Raw content of Bio::Align::Utilities
<![CDATA[# $Id: Utilities.pm,v 1.8 2002/11/11 18:39:19 jason Exp $
#
# BioPerl module for Bio::Align::Utilities
#
# Cared for by Jason Stajich <jason@bioperl.org>
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Align::Utilities - A collection of utilities regarding converting and manipulating alignment objects
=head1 SYNOPSIS
use Bio::Align::Utilities qw(aa_to_dna_aln);
my $dna_aln = aa_to_dna_aln($aaaln,\%dnaseqs);
=head1 DESCRIPTION
This module contains utility methods for manipulating sequence
alignments ( L<Bio::Align::AlignI>) objects.
The B<aa_to_dna_aln> utility is essentially the same as the B<mrtrans>
program by Bill Pearson available at
ftp://ftp.virginia.edu/pub/fasta/other/mrtrans.shar. Of course this
is a pure-perl implementation, but just to mention that if anything
seems odd you can check the alignments generated against Bill's
program.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/MailList.shtml - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
email or the web:
bioperl-bugs@bioperl.org
http://bugzilla.bioperl.org/
=head1 AUTHOR - Jason Stajich
Email jason@bioperl.org
=head1 CONTRIBUTORS
Additional contributors names and emails here
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
#' keep my emacs happy
# Let the code begin...
package Bio::Align::Utilities;
use vars qw(@ISA @EXPORT @EXPORT_OK);
use strict;
use Carp;
require Exporter;
@ISA = qw(Exporter);
@EXPORT = qw();
@EXPORT_OK = qw(aa_to_dna_aln);
use constant CODONSIZE => 3;
=head2 aa_to_dna_aln
Title : aa_to_dna_aln
Usage : my $dnaaln = aa_to_dna_aln($aa_aln, \%seqs);
Function: Will convert an AA alignment to DNA space given the
corresponding DNA sequences. Note that this method expects
the DNA sequences to be in frame +1 (GFF frame 0) as it will
start to project into coordinates starting at the first base of
the DNA sequence, if this alignment represents a different
frame for the cDNA you will need to edit the DNA sequences
to remove the 1st or 2nd bases (and revcom if things should be).
Returns : Bio::Align::AlignI object
Args : 2 arguments, the alignment and a hashref.
Alignment is a Bio::Align::AlignI of amino acid sequences.
The hash reference should have keys which are
the display_ids for the aa
sequences in the alignment and the values are a
Bio::PrimarySeqI object for the corresponding
spliced cDNA sequence.
See also: L<Bio::Align::AlignI>, L<Bio::SimpleAlign>, L<Bio::PrimarySeq>
=cut
sub aa_to_dna_aln {
my ($aln,$dnaseqs) = @_;
unless( defined $aln &&
ref($aln) &&
$aln->isa('Bio::Align::AlignI') ) {
croak('Must provide a valid Bio::Align::AlignI object as the first argument to aa_to_dna_aln, see the documentation for proper usage and the method signature');
}
my $alnlen = $aln->length;
#print "HSP length is $alnlen\n";
my $dnaalign = new Bio::SimpleAlign;
foreach my $seq ( $aln->each_seq ) {
my $newseq;
my $dnaseq = $dnaseqs->{$seq->display_id} || croak("cannot find ".
$seq->display_id);
foreach my $pos ( 1..$alnlen ) {
my $loc = $seq->location_from_column($pos);
my $dna = '';
if( !defined $loc || $loc->location_type ne 'EXACT' ) {
$dna = '---';
} else {
# To readjust to codon boundaries
# end needs to be +1 so we can just multiply by CODONSIZE
# to get this
my ($start,$end) = ((($loc->start - 1)* CODONSIZE) +1,
($loc->end)* CODONSIZE);
if( $start <=0 || $end > $dnaseq->length() ) {
print STDERR "start is ", $loc->start, " end is ", $loc->end, " while dnaseq length is ", $dnaseq->length(), " and start/end projected are $start,$end \n";
warn("codons don't seem to be matching up for $start,$end");
$dna = '---';
} else {
$dna = $dnaseq->subseq($start,$end);
}
}
$newseq .= $dna;
}
# funky looking math is to readjust to codon boundaries and deal
# with fact that sequence start with 1
my $newdna = new Bio::LocatableSeq(-display_id => $seq->id(),
-start => (($seq->start - 1) *
CODONSIZE) + 1,
-end => ($seq->end * CODONSIZE),
-strand => $seq->strand,
-seq => $newseq);
$dnaalign->add_seq($newdna);
}
return $dnaalign;
}
1;
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