Bio::Location Split
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Summary
Bio::Location::Split - Implementation of a Location on a Sequence
which has multiple locations (start/end points)
Package variables
No package variables defined.
Included modules
Bio::Location::Atomic
Bio::Location::SplitLocationI
Bio::Root::Root
Inherit
Bio::Location::Atomic Bio::Location::SplitLocationI
Synopsis
    use Bio::Location::Split;

    my $splitlocation = new Bio::Location::Split();
    $splitlocation->add_sub_Location(new Bio::Location::Simple(-start=>1,
							       -end=>30,
							       -strand=>1));
    $splitlocation->add_sub_Location(new Bio::Location::Simple(-start=>50,
							       -end=>61,
							       -strand=>1));   
    my @sublocs = $splitlocation->sub_Location();

    my $count = 1;
    # print the start/end points of the sub locations
    foreach my $location ( sort { $a->start <=> $b->start } 
			   @sublocs ) {
	printf "sub feature %d [%d..%d]\n", 
	       $count, $location->start,$location->end, "\n";
        $count++;
    }
Description
This implementation handles locations which span more than one
start/end location, or and/or lie on different sequences.
Methods
BEGIN Code
add_sub_LocationDescriptionCode
each_LocationDescriptionCode
endDescriptionCode
end_pos_typeDescriptionCode
is_single_sequenceDescriptionCode
max_endDescriptionCode
max_startDescriptionCode
min_endDescriptionCode
min_startDescriptionCode
new
No description
Code
seq_idDescriptionCode
splittypeDescriptionCode
startDescriptionCode
start_pos_typeDescriptionCode
strandDescriptionCode
sub_LocationDescriptionCode
to_FTstringDescriptionCode
Methods description
add_sub_Locationcode    nextTop
 Title   : add_sub_Location
 Usage   : $splitloc->add_sub_Location(@locationIobjs);
 Function: add an additional sublocation
 Returns : number of current sub locations
 Args    : list of Bio::LocationI implementing object(s) to add
each_LocationcodeprevnextTop
 Title   : each_Location
 Usage   : @locations = $locObject->each_Location($order);
 Function: Conserved function call across Location:: modules - will
           return an array containing the component Location(s) in
           that object, regardless if the calling object is itself a
           single location or one containing sublocations.
 Returns : an array of Bio::LocationI implementing objects
 Args    : Optional sort order to be passed to sub_Location()
endcodeprevnextTop
  Title   : end
  Usage   : $end = $location->end();
  Function: get the ending point of the last (sorted) sublocation
  Returns : integer
  Args    : none
end_pos_typecodeprevnextTop
  Title   : end_pos_type
  Usage   : my $end_pos_type = $location->end_pos_type();
  Function: Get end position type (ie <,>, ^) 
  Returns : type of position coded as text 
            ('BEFORE', 'AFTER', 'EXACT','WITHIN', 'BETWEEN')
  Args    : none
is_single_sequencecodeprevnextTop
  Title   : is_single_sequence
  Usage   : if($splitloc->is_single_sequence()) {
                print "Location object $splitloc is split ".
                      "but only across a single sequence\n";
	    }
  Function: Determine whether this location is split across a single or
            multiple sequences.

            This implementation ignores (sub-)locations that do not define
            seq_id(). The same holds true for the root location.

  Returns : TRUE if all sublocations lie on the same sequence as the root
            location (feature), and FALSE otherwise.
  Args    : none
max_endcodeprevnextTop
  Title   : max_end
  Usage   : my $maxend = $location->max_end();
  Function: Get maximum ending location of feature endpoint 
  Returns : integer or undef if no maximum ending point.
  Args    : none
max_startcodeprevnextTop
  Title   : max_start
  Usage   : my $maxstart = $location->max_start();
  Function: Get maximum starting location of feature startpoint  
  Returns : integer or undef if no maximum starting point.
  Args    : none
min_endcodeprevnextTop
  Title   : min_end
  Usage   : my $minend = $location->min_end();
  Function: Get minimum ending location of feature endpoint 
  Returns : integer or undef if no minimum ending point.
  Args    : none
min_startcodeprevnextTop
  Title   : min_start
  Usage   : $min_start = $location->min_start();
  Function: get the minimum starting point
  Returns : the minimum starting point from the contained sublocations
  Args    : none
seq_idcodeprevnextTop
  Title   : seq_id
  Usage   : my $seqid = $location->seq_id();
  Function: Get/Set seq_id that location refers to

            We override this here in order to propagate to all sublocations
            which are not remote (provided this root is not remote either)
  Returns : seq_id
  Args    : [optional] seq_id value to set
splittypecodeprevnextTop
  Title   : splittype
  Usage   : $splittype = $fuzzy->splittype();
  Function: get/set the split splittype
  Returns : the splittype of split feature (join, order)
  Args    : splittype to set
startcodeprevnextTop
  Title   : start
  Usage   : $start = $location->start();
  Function: get the starting point of the first (sorted) sublocation
  Returns : integer
  Args    : none
start_pos_typecodeprevnextTop
  Title   : start_pos_type
  Usage   : my $start_pos_type = $location->start_pos_type();
  Function: Get start position type (ie <,>, ^) 
  Returns : type of position coded as text 
            ('BEFORE', 'AFTER', 'EXACT','WITHIN', 'BETWEEN')
  Args    : none
strandcodeprevnextTop
 Title   : strand
 Usage   : $obj->strand($newval)
 Function: For SplitLocations, setting the strand of the container
           (this object) is a short-cut for setting the strand of all
           sublocations.

           In get-mode, checks if no sub-location is remote, and if
           all have the same strand. If so, it returns that shared
           strand value. Otherwise it returns undef.

 Example : 
 Returns : on get, value of strand if identical between sublocations 
           (-1, 1, or undef)
 Args    : new value (-1 or 1, optional)
sub_LocationcodeprevnextTop
 Title   : sub_Location
 Usage   : @sublocs = $splitloc->sub_Location();
 Function: Returns the array of sublocations making up this compound (split)
           location. Those sublocations referring to the same sequence as
           the root split location will be sorted by start position (forward
           sort) or end position (reverse sort) and come first (before
           those on other sequences).

           The sort order can be optionally specified or suppressed by the
           value of the first argument. The default is no sort.

 Returns : an array of Bio::LocationI implementing objects
 Args    : Optionally 1, 0, or -1 for specifying a forward, no, or reverse
           sort order
to_FTstringcodeprevnextTop
  Title   : to_FTstring
  Usage   : my $locstr = $location->to_FTstring()
  Function: returns the FeatureTable string of this location
  Returns : string
  Args    : none
Methods code
BEGINTop
BEGIN {
     # as defined by BSANE 0.03
    @CORBALOCATIONOPERATOR= ('NONE','JOIN', undef, 'ORDER');
}
add_sub_LocationdescriptionprevnextTop
sub add_sub_Location {
    my ($self,@args) = @_;
    my @locs;    
    foreach my $loc ( @args ) {
	if( !ref($loc) || ! $loc->isa('Bio::LocationI') ) {
	    $self->throw("Trying to add $loc as a sub Location but it doesn't implement Bio::LocationI!");
	    next;
	}	
	push @{$self->{'_sublocations'}}, $loc;
    }

    return scalar @{$self->{'_sublocations'}};
}
each_LocationdescriptionprevnextTop
sub each_Location {
    my ($self, $order) = @_;
    my @locs = ();
    foreach my $subloc ($self->sub_Location($order)) {
	# Recursively check to get hierarchical split locations:
	push @locs, $subloc->each_Location($order);
    }
    return @locs;
}
enddescriptionprevnextTop
sub end {
    my ($self,$value) = @_;    
    if( defined $value ) {
	$self->throw("Trying to set the ending point of a split location, that is not possible, try manipulating the sub Locations");
    }
    return $self->SUPER::end();
}
end_pos_typedescriptionprevnextTop
sub end_pos_type {
    my ($self,$value) = @_;

    if( defined $value ) {
	$self->throw("Trying to set end_pos_type of a split location, that is not possible, try manipulating the sub Locations");
    }
    my @locs = $self->sub_Location();
    return ( @locs ) ? $locs[0]->end_pos_type() : undef;
}
is_single_sequencedescriptionprevnextTop
sub is_single_sequence {
    my ($self) = @_;

    my $seqid = $self->seq_id();
    foreach my $loc ($self->sub_Location(0)) {
	$seqid = $loc->seq_id() if(! $seqid);
	if(defined($loc->seq_id()) && ($loc->seq_id() ne $seqid)) {
	    return 0;
	}
    }
    return 1;
}
max_enddescriptionprevnextTop
sub max_end {
    my ($self,$value) = @_;

    if( defined $value ) {
	$self->throw("Trying to set the maximum end point of a split location, that is not possible, try manipulating the sub Locations");
    }
    # reverse sort locations by largest ending to smallest ending
    my @locs = $self->sub_Location(-1);
    return $locs[0]->max_end() if @locs; 
    return undef;
}
max_startdescriptionprevnextTop
sub max_start {
    my ($self,$value) = @_;

    if( defined $value ) {
	$self->throw("Trying to set the maximum starting point of a split location, that is not possible, try manipulating the sub Locations");
    }
    my @locs = $self->sub_Location(1);
    return $locs[0]->max_start() if @locs; 
    return undef;
}
min_enddescriptionprevnextTop
sub min_end {
    my ($self,$value) = @_;

    if( defined $value ) {
	$self->throw("Trying to set the minimum end point of a split location, that is not possible, try manipulating the sub Locations");
    }
    # reverse sort locations by largest ending to smallest ending
    my @locs = $self->sub_Location(-1);
    return $locs[0]->min_end() if @locs; 
    return undef;
}
min_startdescriptionprevnextTop
sub min_start {
    my ($self, $value) = @_;    

    if( defined $value ) {
	$self->throw("Trying to set the minimum starting point of a split location, that is not possible, try manipulating the sub Locations");
    }
    my @locs = $self->sub_Location(1);
    return $locs[0]->min_start() if @locs; 
    return undef;
}
newdescriptionprevnextTop
sub new {
    my ($class, @args) = @_;
    my $self = $class->SUPER::new(@args);
    # initialize
    $self->{'_sublocations'} = [];
    my ( $type, $seqid, $locations ) = 
	$self->_rearrange([qw(SPLITTYPE
                              SEQ_ID
			      LOCATIONS
                              )], @args);
    if( defined $locations && ref($locations) =~ /array/i ) {
	$self->add_sub_Location(@$locations);
    }
    $seqid  && $self->seq_id($seqid);
    $type = lc ($type);    
    $self->splittype($type || 'JOIN');
    return $self;
}
seq_iddescriptionprevnextTop
sub seq_id {
    my ($self, $seqid) = @_;

    if(! $self->is_remote()) {
	foreach my $subloc ($self->sub_Location(0)) {
	    $subloc->seq_id($seqid) if ! $subloc->is_remote();
	}
    }
    return $self->SUPER::seq_id($seqid);
}
splittypedescriptionprevnextTop
sub splittype {
    my ($self, $value) = @_;
    if( defined $value || ! defined $self->{'_splittype'} ) {
	$value = 'JOIN' unless( defined $value );
	$self->{'_splittype'} = uc ($value);
    }
    return $self->{'_splittype'};
}
startdescriptionprevnextTop
sub start {
    my ($self,$value) = @_;    
    if( defined $value ) {
	$self->throw("Trying to set the starting point of a split location, that is not possible, try manipulating the sub Locations");
    }
    return $self->SUPER::start();
}
start_pos_typedescriptionprevnextTop
sub start_pos_type {
    my ($self,$value) = @_;

    if( defined $value ) {
	$self->throw("Trying to set the start_pos_type of a split location, that is not possible, try manipulating the sub Locations");
    }
    my @locs = $self->sub_Location();
    return ( @locs ) ? $locs[0]->start_pos_type() : undef;
}
stranddescriptionprevnextTop
sub strand {
    my ($self,$value) = @_;
    if( defined $value) {
	$self->{'strand'} = $value;
	# propagate to all sublocs
	foreach my $loc ($self->sub_Location(0)) {
	    $loc->strand($value) if ! $loc->is_remote();
	}
    } else {
	my ($strand, $lstrand);
	foreach my $loc ($self->sub_Location(0)) {
	    # we give up upon any location that's remote or doesn't have
	    # the strand specified, or has a differing one set than 
	    # previously seen.
	    # calling strand() is potentially expensive if the subloc is also
	    # a split location, so we cache it
	    $lstrand = $loc->strand();
	    if((! $lstrand) ||
	       ($strand && ($strand != $lstrand)) ||
	       $loc->is_remote()) {
		$strand = undef;
		last;
	    } elsif(! $strand) {
		$strand = $lstrand;
	    }
	}
	return $strand;
    }
}
sub_LocationdescriptionprevnextTop
sub sub_Location {
    my ($self, $order) = @_;
    $order = 0 unless defined $order;
    if( defined($order) && ($order !~ /^-?\d+$/) ) {
	$self->throw("value $order passed in to sub_Location is $order, an invalid value");
    } 
    $order = 1 if($order > 1);
    $order = -1 if($order < -1);

    my @sublocs = defined $self->{'_sublocations'} ? @{$self->{'_sublocations'}} : ();

    # return the array if no ordering requested
    return @sublocs if( ($order == 0) || (! @sublocs) );
    
    # sort those locations that are on the same sequence as the top (`master')
    # if the top seq is undefined, we take the first defined in a sublocation
    my $seqid = $self->seq_id();
    my $i = 0;
    while((! defined($seqid)) && ($i <= $#sublocs)) {
	$seqid = $sublocs[$i++]->seq_id();
    }
    if((! $self->seq_id()) && $seqid) {
	$self->warn("sorted sublocation array requested but ".
		    "root location doesn't define seq_id ".
		    "(at least one sublocation does!)");
    }
    my @locs = ($seqid ?
		grep { $_->seq_id() eq $seqid; } @sublocs :
		@sublocs);
    if(@locs) {
      if($order == 1) {
	  # Schwartzian transforms for performance boost	  
	  @locs = map { $_->[0] }
	  sort { (defined $a && defined $b) ? 
		     $a->[1] <=> $b->[1] : $a ? -1 : 1 }
	  map { [$_, $_->start] } @locs;

      } else { # $order == -1
	@locs = map {$_->[0]}
	        sort { 
		    (defined $a && defined $b) ? 
			$b->[1] <=> $a->[1] : $a ? -1 : 1 }
 	        map { [$_, $_->end] } @locs;
      }
    }
    # push the rest unsorted
    if($seqid) {
	push(@locs, grep { $_->seq_id() ne $seqid; } @sublocs);
    }
    # done!
    return @locs;
}
to_FTstringdescriptionprevnextTop
sub to_FTstring {
    my ($self) = @_;
    my @strs;
    foreach my $loc ( $self->sub_Location() ) {	
	my $str = $loc->to_FTstring();
	# we only append the remote seq_id if it hasn't been done already
	# by the sub-location (which it should if it knows it's remote)
	# (and of course only if it's necessary)
	if( (! $loc->is_remote) &&
	    defined($self->seq_id) && defined($loc->seq_id) &&
	    ($loc->seq_id ne $self->seq_id) ) {
	    $str = sprintf("%s:%s", $loc->seq_id, $str);
	} 
	push @strs, $str;
    }    

    my $str = sprintf("%s(%s)",lc $self->splittype, join(",", @strs));
    return $str;
}

# we'll probably need to override the RangeI methods since our locations will
# not be contiguous.

1;
}
General documentation
FEEDBACKTop
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
  bioperl-l@bioperl.org             - General discussion
  http://bio.perl.org/MailList.html - About the mailing lists
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via email
or the web:
  bioperl-bugs@bio.perl.org
  http://bugzilla.bioperl.org/
AUTHOR - Jason StajichTop
Email jason@bioperl.org
APPENDIXTop
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
LocationI methodsTop
coordinate_policyTop
  Title   : coordinate_policy
  Usage   : $policy = $location->coordinate_policy();
            $location->coordinate_policy($mypolicy); # set may not be possible
  Function: Get the coordinate computing policy employed by this object.

            See Bio::Location::CoordinatePolicyI for documentation about
            the policy object and its use.

            The interface *does not* require implementing classes to accept
            setting of a different policy. The implementation provided here
            does, however, allow to do so.

            Implementors of this interface are expected to initialize every
            new instance with a CoordinatePolicyI object. The implementation
            provided here will return a default policy object if none has
            been set yet. To change this default policy object call this
            method as a class method with an appropriate argument. Note that
            in this case only subsequently created Location objects will be
            affected.

  Returns : A Bio::Location::CoordinatePolicyI implementing object.
  Args    : On set, a Bio::Location::CoordinatePolicyI implementing object.