $freq;
} elsif ($key =~/$allele\|/){
$allele_total = $allele_total + $gt_freqs{$key};
}
}
my $allele_freq = $allele_total;
push (@{ $data{$pop_id}{AlleleFrequency} }, $allele_freq);
push (@{ $data{$pop_id}{Alleles} }, $allele);
next if $data{$pop_id}{pop_info};
$data{$pop_id}{pop_info} = $self->pop_info($pop_obj);
}
}
}
return \%data;
}
sub freqs {
### Population_allele_genotype_frequencies
### Args : none
### Example : my $data = $object->freqs;
### Description: gets allele and genotype frequencies for this Variation
### Returns hash of data,
my $self = shift;
my $allele_list = $self->vari->get_all_Alleles;
return {} unless $allele_list;
my %data;
foreach my $allele_obj ( @{ $allele_list } ) {
my $pop_obj = $allele_obj->population;
next unless $pop_obj;
my $pop_id = $self->pop_id($pop_obj);
push (@{ $data{$pop_id}{AlleleFrequency} }, $allele_obj->frequency || "");
push (@{ $data{$pop_id}{Alleles} }, $allele_obj->allele);
next if $data{$pop_id}{pop_info};
$data{$pop_id}{pop_info} = $self->pop_info($pop_obj);
}
# Add genotype data;
return {} unless $self->pop_genotype_obj;
foreach my $pop_gt_obj ( @{ $self->pop_genotype_obj } ) {
my $pop_obj = $pop_gt_obj->population;
my $pop_id = $self->pop_id($pop_obj);
push (@{ $data{$pop_id}{GenotypeFrequency} }, $pop_gt_obj->frequency);
push (@{ $data{$pop_id}{Genotypes} }, $self->pop_genotypes($pop_gt_obj));
next if $data{$pop_id}{pop_info};
$data{$pop_id}{pop_info} = $self->pop_info($pop_obj);
}
return \%data;
}
sub get_external_data {
my $self = shift;
my $v = $self->vari;
my $vdb = $self->DBConnection->get_DBAdaptor('variation');
my $vaa = $vdb->get_VariationAnnotationAdaptor();
my @data = @{$vaa->fetch_all_by_Variation($v)};
return \@data;
}
# Population genotype and allele frequency table calls ----------------
sub pop_genotype_obj {
### frequencies_table
### Example : my $pop_genotype_obj = $object->pop_genotype_obj;
### Description: gets Population genotypes for this Variation
### Returns listref of Bio::EnsEMBL::Variation::PopulationGenotype
my $self = shift;
return $self->vari->get_all_PopulationGenotypes;
}
sub pop_genotypes {
### frequencies_table
### Args : Bio::EnsEMBL::Variation::PopulationGenotype object
### Example : $genotype_freq = $object->pop_genotypes($pop);
### Description: gets the Population genotypes
### Returns String
my ($self, $pop_genotype_obj) = @_;
return join "|", sort($pop_genotype_obj->allele1, $pop_genotype_obj->allele2);
}
sub pop_info {
### frequencies_table
### Args : Bio::EnsEMBL::Variation::Population object
### Example : my $data = $self->pop_info
### Description: returns a hash with data about this population
### Returns hash of data
my $self = shift;
my $pop_obj = shift;
my %data;
$data{Name} = $self->pop_name($pop_obj);
$data{PopLink} = $self->pop_links($pop_obj);
$data{Size} = $self->pop_size($pop_obj);
$data{Description} = $self->pop_description($pop_obj);
$data{"Super-Population"} = $self->extra_pop($pop_obj,"super");
$data{"Sub-Population"} = $self->extra_pop($pop_obj,"sub");
return \%data;
}
sub pop_name {
### frequencies_table
### Args : Bio::EnsEMBL::Variation::Population object
### Example : $object->pop_name($pop);
### Description: gets the Population name
### Returns String
my ($self, $pop_obj) = @_;
return unless $pop_obj;
return $pop_obj->name;
}
sub pop_id {
### frequencies_table
### Args : Bio::EnsEMBL::Variation::Population object
### Example : $object->pop_id($pop);
### Description: gets the Population ID
### Returns String
my ($self, $pop_obj) = @_;
return unless $pop_obj;
return $pop_obj->dbID;
}
sub pop_links {
### frequencies_table
### Args : Bio::EnsEMBL::Variation::Population object
### Example : $genotype_freq = $object->pop_links($pop);
### Description: gets the Population description
### Returns String
my ($self, $pop_obj) = @_;
return $pop_obj->get_all_synonyms("dbSNP");
}
sub pop_size {
### frequencies_table
### Args : Bio::EnsEMBL::Variation::Population object
### Example : $genotype_freq = $object->pop_size($pop);
### Description: gets the Population size
### Returns String
my ($self, $pop_obj) = @_;
return $pop_obj->size;
}
sub pop_description {
### frequencies_table
### Args : Bio::EnsEMBL::Variation::Population object
### Example : $genotype_freq = $object->pop_description($pop);
### Description: gets the Population description
### Returns String
my ($self, $pop_obj) = @_;
return $pop_obj->description;
}
sub extra_pop {
### frequencies_table
### Args1 : Bio::EnsEMBL::Variation::Population object
### Args2 : string "super", "sub"
### Example : $genotype_freq = $object->extra_pop($pop, "super");
### Description: gets any super/sub populations
### Returns String
my ($self, $pop_obj, $type) = @_;
return {} unless $pop_obj;
my $call = "get_all_$type" . "_Populations";
my @populations = @{ $pop_obj->$call};
my %extra_pop;
foreach my $pop ( @populations ) {
my $id = $self->pop_id($pop_obj);
$extra_pop{$id}{Name} = $self->pop_name($pop);
$extra_pop{$id}{Size} = $self->pop_size($pop);
$extra_pop{$id}{PopLink} = $self->pop_links($pop);
$extra_pop{$id}{Description}= $self->pop_description($pop);
}
return \%extra_pop;
}
# Individual table -----------------------------------------------------
sub individual_table {
### individual_table_calls
### Example : my $ind_genotypes = $object->individual_table;
### Description: gets Individual Genotype data for this variation
### Returns hashref with all the data
my $self = shift;
my $individual_genotypes = $self->individual_genotypes_obj;
return {} unless @$individual_genotypes;
my %data;
foreach my $ind_gt_obj ( @$individual_genotypes ) {
my $ind_obj = $ind_gt_obj->individual;
next unless $ind_obj;
my $ind_id = $ind_obj->dbID;
$data{$ind_id}{Name} = $ind_obj->name;
$data{$ind_id}{Genotypes} = $self->individual_genotype($ind_gt_obj);
$data{$ind_id}{Gender} = $ind_obj->gender;
$data{$ind_id}{Description} = $self->individual_description($ind_obj);
$data{$ind_id}{Population} = $self->get_individuals_pops($ind_obj);
$data{$ind_id}{Mother} = $self->parent($ind_obj,"mother");
$data{$ind_id}{Father} = $self->parent($ind_obj,"father");
$data{$ind_id}{Children} = $self->child($ind_obj);
}
return \%data;
}
sub individual_genotypes_obj {
### Individual_genotype_table_calls
### Example : my $ind_genotypes = $object->individual_genotypes;
### Description: gets IndividualGenotypes for this Variation
### Returns listref of IndividualGenotypes
my $self = shift;
my $individuals;
eval {
$individuals = $self->vari->get_all_IndividualGenotypes;
};
if ($@) {
warn "\n\n************ERROR************: Bio::EnsEMBL::Variation::Variation::get_all_IndividualGenotypes fails.";
}
return $individuals;
}
sub individual_genotype {
### Individual_genotype_table_calls
### Args : Bio::EnsEMBL::Variation::IndividualGenotype object
### Example : $genotype_freq = $object->individual_genotypes($individual);
### Description: gets the Individual genotypes
### Returns String
my ($self, $individual) = @_;
return $individual->allele1."|".$individual->allele2;
}
sub individual_description {
### Individual_genotype_table_calls
### Args : Bio::EnsEMBL::Variation::Individual object
### Example : $genotype_freq = $object->individual_description($individual);
### Description: gets the Individual description
### Returns String
my ($self, $individual_obj) = @_;
return $individual_obj->description;
}
sub parent {
### Individual_genotype_table_calls
### Args1 : Bio::EnsEMBL::Variation::Individual object
### Arg2 : string "mother" "father"
### Example : $mother = $object->parent($individual, "mother");
### Description: gets any related individuals
### Returns Bio::EnsEMBL::Variation::Individual
my ($self, $ind_obj, $type) = @_;
my $call = $type. "_Individual";
my $parent = $ind_obj->$call;
return {} unless $parent;
# Gender is obvious, not calling their parents
return { Name => $parent->name,
### Description=> $self->individual_description($ind_obj),
};
}
sub child {
### Individual_genotype_table_calls
### Args : Bio::EnsEMBL::Variation::Individual object
### Example : %children = %{ $object->extra_individual($individual)};
### Description: gets any related individuals
### Returns Bio::EnsEMBL::Variation::Individual
my ($self, $individual_obj) = @_;
my %children;
foreach my $individual ( @{ $individual_obj->get_all_child_Individuals} ) {
my $gender = $individual->gender;
$children{$individual->name} = [$gender,
$self->individual_description($individual)];
}
return \%children;
}
sub get_individuals_pops {
### Individual_genotype_table_calls
### Args : Bio::EnsEMBL::Variation::Individual object
### Example : $pops = $object->get_individuals_pop($individual)};
### Description: gets any individual''s populations
### Returns Bio::EnsEMBL::Variation::Population
my ($self, $individual) = @_;
my @populations = @{$individual->get_all_Populations};
my @pop_string;
foreach (@populations) {
push (@pop_string, {Name => $self->pop_name($_),
Link => $self->pop_links($_)});
}
return \@pop_string;
}
# Variation mapping ###########################################################
sub variation_feature_mapping { ## used for snpview
### Variation_mapping
### Example : my @vari_features = $object->variation_feature_mappin
### Description: gets the Variation features found on a variation object;
### Returns Arrayref of Bio::EnsEMBL::Variation::VariationFeatures
my $self = shift;
my %data;
foreach my $vari_feature_obj (@{ $self->get_variation_features }) {
my $varif_id = $vari_feature_obj->dbID;
$data{$varif_id}{Chr} = $self->region_name($vari_feature_obj);
$data{$varif_id}{start} = $self->start($vari_feature_obj);
$data{$varif_id}{end} = $vari_feature_obj->end;
$data{$varif_id}{strand} = $vari_feature_obj->strand;
$data{$varif_id}{transcript_vari} = $self->transcript_variation($vari_feature_obj);
}
return \%data;
}
# Calls for variation features -----------------------------------------------
sub unique_variation_feature {
### Variation_features
### Description: returns {{Bio::Ensembl::Variation::Feature}} object if
### this {{Bio::Ensembl::Variation}} has a unique mapping
### Returns undef if no mapping
### Returns a arrayref of single Bio::Ensembl::Variation::Feature object if one mapping
### Returns a arrayref of Bio::Ensembl::Variation::Feature object if multiple mapping
my $self = shift;
my @variation_features = @{ $self->get_variation_features || [] };
return [] unless @variation_features;
return \@variation_features unless $#variation_features > 0; # if unique mapping
# Must have multiple mapping
my ($sr, $start, $type) = $self->seq_region_data;
return \@variation_features unless $sr; #$sr undef if no unique mapping
my @return;
foreach (@variation_features) { # try to find vf which matches unique mapping
next unless $self->start($_) eq $start;
next unless $self->region_name($_) eq $sr;
next unless $self->region_type($_) eq $type;
push @return, $_;
}
return \@return;
}
sub get_variation_features {
### Variation_features
### Example : my @vari_features = $object->get_variation_features;
### Description: gets the Variation features found on a variation object;
### Returns Arrayref of Bio::EnsEMBL::Variation::VariationFeatures
my $self = shift;
return [] unless $self->vari;
# return VariationFeatures that were added by add_variation_feature if
# present
return $self->{'_variation_features'} if ($self->{'_variation_features'});
my $dbs = $self->DBConnection->get_DBAdaptor('variation');
my $vari_f_adaptor = $dbs->get_VariationFeatureAdaptor;
my $vari_features = $vari_f_adaptor->fetch_all_by_Variation($self->vari);
# warn Data::Dumper::Dumper($vari_features);
return $vari_features || [];
}
sub add_variation_feature {
### Variation_features
### Args : a Bio::EnsEBML::Variation::VariationFeature object
### Example : $object->add_variation_feature($varfeat);
### Description: adds a VariationFeature to the Variation
### Returns none
### Exceptions : thrown if wrong object supplied
my ($self, $vari_feature) = @_;
unless ($vari_feature->isa('Bio::EnsEMBL::Variation::VariationFeature')) {
# throw
$self->problem('fatal', 'EnsEMBL::Web::Data::SNP->add_variation_feature expects a Bio::EnsEMBL::Variation::VariationFeature as argument');
}
push @{ $self->{'_variation_features'} }, $vari_feature;
}
sub region_type {
### Variation_features
### Args : Bio::EnsEMBL::Variation::Variation::Feature
### Example : my $chr = $data->region_name($vari)
### Description: gets the VariationFeature slice seq region name
### Returns String
my ($self, $vari_feature) = @_;
my $slice = $vari_feature->slice;
return $slice->coord_system->name if $slice;
}
sub region_name {
my ($self, $vari_feature) = @_;
my $slice = $vari_feature->slice;
return $slice->seq_region_name() if $slice;
}
sub start {
### Variation_features
### Args : Bio::EnsEMBL::Variation::Variation::Feature
### Example : my $vari_start = $object->start($vari);
### Description: gets the Variation start coordinates
### Returns String
my ($self, $vari_feature) = @_;
return $vari_feature->start;
