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Summary

Bio::SeqIO::ace - ace sequence input/output stream

Package variables

Included modules

Inherit

Bio::SeqIO

Synopsis

Do not use this module directly. Use it via the Bio::SeqIO class.

Description

This object can transform Bio::Seq objects to and
from ace file format. It only parses a DNA or
Peptide objects contained in the ace file,
producing PrimarySeq objects from them. All
other objects in the files will be ignored. It
doesn't attempt to parse any annotation attatched
to the containing Sequence or Protein objects,
which would probably be impossible, since
everyone's ACeDB schema can be different.
It won't parse ace files containing Timestamps
correctly either. This can easily be added if
considered necessary.

Methods

Methods description

 Title   : next_seq
 Usage   : $seq = $stream->next_seq()
 Function: returns the next sequence in the stream
 Returns : Bio::Seq object
 Args    : NONE

 Title   : write_seq
 Usage   : $stream->write_seq(@seq)
 Function: writes the $seq object into the stream
 Returns : 1 for success and 0 for error
 Args    : Bio::Seq object(s)

Methods code

sub _initialize {

  my($self,@args) = @_;
  $self->SUPER::_initialize(@args);   
  if( ! defined $self->sequence_factory ) {
      $self->sequence_factory(new Bio::Seq::SeqFactory(-verbose => $self->verbose(), -type => 'Bio::PrimarySeq'));      
  }

}

sub next_seq {

        my( $self ) = @_;
        local $/ = "";  # Split input on blank lines

        my $fh = $self->_filehandle;
        my( $type, $id );
        while (<$fh>) {
            if (($type, $id) = /^(DNA|Peptide)[\s:]+(.+?)\s*\n/si) {
                s/^.+$//m;  # Remove first line
                s/\s+//g;   # Remove whitespace
                last;
            }
        }
        # Return if there weren't any DNA or peptide objects
        return unless $type;
        
        # Choose the molecule type
        my $mol_type = $bio_mol_type{lc $type}
            or $self->throw("Can't get Bio::Seq molecule type for '$type'");

        # Remove quotes from $id
        $id =~ s/^"|"$//g;
        
        # Un-escape forward slashes, double quotes, percent signs,
        # semi-colons, tabs, and backslashes (if you're mad enough
        # to have any of these as part of object names in your acedb
        # database).
	$id =~ s/\\([\/"%;\t\\])/$1/g;
#"
	# Called as next_seq(), so give back a Bio::Seq
	return $self->sequence_factory->create(
					       -seq        => $_,
					       -primary_id => $id,
					       -display_id => $id,
					       -alphabet    => $mol_type,
					       );        
    }

}

sub write_seq {

    my ($self, @seq) = @_;
    
    foreach my $seq (@seq) {
	$self->throw("Did not provide a valid Bio::PrimarySeqI object") 
	    unless defined $seq && ref($seq) && $seq->isa('Bio::PrimarySeqI');
        my $mol_type = $seq->alphabet;
        my $id = $seq->display_id;
        
        # Escape special charachers in id
        $id =~ s/([\/"%;\t\\])/\\$1/g;
#"        
        # Print header for DNA or Protein object
        if ($mol_type eq 'dna') {
            $self->_print( 
                qq{\nSequence : "$id"\nDNA "$id"\n},
                qq{\nDNA : "$id"\n},
            );
        }
        elsif ($mol_type eq 'protein') {
            $self->_print(
                qq{\nProtein : "$id"\nPeptide "$id"\n},
                qq{\nPeptide : "$id"\n},
            );
        }
        else {
            $self->throw("Don't know how to produce ACeDB output for '$mol_type'");
        }

        # Print the sequence
        my $str = $seq->seq;
        my( $formatted_seq );
        while ($str =~ /(.{1,60})/g) {
            $formatted_seq .= "$1\n";
        }
        $self->_print($formatted_seq, "\n");
    }

    $self->flush if $self->_flush_on_write && defined $self->_fh;
    return 1;
}

1;

}

General documentation

User feedback is an integral part of the evolution of this
and other Bioperl modules. Send your comments and suggestions preferably
to one of the Bioperl mailing lists.
Your participation is much appreciated.

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Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution.
Bug reports can be submitted via email or the web:

  bioperl-bugs@bio.perl.org
  http://bugzilla.bioperl.org/

Email: jgrg@sanger.ac.uk

The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _