Bio::SeqIO bsml
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Summary
Bio::SeqIO::bsml - BSML sequence input/output stream
Package variables
Privates (from "my" definitions)
$idcounter = {}
$nvtoken = ": "
Included modules
Bio::Annotation::Collection
Bio::Annotation::Comment
Bio::Annotation::DBLink
Bio::Annotation::Reference
Bio::Seq::SeqFactory
Bio::SeqFeature::Generic
Bio::SeqIO
Bio::Species
XML::DOM
Inherit
Bio::SeqIO
Synopsis
 It is probably best not to use this object directly, but rather go
through the SeqIO handler system. To read a BSML file:
$stream = Bio::SeqIO->new( -file => $filename, -format => 'bsml'); while ( my $bioSeqObj = $stream->next_seq() ) { # do something with $bioSeqObj } To write a Seq object to the current file handle in BSML XML format: $stream->write_seq( -seq => $seqObj); If instead you would like a XML::DOM object containing the BSML, use: my $newXmlObject = $stream->to_bsml( -seq => $seqObj);
Description
 This object can transform Bio::Seq objects to and from BSML (XML)
flatfiles.
 2/1/02 - I have changed the API to more closely match argument
passing used by other BioPerl methods ( -tag => value ). Internal
methods are using the same API, but you should not be calling those
anyway...
Methods
DESTROY
No description
Code
FIRSTDATA
No description
Code
FLOPPYVALS
No description
Code
GETFLOPPIES
No description
Code
STRIP
No description
Code
_add_pageDescriptionCode
_addelDescriptionCode
_initializeDescriptionCode
_parse_annotationDescriptionCode
_parse_annotation_oldDescriptionCode
_parse_bsml_featureDescriptionCode
_parse_bsml_locationDescriptionCode
_parse_locationDescriptionCode
_parse_referenceDescriptionCode
_parse_xmlDescriptionCode
_parseparamsDescriptionCode
_show_dnaDescriptionCode
next_seqDescriptionCode
to_bsmlDescriptionCode
write_seqDescriptionCode
Methods description
_add_pagecode    nextTop
 Title   : _add_page
Usage : $obj->_add_page($xmlDocument, $xmlSequenceObject)
Function: Adds a simple <Page> and <View> structure for a <Sequence>
Returns : a reference to the newly created <Page>
Args : 0 The DOM::Document being modified
1 Reference to the <Sequence> object
_addelcodeprevnextTop
 Title   : _addel
Usage : $obj->_addel($parentElem, 'ChildName',
{ anAttr => 'someValue', anotherAttr => 'aValue',})
Function: Add an element with attribute values to a DOM tree
Returns : a reference to the newly added element
Args : 0 The DOM::Element parent that you want to add to
1 The name of the new child element
2 Optional hash reference containing
attribute name => attribute value assignments
_initializecodeprevnextTop
 Title   : _initialize
Usage : $dom = $obj->_initialize(@args)
Function: Coppied from embl.pm, and augmented with initialization of the
XML DOM tree
Returns :
Args : -file => the XML file to be parsed
_parse_annotationcodeprevnextTop
 Title   : _parse_annotation
Usage : $obj->_parse_annotation(@args )
Function: Will examine any Annotations found in -obj. Data found in
::Comment and ::DBLink structures, as well as Annotation
description fields are stored in -desc for later
generation of <Attribute>s. <Reference> objects are generated
from ::References, and are stored in -refs - these will
be appended to the XML tree later.
Returns :
Args : Argument array. Recognized keys:
-xml The DOM::Document being modified -obj Reference to the Bio object being analyzed -descr An array reference for holding description text items -refs An array reference to hold DOM objects -id Optional. If the XML id for the 'calling' element is provided, it will be placed in any refs attribute.
_parse_annotation_oldcodeprevnextTop
    Title   : _parse_annotation_old
Usage : $obj->_parse_annotation_old(@args)
Function: As above, but for the old Annotation system.
Apparently needed because Features are still using the old-style
annotations?
Returns :
Args : Argument array. Recognized keys:
-xml The DOM::Document being modified -obj Reference to the Bio object being analyzed -descr An array reference for holding description text items -refs An array reference to hold DOM objects -id Optional. If the XML id for the 'calling' element is provided, it will be placed in any refs attribute.
_parse_bsml_featurecodeprevnextTop
 Title   : _parse_bsml_feature
Usage : $obj->_parse_bsml_feature($xmlFeature )
Function: Will examine the <Feature> element provided by $xmlFeature and
return a generic seq feature.
Returns : Bio::SeqFeature::Generic
Args : 0 XML::DOM::Element <Feature> being analyzed.
_parse_bsml_locationcodeprevnextTop
 Title   : _parse_bsml_location
Usage : $obj->_parse_bsml_feature( $intOrSiteLoc, $gsfObject )
Function: Will examine the <Interval-loc> or <Site-loc> element provided
Returns : Bio::SeqFeature::Generic
Args : 0 XML::DOM::Element <Interval/Site-loc> being analyzed.
1 Optional SeqFeature::Generic to use
_parse_locationcodeprevnextTop
 Title   : _parse_location
Usage : $obj->_parse_location($xmlDocument, $parentElem, $SeqFeatureObj)
Function: Adds <Interval-loc> and <Site-loc> children to <$parentElem> based
on locations / sublocations found in $SeqFeatureObj. If
sublocations exist, the original location will be ignored.
Returns : An array ref containing the elements added to the parent.
These will have already been added to <$parentElem>
Args : 0 The DOM::Document being modified
1 The DOM::Element parent that you want to add to
2 Reference to the Bio::SeqFeature being analyzed
_parse_referencecodeprevnextTop
 Title   : _parse_reference
Usage : $obj->_parse_reference(@args )
Function: Makes a new <Reference> object from a ::Reference, which is
then stored in an array provide by -refs. It will be
appended to the XML tree later.
Returns :
Args : Argument array. Recognized keys:
-xml The DOM::Document being modified -refobj The Annotation::Reference Object -refs An array reference to hold the new DOM object -id Optional. If the XML id for the 'calling' element is provided, it will be placed in any refs attribute.
_parse_xmlcodeprevnextTop
 Title   : _parse_xml
Usage : $dom = $obj->_parse_xml($filename)
Function: uses XML::DOM to construct a DOM tree from the BSML document
Returns : a reference to the parsed DOM tree
Args : 0 Path to the XML file needing to be parsed
_parseparamscodeprevnextTop
 Title   : _parseparams
Usage : my $paramHash = $obj->_parseparams(@args)
Function: Borrowed from Bio::Parse.pm, who borrowed it from CGI.pm
Lincoln Stein -> Richard Resnick -> here
Returns : A hash reference of the parameter keys (uppercase) pointing to
their values.
Args : An array of key, value pairs. Easiest to pass values as:
-key1 => value1, -key2 => value2, etc
Leading "-" are removed.
_show_dnacodeprevnextTop
 Title   : _show_dna
Usage : $obj->_show_dna($newval)
Function: (cut-and-pasted directly from embl.pm)
Returns : value of _show_dna
Args : newvalue (optional)
next_seqcodeprevnextTop
 Title   : next_seq
Usage : my $bioSeqObj = $stream->next_seq
Function: Retrieves the next sequence from a SeqIO::bsml stream.
Returns : A reference to a Bio::Seq::RichSeq object
Args :
to_bsmlcodeprevnextTop
 Title   : to_bsml
Usage : my $domDoc = $obj->to_bsml(@args)
Function: Generates an XML structure for one or more Bio::Seq objects.
If $seqref is an array ref, the XML tree generated will include
all the sequences in the array.
Returns : A reference to the XML DOM::Document object generated / modified
Args : Argument array in form of -key => val. Recognized keys:
-seq A Bio::Seq reference, or an array reference of many of them -xmldoc Specifies an existing XML DOM document to add the sequences to. If included, then only data (no page formatting) will be added. If not, a new XML::DOM::Document will be made, and will be populated with both data, as well as display elements. -nodisp Do not generate elements, or any children thereof, even if -xmldoc is not set. -skipfeat If set to 'all', all s will be skipped. If it is a hash reference, any with a class matching a key in the hash will be skipped - for example, to skip 'source' and 'score' features, use: -skipfeat => { source => 'Y', score => 'Y' } -skiptags As above: if set to 'all', no tags are included, and if a hash reference, those specific tags will be ignored. Skipping some or all tags and features can result in noticable speed improvements. -nodata If true, then will not be included. This may be useful if you just want annotations and do not care about the raw ACTG information. -return Default is 'xml', which will return a reference to the BSML XML object. If set to 'seq' will return an array ref of the objects added (rather than the whole XML object) -close Early BSML browsers will crash if an element *could* have children but does not, and is closed as an empty element e.g. . If -close is true, then such tags are given a comment child to explicitly close them e.g. . This is default true, set to "0" if you do not want this behavior. Examples : my $domObj = $stream->to_bsml( -seq => \@fourCoolSequenceObjects, -skipfeat => { source => 1 }, ); # Or add sequences to an existing BSML document: $stream->to_bsml( -seq => \@fourCoolSequenceObjects, -skipfeat => { source => 1 }, -xmldoc => $myBsmlDocumentInProgress, );
write_seqcodeprevnextTop
 Title   : write_seq
Usage : $obj->write_seq(@args)
Function: Prints out an XML structure for one or more Bio::Seq objects.
