Bio::SearchIO::Writer HSPTableWriter
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Summary
Bio::SearchIO::Writer::HSPTableWriter - Tab-delimited data for Bio::Search::HSP::HSPI objects
Package variables
Privates (from "my" definitions)
%column_map = ( 'query_name' => ['1', 'result', 'query_name', 's', 'QUERY' ], 'query_length' => ['2', 'result', 'query_length', 'd', 'LEN_Q'], 'hit_name' => ['3', 'hit', 'name', 's', 'HIT'], 'hit_length' => ['4', 'hit', 'hit_length', 'd', 'LEN_H'], 'round' => ['5', 'hit', 'iteration', 'd', 'ROUND', 'hit'], 'rank' => ['6', 'hsp', 'rank', 'd', 'RANK'], 'expect' => ['7', 'hsp', 'expect', '.1e', 'EXPCT'], 'score' => ['8', 'hsp', 'score', 'd', 'SCORE'], 'bits' => ['9', 'hsp', 'bits', 'd', 'BITS'], 'frac_identical_query' => ['10', 'hsp', 'frac_identical/query', '.2f', 'FR_IDQ'], 'frac_identical_hit' => ['11', 'hsp', 'frac_identical/hit', '.2f', 'FR_IDH'], 'frac_conserved_query' => ['12', 'hsp', 'frac_conserved/query', '.2f', 'FR_CNQ'], 'frac_conserved_hit' => ['13', 'hsp', 'frac_conserved/hit', '.2f', 'FR_CNH'], 'length_aln_query' => ['14', 'hsp', 'length/query', 'd', 'LN_ALQ'], 'length_aln_hit' => ['15', 'hsp', 'length/hit', 'd', 'LN_ALH'], 'gaps_query' => ['16', 'hsp', 'gaps/query', 'd', 'GAPS_Q'], 'gaps_hit' => ['17', 'hsp', 'gaps/hit', 'd', 'GAPS_H'], 'gaps_total' => ['18', 'hsp', 'gaps/total', 'd', 'GAPS_QH'], 'start_query' => ['19', 'hsp', 'start/query', 'd', 'START_Q'], 'end_query' => ['20', 'hsp', 'end/query', 'd', 'END_Q'], 'start_hit' => ['21', 'hsp', 'start/hit', 'd', 'START_H'], 'end_hit' => ['22', 'hsp', 'end/hit', 'd', 'END_H'], 'strand_query' => ['23', 'hsp', 'strand/query', 'd', 'STRND_Q'], 'strand_hit' => ['24', 'hsp', 'strand/hit', 'd', 'STRND_H'], 'frame' => ['25', 'hsp', 'frame', 's', 'FRAME'], 'hit_description' => ['26', 'hit', 'hit_description', 's', 'DESC_H'], 'query_description' => ['27', 'result', 'query_description', 's', 'DESC_Q'], )
Included modules
Bio::SearchIO::Writer::ResultTableWriter
Inherit
Bio::SearchIO::Writer::ResultTableWriter
Synopsis
    use Bio::SearchIO;
    use Bio::SearchIO::Writer::HSPTableWriter;

    my $in = Bio::SearchIO->new();

    my $writer = Bio::SearchIO::Writer::HSPTableWriter->new();

    my $out = Bio::SearchIO->new( -writer => $writer );

    while ( my $result = $in->next_result() ) {
        $out->write_result($result, ($in->report_count - 1 ? 0 : 1) );
    }
    use Bio::SearchIO;
    use Bio::SearchIO::Writer::HSPTableWriter;

    my $in = Bio::SearchIO->new();

    my $writer = Bio::SearchIO::Writer::HSPTableWriter->new( 
                                  -columns => [qw(
                                                  query_name
                                                  query_length
                                                  hit_name
                                                  hit_length
                                                  rank
                                                  frac_identical_query
                                                  expect
                                                  )]  );

    my $out = Bio::SearchIO->new( -writer => $writer,
				  -file   => ">searchio.out" );

    while ( my $result = $in->next_result() ) {
        $out->write_result($result, ($in->report_count - 1 ? 0 : 1) );
    }
You can also specify different column labels if you don't want to use
the defaults. Do this by specifying a -labels hash reference
parameter when creating the HSPTableWriter object. The keys of the
hash should be the column number (left-most column = 1) for the label(s)
you want to specify. Here's an example:
    my $writer = Bio::SearchIO::Writer::HSPTableWriter->new( 
                               -columns => [qw( query_name 
                                                query_length
                                                hit_name
                                                hit_length  )],
                               -labels  => { 1 => 'QUERY_GI',
  	                                     3 => 'HIT_IDENTIFIER' } );
Description
Bio::SearchIO::Writer::HSPTableWriter generates output at the finest
level of granularity for data within a search result. Data for each HSP
within each hit in a search result is output in tab-delimited format,
one row per HSP. Here are the columns that can be specified in the -columns
parameter when creating a HSPTableWriter object. If a -columns parameter
is not specified, this list, in this order, will be used as the default.
    query_name             # Sequence identifier of the query.
    query_length           # Full length of the query sequence
    hit_name               # Sequence identifier of the hit
    hit_length             # Full length of the hit sequence
    round                  # Round number for hit (PSI-BLAST)
    rank
    expect                 # Expect value for the alignment
    score                  # Score for the alignment (e.g., BLAST score)
    bits                   # Bit score for the alignment
    frac_identical_query   # fraction of identical substitutions in query
    frac_identical_hit     # fraction of identical substitutions in hit
    frac_conserved_query   # fraction of conserved substitutions in query
    frac_conserved_hit     # fraction of conserved substitutions in hit
    length_aln_query       # Length of the aligned portion of the query sequence
    length_aln_hit         # Length of the aligned portion of the hit sequence
    gaps_query             # Number of gaps in the aligned query sequence
    gaps_hit               # Number of gaps in the aligned hit sequence
    gaps_total             # Number of gaps in the aligned query and hit sequences
    start_query            # Starting coordinate of the aligned portion of the query sequence
    end_query              # Ending coordinate of the aligned portion of the query sequence
    start_hit              # Starting coordinate of the aligned portion of the hit sequence
    end_hit                # Ending coordinate of the aligned portion of the hit sequence
    strand_query           # Strand of the aligned query sequence
    strand_hit             # Strand of the aligned hit sequence
    frame                  # Reading frame of the aligned query sequence 
    hit_description        # Full description of the hit sequence
    query_description      # Full description of the query sequence
For more details about these columns, see the documentation for the
corresponding method in Bio::Search::HSP::HSPI.
Methods
column_map
No description
Code
end_reportDescriptionCode
to_stringDescriptionCode
Methods description
end_reportcode    nextTop
 Title   : end_report
 Usage   : $self->end_report()
 Function: The method to call when ending a report, this is
           mostly for cleanup for formats which require you to 
           have something at the end of the document.  Nothing for
           a text message.
 Returns : string
 Args    : none
to_string()codeprevnextTop
Note: this method is not intended for direct use.
The SearchIO::write_result() method calls it automatically
if the writer is hooked up to a SearchIO object as illustrated in
the SYNOPSIS section .
 Title     : to_string()
           :
 Usage     : print $writer->to_string( $result_obj, [$include_labels] );
           :
 Argument  : $result_obj = A Bio::Search::Result::ResultI object
           : $include_labels = boolean, if true column labels are included (default: false)
           :
 Returns   : String containing tab-delimited set of data for each HSP
           : in each Hit of the supplied ResultI object. 
           :
 Throws    : n/a
Methods code
column_mapdescriptionprevnextTop
sub column_map {
 return %column_map
}
end_reportdescriptionprevnextTop
sub end_report {
    return '';
}
to_stringdescriptionprevnextTop
sub to_string {
    my ($self, $result, $include_labels) = @_;
    
    my $str = $include_labels ? $self->column_labels() : '';
    my ($resultfilter,$hitfilter,
	$hspfilter) = ( $self->filter('RESULT'),
			$self->filter('HIT'),
			$self->filter('HSP'));
    if( ! defined $resultfilter || &{$resultfilter}($result) ) {
	my $func_ref = $self->row_data_func;
	my $printf_fmt = $self->printf_fmt;
	$result->can('rewind') && 
	    $result->rewind(); # insure we're at the beginning
	while( my $hit = $result->next_hit) {
	    next if( defined $hitfilter && ! &{$hitfilter}($hit) );
	    $hit->can('rewind') && $hit->rewind;# insure we're at the beginning
	    while(my $hsp = $hit->next_hsp) {
		next if ( defined $hspfilter && ! &{$hspfilter}($hsp));
		my @row_data  = &{$func_ref}($result, $hit, $hsp);
		$str .= sprintf "$printf_fmt\n", @row_data;
	    }
	}
    }
    $str =~ s/\t\n/\n/gs;
    return $str;
}
General documentation
TODOTop
Figure out the best way to incorporate algorithm-specific score columns.
The best route is probably to have algorith-specific subclasses
(e.g., BlastHSPTableWriter, FastaHSPTableWriter).
FEEDBACKTop
Mailing Lists Top
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
    bioperl-l@bioperl.org              - General discussion
    http://bio.perl.org/MailList.html  - About the mailing lists
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via email
or the web:
    bioperl-bugs@bio.perl.org                   
    http://bugzilla.bioperl.org/
AUTHOR Top
Steve Chervitz <sac@bioperl.org>
See the FEEDBACK section for where to send bug reports
and comments.
COPYRIGHTTop
Copyright (c) 2001 Steve Chervitz. All Rights Reserved.
This library is free software; you can redistribute it and/or modify
it under the same terms as Perl itself.
DISCLAIMERTop
This software is provided "as is" without warranty of any kind.
SEE ALSOTop
    Bio::SearchIO::Writer::HitTableWriter
    Bio::SearchIO::Writer::ResultTableWriter
filterTop
 Title   : filter
 Usage   : $writer->filter('hsp', \&hsp_filter);
 Function: Filter out either at HSP,Hit,or Result level
 Returns : none
 Args    : string => data type,
           CODE reference