}
sub transcript_variation {
### Variation_features
### Args : Bio::EnsEMBL::Variation::Variation::Feature
### Example : my $consequence = $object->consequence($vari);
### Description: returns SNP consequence (synonymous, stop gained, ...)
### Returns arrayref of transcript variation objs
my ($self, $vari_feature) = @_;
my $dbs = $self->DBConnection->get_DBAdaptor('variation');
$dbs->dnadb($self->database('core'));
my $transcript_variation_obj = $vari_feature->get_all_TranscriptVariations;
return [] unless $transcript_variation_obj;
my @data;
foreach my $tvari_obj ( @{ $transcript_variation_obj } ) {
next unless $tvari_obj->transcript;
my $type = join ", " , @{ $tvari_obj->consequence_type || [] };
push (@data, {
conseq => $type,
transcriptname => $tvari_obj->transcript->stable_id,
proteinname => $tvari_obj->transcript->translation ? $tvari_obj->transcript->translation->stable_id : '-',
cdna_start => $tvari_obj->cdna_start,
cdna_end => $tvari_obj->cdna_end,
translation_start =>$tvari_obj->translation_start,
translation_end => $tvari_obj->translation_end,
pepallele => $tvari_obj->pep_allele_string,
});
}
return \@data;
}
# LD stuff ###################################################################
sub ld_pops_for_snp {
### LD
### Description: gets an LDfeature container for this SNP and calls all the populations on this
### Returns array ref of population IDs
my $self = shift;
my @vari_mappings = @{ $self->unique_variation_feature };
return [] unless @vari_mappings;
my @pops;
foreach ( @vari_mappings ) {
my $ldcontainer = $_->get_all_LD_values; #warn scalar @{$ldcontainer->get_all_populations};
push @pops, @{$ldcontainer->get_all_populations};
}
return \@pops;
}
sub find_location {
### LD
### Example : my $data = $object->find_location
### Description: returns the genomic location for the current slice
### Returns hash of data
my $self = shift;
my $width = shift || $self->param('w') || 50000;
unless ( $self->{'_slice'} ) {
$self->_get_slice($width);
}
my $slice = $self->{'_slice'};
return {} unless $slice;
return $slice->name;
}
sub pop_obj_from_id {
### LD
### Args : Population ID
### Example : my $pop_name = $object->pop_obj_from_id($pop_id);
### Description: returns population name for the given population dbID
### Returns population object
my $self = shift;
my $pop_id = shift;
my $variation_db = $self->database('variation')->get_db_adaptor('variation');
my $pa = $variation_db->get_PopulationAdaptor;
my $pop_obj = $pa->fetch_by_dbID($pop_id);
return {} unless $pop_obj;
my %data;
$data{$pop_id}{Name} = $self->pop_name($pop_obj);
$data{$pop_id}{Size} = $self->pop_size($pop_obj);
$data{$pop_id}{PopLink} = $self->pop_links($pop_obj);
$data{$pop_id}{Description}= $self->pop_description($pop_obj);
$data{$pop_id}{PopObject}= $pop_obj; ## ok maybe this is cheating..
return \%data;
}
sub get_default_pop_name {
### LD
### Example: my $pop_id = $object->get_default_pop_name
### Description: returns population id for default population for this species
### Returns population dbID
my $self = shift;
my $variation_db = $self->database('variation')->get_db_adaptor('variation');
my $pop_adaptor = $variation_db->get_PopulationAdaptor;
return unless $pop_adaptor;
my $pop = $pop_adaptor->fetch_default_LDPopulation();
return unless $pop;
return [ $self->pop_name($pop) ];
}
sub location { return $_[0]; }
sub generate_query_hash {
my $self = shift;
return {
'h' => $self->highlights_string,
'source' => $self->source || "dbSNP",
'snp' => $self->name,
'c' => $self->param('c'),
'pop' => $self->get_default_pop_name,
};
}
sub get_source {
my $self = shift;
my $default = shift;
my $vari_adaptor = $self->Obj->adaptor->db->get_db_adaptor('variation');
unless ($vari_adaptor) {
warn "ERROR: Can't get variation adaptor";
return ();
}
if ($default) {
return $vari_adaptor->get_VariationAdaptor->get_default_source();
}
else {
return $vari_adaptor->get_VariationAdaptor->get_all_sources();
}
}
1;