If $seqref is an array ref, the XML tree generated will include
all the sequences in the array. This method is fairly simple,
most of the processing is performed within to_bsml.
Returns : A reference to the XML object generated / modified
Args : Argument array. Recognized keys:
-seq A Bio::Seq reference, or an array reference of many of them Alternatively, the method may be called simply as... $obj->write_seq( $bioseq ) ... if only a single argument is passed, it is assumed that it is the sequence object (can also be an array ref of many Seq objects )
-printmime If true prints "Content-type: $mimetype\n\n" at top of
document, where $mimetype is the value designated by this
key. For generic XML use text/xml, for BSML use text/x-bsml
   -return This option will be supressed, since the nature of this
method is to print out the XML document. If you wish to
retrieve the <Sequence> objects generated, use the to_bsml
method directly.
Methods code
DESTROYdescriptionprevnextTop
sub DESTROY {
    my $self = shift;
    # Reports off the net imply that DOM::Parser will memory leak if you
# do not explicitly dispose of it:
# http://aspn.activestate.com/ASPN/Mail/Message/perl-xml/788458
my $dom = $self->{'domtree'}; # For some reason the domtree can get undef-ed somewhere...
$dom->dispose if ($dom);
}
FIRSTDATAdescriptionprevnextTop
sub FIRSTDATA {
    my $element = shift;
    return undef unless ($element);

    my $hopefuls = $element->getChildNodes;
    my $data;
    for (my $i = 0; $i < $hopefuls->getLength; $i++) {
	if ($hopefuls->item($i)->getNodeType ==
	  XML::DOM::Node::TEXT_NODE() ) {
	    $data = $hopefuls->item($i)->getNodeValue;
	    last;
	}
    }
    return $data;
}
#- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 
# Just collapses whitespace runs in a string
}
FLOPPYVALSdescriptionprevnextTop
sub FLOPPYVALS {
    my $obj = shift;

    my ($name, $value);
    if      ($obj->getNodeName eq "Attribute") {
	$name  = $obj->getAttribute('name');
	$value = $obj->getAttribute('content');
    } elsif ($obj->getNodeName eq "Qualifier") {
	# Wheras <Attribute>s require both 'name' and 'content' attributes,
# <Qualifier>s can technically have either blank (and sometimes do)
my $n = $obj->getAttribute('value-type'); $name = $n if ($n ne ""); my $v = $obj->getAttribute('value'); $value = $v if ($v ne ""); } return ($name, $value); } #- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Returns the value of the first TEXT_NODE encountered below an element
# Rational - avoid grabbing a comment rather than the PCDATA. Not foolproof...
}
GETFLOPPIESdescriptionprevnextTop
sub GETFLOPPIES {
    my $obj = shift;
    
    my @floppies;
    my $attributes = $obj->getElementsByTagName ("Attribute");
    for (my $i = 0; $i < $attributes->getLength; $i++) {
	push @floppies, $attributes->item($i);
    }
    my $qualifiers = $obj->getElementsByTagName ("Qualifier");
    for (my $i = 0; $i < $qualifiers->getLength; $i++) {
	push @floppies, $qualifiers->item($i);
    }
    return\@ floppies;
}
#- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 
# Given a DOM <Attribute> or <Qualifier> object, return the [name, value] pair
}
STRIPdescriptionprevnextTop
sub STRIP {
    my $string = shift;
    $string =~ s/[\s\r\n]+/ /g;
    return $string;
}
_add_pagedescriptionprevnextTop
sub _add_page {
    my $self = shift;
    my ($xml, $seq) = @_;
    my $disp = $xml->getElementsByTagName("Display")->item(0);
    my $page = $self->_addel($disp, "Page");
    my ($width, $height) = ( 7.8, 5.5);
    my $screen = $self->_addel($page, "Screen", {
	width => $width, height => $height, });
#    $screen->appendChild($xml->createComment("Must close explicitly"));
my $view = $self->_addel($page, "View", { seqref => $seq->getAttribute('id'), title => $seq->getAttribute('title'), title1 => "{NAME}", title2 => "{LENGTH} {UNIT}", }); $self->_addel($view, "View-line-widget", { shape => 'horizontal', hcenter => $width/2 + 0.7,
'linear-length' =>
$width - 2,
});
$self->_addel($view, "View-axis-widget"); return $page;
}
_addeldescriptionprevnextTop
sub _addel {
    my $self = shift;
    my ($root, $name, $attr) = @_;

    # Find the DOM::Document for the parent
my $doc = $root->getOwnerDocument || $root; my $elem = $doc->createElement($name); foreach my $a (keys %{$attr}) { $elem->setAttribute($a, $attr->{$a}); } $root->appendChild($elem); return $elem;
}
_initializedescriptionprevnextTop
sub _initialize {
  my($self,@args) = @_;

  $self->SUPER::_initialize(@args);  
  # hash for functions for decoding keys.
$self->{'_func_ftunit_hash'} = {}; $self->_show_dna(1); # sets this to one by default. People can change it
my %param = @args; # From SeqIO.pm
@param{ map { lc $_ } keys %param } = values %param; # lowercase keys
if ( exists $param{-file} && $param{-file} !~ /^>/) { # Is it blasphemy to add your own keys to an object in another package?
# domtree => the parsed DOM tree retruned by XML::DOM
$self->{'domtree'} = $self->_parse_xml( $param{-file} ); # current_node => the <Sequence> node next in line for next_seq
$self->{'current_node'} = 0; } $self->sequence_factory( new Bio::Seq::SeqFactory ( -verbose => $self->verbose(), -type => 'Bio::Seq::RichSeq')) if( ! defined $self->sequence_factory );
}
_parse_annotationdescriptionprevnextTop
sub _parse_annotation {
    my $self = shift;
    my $args = $self->_parseparams( @_);
    my ($xml, $obj, $descRef, $refRef) = 
	( $args->{XML}, $args->{OBJ}, $args->{DESC}, $args->{REFS} );
    # No good place to put any of this (except for references). Most stuff
# just gets dumped to <Attribute>s
my $ann = $obj->annotation; return undef unless ($ann); # use BMS::Branch; my $debug = BMS::Branch->new( ); warn "$obj :"; $debug->branch($ann);
unless (ref($ann) =~ /Collection/) { # Old style annotation. It seems that Features still use this
# form of object
$self->_parse_annotation_old(@_); return; } foreach my $key ($ann->get_all_annotation_keys()) { foreach my $thing ($ann->get_Annotations($key)) { if ($key eq 'description') { push @{$descRef}, ["description" , $thing->value]; } elsif ($key eq 'comment') { push @{$descRef}, ["comment" , $thing->text]; } elsif ($key eq 'dblink') { # DBLinks get dumped to attributes, too
push @{$descRef}, ["db_xref" , $thing->database . ":" . $thing->primary_id ]; if (my $com = $thing->comment) { push @{$descRef}, ["link" , $com->text ]; } } elsif ($key eq 'reference') { $self->_parse_reference( @_, -refobj => $thing ); } elsif (ref($thing) =~ /SimpleValue/) { push @{$descRef}, [$key , $thing->value]; } else { # What is this??
push @{$descRef}, ["error", "bsml.pm did not understand ". "'$key' = '$thing'" ]; } } }
}
_parse_annotation_olddescriptionprevnextTop
sub _parse_annotation_old {
    my $self = shift;
    my $args = $self->_parseparams( @_);
    my ($xml, $obj, $descRef, $refRef) = 
	( $args->{XML}, $args->{OBJ}, $args->{DESC}, $args->{REFS} );
    # No good place to put any of this (except for references). Most stuff
# just gets dumped to <Attribute>s
if (my $ann = $obj->annotation) { push @{$descRef}, ["annotation", $ann->description]; foreach my $com ($ann->each_Comment) { push @{$descRef}, ["comment" , $com->text]; } # Gene names just get dumped to <Attribute name="gene">
foreach my $gene ($ann->each_gene_name) { push @{$descRef}, ["gene" , $gene]; } # DBLinks get dumped to attributes, too
foreach my $link ($ann->each_DBLink) { push @{$descRef}, ["db_xref" , $link->database . ":" . $link->primary_id ]; if (my $com = $link->comment) { push @{$descRef}, ["link" , $com->text ]; } } # References get produced and temporarily held
foreach my $ref ($ann->each_Reference) { $self->_parse_reference( @_, -refobj => $ref ); } }
}
_parse_bsml_featuredescriptionprevnextTop
sub _parse_bsml_feature {
    my $self = shift;
    my ($feat) = @_;
    
    my $basegsf = new Bio::SeqFeature::Generic;
       # score
# frame
# source_tag
# Use the class as the primary tag value, if it is present
if ( my $val = $feat->getAttribute("class") ) { $basegsf->primary_tag($val); } # Positional information is in <Interval-loc>s or <Site-loc>s
