Bio::AlignIO
meme
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Summary
Bio::AlignIO::meme - meme sequence input/output stream
Package variables
Privates (from "my" definitions)
$MEME_VERS_ERR = "MEME output file must be generated by version 3.0 or higher"
$MEME_NO_HEADER_ERR = "MEME output file contains no header line (ex: MEME version 3.0)"
$HTML_VERS_ERR = "MEME output file must be generated with the -text option"
Included modules
Inherit
Synopsis
Do not use this module directly. Use it via the Bio::AlignIO class.
Description
This object transforms the "sites sorted by p-value" sections of a meme
(text) output file into a series of Bio::SimpleAlign objects. Each
SimpleAlign object contains Bio::LocatableSeq objects which represent the
individual aligned sites as defined by the central portion of the "site"
field in the meme file. The start and end coordinates are derived from
the "Start" field. See
Bio::SimpleAlign and
Bio::LocatableSeq for
more information.
This module can only parse MEME version 3.0 and greater. Previous versions
have output formats that are more difficult to parse correctly. If the meme
output file is not version 3.0 or greater, we signal an error.
Methods
Methods description
Title : next_aln Usage : $aln = $stream->next_aln() Function: returns the next alignment in the stream Returns : Bio::SimpleAlign object Args : NONE |
Title : write_aln Usage : $stream->write_aln(@aln) Function: Not implemented Returns : 1 for success and 0 for error Args : Bio::SimpleAlign object |
Methods code
sub _initialize
{ my($self,@args) = @_;
$self->SUPER::_initialize(@args);
$self->{'seen_header'} = 0;
}
1; } |
sub next_aln
{ my ($self) = @_;
my $aln = Bio::SimpleAlign->new(-source => 'meme');
my $line;
my $good_align_sec = 0;
my $in_align_sec = 0;
while (!$good_align_sec && defined($line = $self->_readline()))
{
if (!$in_align_sec)
{
if ($line =~ /^\s*[Mm][Ee][Mm][Ee]\s+version\s+((?:\d+)?\.\d+)/)
{
$self->{'meme_vers'} = $1;
$self->throw($MEME_VERS_ERR) unless ($self->{'meme_vers'} >= 3.0);
$self->{'seen_header'} = 1;
}
if ($line =~ /\<[Tt][Ii][Tt][Ll][Ee]\>/)
{
$self->throw($HTML_VERS_ERR);
}
if ($line =~ /sites sorted by position/) {
$self->throw($MEME_NO_HEADER_ERR) unless ($self->{'seen_header'});
$in_align_sec = 1;
}
}
elsif ($line =~ /^\s*(\S+)\s+([+-])\s+(\d+)\s+(\S+)\s+([\.ACTGactg]*) ([ACTGactg]+) ([\.ACTGactg]*)/)
{
my $seq_name = $1;
my $strand = ($2 eq '+') ? 1 : -1;
my $start_pos = $3;
my $central = uc($6);
my $seq_res = $central;
my $seq_len = length($seq_res);
my $start_coord = $start_pos;
my $end_coord = $start_coord + $seq_len - 1;
my $seq = new Bio::LocatableSeq('-seq'=>$seq_res,
'-id'=>$seq_name,
'-start'=>$start_coord,
'-end'=>$end_coord,
'-strand'=>$strand);
$aln->add_seq($seq);
}
elsif (($line =~ /^\-/) || ($line =~ /Sequence name/))
{
}
elsif ($line =~ /^\s*$/)
{
$in_align_sec = 0;
$good_align_sec = 1;
}
else
{
$self->warn("Unrecognized format:\n$line");
return 0;
}
}
$self->throw($MEME_NO_HEADER_ERR) unless ($self->{'seen_header'});
return (($good_align_sec) ? $aln : 0); } |
sub write_aln
{ my ($self,@aln) = @_;
$self->throw("AlignIO::meme::write_aln not implemented");
return 0;
}
} |
General documentation
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution.
Bug reports can be submitted via email or the web:
bioperl-bugs@bio.perl.org
http://bugzilla.bioperl.org/
AUTHORS - Benjamin Berman | Top |
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with an
underscore.