Bio::AlignIO
psi
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Summary
Bio::AlignIO::psi - Read/Write PSI-BLAST profile alignment files
Package variables
No package variables defined.
Included modules
Inherit
Synopsis
This module will parse PSI-BLAST output of the format seqid XXXX
Description
Describe the object here
Methods
Methods description
Title : next_aln Usage : $aln = $stream->next_aln() Function: returns the next alignment in the stream Returns : Bio::Align::AlignI object Args : NONE |
Title : write_aln Usage : $stream->write_aln(@aln) Function: writes the NCBI psi-format object (.aln) into the stream Returns : 1 for success and 0 for error Args : Bio::Align::AlignI object |
Methods code
sub next_aln
{ my ($self) = @_;
my $aln;
my %seqs;
my @order;
while( defined ($_ = $self->_readline ) ) {
next if( /^\s+$/);
if( !defined $aln ) {
$aln = new Bio::SimpleAlign;
}
my ($id,$s) = split;
push @order, $id if( ! defined $seqs{$id});
$seqs{$id} .= $s;
}
foreach my $id ( @order) {
my $seq = new Bio::LocatableSeq(-seq => $seqs{$id},
-id => $id,
-start => 1,
-end => length($seqs{$id}));
$aln->add_seq($seq);
}
return $aln; } |
sub write_aln
{ my ($self,$aln) = @_;
unless( defined $aln && ref($aln) &&
$aln->isa('Bio::Align::AlignI') ) {
$self->warn("Must provide a valid Bio::Align::AlignI to write_aln");
return 0;
}
my $ct = 0;
my @seqs = $aln->each_seq;
my $len = 1;
my $alnlen = $aln->length;
my $idlen = $IdLength;
my @ids = map { substr($_->display_id,0,$idlen) } @seqs;
while( $len < $alnlen ) {
my $start = $len;
my $end = $len + $BlockLen;
if( $end > $alnlen ) { $end = $alnlen; }
my $c = 0;
foreach my $seq ( @seqs ) {
$self->_print(sprintf("%-".$idlen."s %s\n",
$ids[$c++],
$seq->subseq($start,$end)));
}
$self->_print("\n");
$len += $BlockLen+1;
}
$self->flush if $self->_flush_on_write && defined $self->_fh;
return 1;
}
1; } |
General documentation
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AUTHOR - Jason Stajich | Top |
Additional contributors names and emails here
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
Title : new
Usage : my $obj = new Bio::AlignIO::psi();
Function: Builds a new Bio::AlignIO::psi object
Returns : Bio::AlignIO::psi
Args :