# We need to grab these in order, to try to recreate joins...
my @locations = (); foreach my $kid ($feat->getChildNodes) { my $nodeName = $kid->getNodeName; next unless ($nodeName eq "Interval-loc" || $nodeName eq "Site-loc"); push @locations, $kid; } if ($#locations == 0) { # There is only one location specified
$self->_parse_bsml_location($locations[0], $basegsf); } elsif ($#locations > 0) { #>>>> # This is not working, I think the error is somewhere downstream
# of add_sub_SeqFeature, probably in RangeI::union ?
# The sub features are added fine, but the EXPANDed parent feature
# location has a messed up start - Bio::SeqFeature::Generic ref
# instead of an integer - and an incorrect end - the end of the first
# sub feature added, not of the union of all of them.
# Also, the SeqIO::genbank.pm output is odd - the sub features appear
# to be listed with the *previous* feature, not this one.
foreach my $location (@locations) { my $subgsf = $self->_parse_bsml_location($location); # print "start ", $subgsf->start,"\n";
# print "end ", $subgsf->end,"\n";
$basegsf->add_sub_SeqFeature($subgsf, 'EXPAND'); } # print $feat->getAttribute('id'),"\n";
# print $basegsf->primary_tag,"\n";
} else { # What to do if there are no locations? Nothing needed?
} # Look at any <Attribute>s or <Qualifier>s that are present:
my $floppies = &GETFLOPPIES($feat); foreach my $attr (@{$floppies}) { my ($name, $content) = &FLOPPYVALS($attr); if ($name =~ /xref/i) { # Do we want to put these in DBLinks??
} # Don't know what the object is, dump it to a tag:
$basegsf->add_tag_value(lc($name), $content); } # Mostly this helps with debugging, but may be of utility...
# Add a tag holding the BSML id value
if ( (my $val = $feat->getAttribute('id')) && !$basegsf->has_tag('bsml-id')) { # Decided that this got a little sloppy...
# $basegsf->add_tag_value("bsml-id", $val);
} return $basegsf;
}
_parse_bsml_locationdescriptionprevnextTop
sub _parse_bsml_location {
    my $self = shift;
    my ($loc, $gsf) = @_;
    
    $gsf ||= new Bio::SeqFeature::Generic;
    my $type = $loc->getNodeName;
    my ($start, $end);
    if ($type eq 'Interval-loc') {
	$start = $loc->getAttribute('startpos');
	$end = $loc->getAttribute('endpos');
    } elsif ($type eq 'Site-loc') {
	$start = $end = $loc->getAttribute('sitepos');
    } else {
	warn "Unknown location type '$type', could not make GSF\n";
	return undef;
    }
    $gsf->start($start);
    $gsf->end($end);

    # BSML does not have an explicit method to set undefined strand
if (my $s = $loc->getAttribute("complement")) { if ($s) { $gsf->strand(-1); } else { $gsf->strand(1); } } else { # We're setting "strand nonspecific" here - bad idea?
# In most cases the user likely meant it to be on the + strand
$gsf->strand(0); } return $gsf;
}
_parse_locationdescriptionprevnextTop
sub _parse_location {
    my $self = shift;
    my ($xml, $xmlFeat, $bioFeat) = @_;
    my $bioLoc = $bioFeat->location;
    my @locations;
    if (ref($bioLoc) =~ /Split/) {
	@locations = $bioLoc->sub_Location;
	# BSML 2.2 does not recognize / support joins. For this reason,
# we will just use the upper-level location. The line below can
# be deleted or commented out if/when BSML 3 supports complex
# interval deffinitions:
@locations = ($bioLoc); } else { @locations = ($bioLoc); } my @added = (); # Add the site or interval positional information:
foreach my $loc (@locations) { my ($start, $end) = ($loc->start, $loc->end); my %locAttr; # Strand information is not well described in BSML
$locAttr{complement} = 1 if ($loc->strand == -1); if ($start ne "" && ($start == $end || $end eq "")) { $locAttr{sitepos} = $start; push @added, $self->_addel($xmlFeat,'Site-loc',\%locAttr); } elsif ($start ne "" && $end ne "") { if ($start > $end) { # The feature is on the complementary strand
($start, $end) = ($end, $start); $locAttr{complement} = 1; } $locAttr{startpos} = $start; $locAttr{endpos} = $end; push @added, $self->_addel($xmlFeat,'Interval-loc',\%locAttr); } else { warn "Failure to parse SeqFeature location. Start = '$start' & End = '$end'"; } } return\@ added;
}
_parse_referencedescriptionprevnextTop
sub _parse_reference {
    my $self = shift;
    my $args = $self->_parseparams( @_);
    my ($xml, $ref, $refRef) = ($args->{XML}, $args->{REFOBJ}, $args->{REFS});

    ###############
# <Reference> #
###############
my $xmlRef = $xml->createElement("Reference"); #>> This may not be the right way to make a BSML dbxref...
if (my $link = $ref->medline) { $xmlRef->setAttribute('dbxref', $link); } # Make attributes for some of the characteristics
my %stuff = ( start => $ref->start, end => $ref->end, rp => $ref->rp, comment => $ref->comment, pubmed => $ref->pubmed, ); foreach my $s (keys %stuff) { $self->_addel($xmlRef, "Attribute", { name => $s, content => $stuff{$s} }) if ($stuff{$s}); } $xmlRef->setAttribute('refs', $args->{ID}) if ($args->{ID}); # Add the basic information
# Should probably check for content before creation...
$self->_addel($xmlRef, "RefAuthors")-> appendChild( $xml->createTextNode(&STRIP($ref->authors)) ); $self->_addel($xmlRef, "RefTitle")-> appendChild( $xml->createTextNode(&STRIP($ref->title)) ); $self->_addel($xmlRef, "RefJournal")-> appendChild( $xml->createTextNode(&STRIP($ref->location)) ); # References will be added later in a <Feature-Table>
push @{$refRef}, $xmlRef;
}
_parse_xmldescriptionprevnextTop
sub _parse_xml {
    my $self = shift;
    my $file = shift;
    
    unless (-e $file) {
	$self->throw("Could not parse non-existant XML file '$file'.");
	return undef;
    }
    my $parser = new XML::DOM::Parser;
    my $doc = $parser->parsefile ($file);
    return $doc;
}
_parseparamsdescriptionprevnextTop
sub _parseparams {
    my $self = shift;
    my %hash = ();
    my @param = @_;
    
    # Hacked out from Parse.pm
# The next few lines strip out the '-' characters which
# preceed the keys, and capitalizes them.
for (my $i=0;$i<@param;$i+=2) { $param[$i]=~s/^\-//;
$param[$i]=~tr/a-z/A-Z/;
} pop @param if @param %2; # not an even multiple
%hash = @param; return\% hash;
}
_show_dnadescriptionprevnextTop
sub _show_dna {
   my $obj = shift;
   if( @_ ) {
      my $value = shift;
      $obj->{'_show_dna'} = $value;
    }
    return $obj->{'_show_dna'};
}
next_seqdescriptionprevnextTop
sub next_seq {
    my $self = shift;
    my ($desc);
    my $bioSeq = $self->sequence_factory->create(-verbose =>$self->verbose());
    
    unless (exists $self->{'domtree'}) {
	$self->throw("A BSML document has not yet been parsed.");
	return undef;
    }
    my $dom = $self->{'domtree'};
    my $seqElements = $dom->getElementsByTagName ("Sequence");
    if ($self->{'current_node'} == $seqElements->getLength ) {
	# There are no more <Sequence>s to process
return undef; } my $xmlSeq = $seqElements->item($self->{'current_node'}); # Assume that title attribute contains the best display id
if (my $val = $xmlSeq->getAttribute( "title")) { $bioSeq->display_id($val); } # Set the molecule type
if (my $val = $xmlSeq->getAttribute( "molecule" )) { my %mol = ('dna' => 'DNA', 'rna' => 'RNA', 'aa' => 'protein'); $bioSeq->molecule($mol{ lc($val) }); } # Set the accession number
if (my $val = $xmlSeq->getAttribute( "ic-acckey" )) { $bioSeq->accession_number($val); } # Get the sequence data for the element
if (my $seqData = &FIRSTDATA($xmlSeq->getElementsByTagName("Seq-data") ->item(0) ) ) { # Sequence data exists, transfer to the Seq object
# Remove white space and CRs (not neccesary?)
$seqData =~ s/[\s\n\r]//g; $bioSeq->seq($seqData); } elsif (my $import = $xmlSeq->getElementsByTagName("Seq-dataimport") ->item(0) ) { #>>>> # What about <Seq-data-import> ??
} elsif (my $val = $xmlSeq->getAttribute("length")) { # No sequence defined, set the length directly
#>>>> # This does not appear to work - length is apparently calculated
# from the sequence. How to make a "virtual" sequence??? Such
# creatures are common in BSML...
$bioSeq->length($val); } my $species = Bio::Species->new(); my @classification = (); # Peruse the generic <Attributes> - those that are direct children of
# the <Sequence> or the <Feature-tables> element
# Sticky wicket here - data not controlled by schema, could be anything
my @seqDesc = (); my %specs = ('common_name' => 'y', 'genus' => 'y', 'species' => 'y', 'sub_species' => 'y', ); my %seqMap = ( 'add_date' => [ 'date' ], 'keywords' => [ 'keyword', ], 'seq_version' => [ 'version' ], 'division' => [ 'division' ], 'add_secondary_accession' => ['accession'], 'pid' => ['pid'], 'primary_id' => [ 'primary.id', 'primary_id' ], ); my $floppies = &GETFLOPPIES($xmlSeq); foreach my $attr (@{$floppies}) { # Don't want to get attributes from <Feature> or <Table> elements yet
my $parent = $attr->getParentNode->getNodeName; next unless($parent eq "Sequence" || $parent eq "Feature-tables"); my ($name, $content) = &FLOPPYVALS($attr); $name = lc($name); if (exists $specs{$name}) { # It looks like part of species...
$species->$name($content); next; } my $value = ""; # Cycle through the Seq methods:
foreach my $method (keys %seqMap) { # Cycle through potential matching attributes:
foreach my $match (@{$seqMap{$method}}) { # If the <Attribute> name matches one of the keys,
# set $value, unless it has already been set
$value ||= $content if ($name =~ /$match/i); } if ($value ne "") { $bioSeq->$method($value); last; } } next if ($value ne ""); if ($name =~ /^species$/i) { # Uh, it's the species designation?
if ($content =~ / /) { # Assume that a full species name has been provided
# This will screw up if the last word is the subspecies...
my @break = split " ", $content; @classification = reverse @break; } else { $classification[0] = $content; } next; } if ($name =~ /sub[_ ]?species/i) { # Should be the subspecies...
$species->sub_species( $content ); next; } if ($name =~ /classification/i) { # Should be species classification
# We will assume that there are spaces separating the terms:
my @bits = split " ", $content; # Now make sure there is not other cruft as well (eg semi-colons)
for my $i (0..$#bits) { $bits[$i] =~ /(\w+)/; $bits[$i] = $1; } $species->classification( @bits ); next; } if ($name =~ /comment/) { my $com = Bio::Annotation::Comment->new('-text' => $content); # $bioSeq->annotation->add_Comment($com);
$bioSeq->annotation->add_Annotation('comment', $com); next; } # Description line - collect all descriptions for later assembly
if ($name =~ /descr/) { push @seqDesc, $content; next; } # Ok, we have no idea what this attribute is. Dump to SimpleValue
my $simp = Bio::Annotation::SimpleValue->new( -value => $content); $bioSeq->annotation->add_Annotation($name, $simp); } unless ($#seqDesc < 0) { $bioSeq->desc( join "; ", @seqDesc); } #>>>> This should be modified so that any IDREF associated with the
# <Reference> is then used to associate the reference with the
# appropriate Feature
# Extract out <Reference>s associated with the sequence
my @refs; my %tags = ( -title => "RefTitle", -authors => "RefAuthors", -location => "RefJournal", ); foreach my $ref ( $xmlSeq->getElementsByTagName ("Reference") ) { my %refVals; foreach my $tag (keys %tags) { my $rt = &FIRSTDATA($ref->getElementsByTagName($tags{$tag}) ->item(0)); $rt =~ s/^[\s\r\n]+//; # Kill leading space
$rt =~ s/[\s\r\n]+$//; # Kill trailing space
$rt =~ s/[\s\r\n]+/ /; # Collapse internal space runs
$refVals{$tag} = $rt; } my $reference = Bio::Annotation::Reference->new( %refVals ); # Pull out any <Reference> information hidden in <Attributes>
my %refMap = ( comment => [ 'comment', 'remark' ], medline => [ 'medline', ], pubmed => [ 'pubmed' ], start => [ 'start', 'begin' ], end => [ 'stop', 'end' ], ); my @refCom = (); my $floppies = &GETFLOPPIES($ref); foreach my $attr (@{$floppies}) { my ($name, $content) = &FLOPPYVALS($attr); my $value = ""; # Cycle through the Seq methods:
foreach my $method (keys %refMap) { # Cycle through potential matching attributes:
foreach my $match (@{$refMap{$method}}) { # If the <Attribute> name matches one of the keys,
# set $value, unless it has already been set
$value ||= $content if ($name =~ /$match/i); } if ($value ne "") { my $str = '$reference->' . $method . "($value)"; eval($str); next; } } next if ($value ne ""); # Don't know what the <Attribute> is, dump it to comments:
push @refCom, $name . $nvtoken . $content; } unless ($#refCom < 0) { # Random stuff was found, tack it to the comment field
my $exist = $reference->comment; $exist .= join ", ", @refCom; $reference->comment($exist); } push @refs, $reference; } $bioSeq->annotation->add_Annotation('reference'=>$_) foreach @refs; # Extract the <Feature>s for this <Sequence>
foreach my $feat ( $xmlSeq->getElementsByTagName("Feature") ) { $bioSeq->add_SeqFeature( $self->_parse_bsml_feature($feat) ); } $species->classification( @classification ); $bioSeq->species( $species ); # $seq->annotation->add_DBLink(@links); ->
$self->{'current_node'}++; return $bioSeq; } #- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Get all the <Attribute> and <Qualifier> children for an object, and
# return them as an array reference
# ('floppy' since these elements have poor/no schema control)
}
to_bsmldescriptionprevnextTop
sub to_bsml {
    my $self = shift;
    my $args = $self->_parseparams( -close => 1,
				    -return => 'xml',
				    @_);
    $args->{NODISP} ||= $args->{NODISPLAY};
    my $seqref = $args->{SEQ};
    $seqref = (ref($seqref) eq 'ARRAY') ? $seqref : [ $seqref ];

    #############################
# Basic BSML XML Components #
#############################
my $xml; my ($bsmlElem, $defsElem, $seqsElem, $dispElem); if ($args->{XMLDOC}) { # The user has provided an existing XML DOM object
$xml = $args->{XMLDOC}; unless ($xml->isa("XML::DOM::Document")) { die ('SeqIO::bsml.pm error:\n'. 'When calling ->to_bsml( { xmldoc => $myDoc }), $myDoc\n ' . 'should be an XML::DOM::Document object, or an object that\n'. 'inherits from that class (like BsmlHelper.pm)'); } } else { # The user has not provided a new document, make one from scratch
$xml = XML::DOM::Document->new(); $xml->setXMLDecl( $xml->createXMLDecl("1.0") ); my $url = "http://www.labbook.com/dtd/bsml2_2.dtd"; my $doc = $xml->createDocumentType("Bsml",$url); $xml->setDoctype($doc); $bsmlElem = $self->_addel( $xml, 'Bsml'); $defsElem = $self->_addel( $bsmlElem, 'Definitions'); $seqsElem = $self->_addel( $defsElem, 'Sequences'); unless ($args->{NODISP}) { $dispElem = $self->_addel( $bsmlElem, 'Display'); my $stylElem = $self->_addel( $dispElem, 'Styles'); my $style = $self->_addel( $stylElem, 'Style', { type => "text/css" }); my $styleText = qq(Interval-widget { display : "1"; }\n) . qq(Feature { display-auto : "1"; }); $style->appendChild( $xml->createTextNode($styleText) ); } } # Establish fundamental BSML elements, if they do not already exist
$bsmlElem ||= $xml->getElementsByTagName("Bsml")->item(0); $defsElem ||= $xml->getElementsByTagName("Definitions")->item(0); $seqsElem ||= $xml->getElementsByTagName("Sequences")->item(0); ###############
# <Sequences> #
###############
# Map over Bio::Seq to BSML
my %mol = ('dna' => 'DNA', 'rna' => 'RNA', 'protein' => 'AA'); my @xmlSequences; foreach my $bioSeq (@{$seqref}) { my $xmlSeq = $xml->createElement("Sequence"); my $FTs = $xml->createElement("Feature-tables"); # Array references to hold <Reference> objects:
my $seqRefs = []; my $featRefs = []; # Array references to hold <Attribute> values (not objects):
my $seqDesc = []; push @{$seqDesc}, ["comment" , "This file generated to BSML 2.2 standards - joins will be collapsed to a single feature enclosing all members of the join"]; push @{$seqDesc}, ["description" , eval{$bioSeq->desc}]; foreach my $kwd ( eval{@{$bioSeq->keywords || []}} ) { push @{$seqDesc}, ["keyword" , $kwd]; } push @{$seqDesc}, ["version" , eval{$bioSeq->seq_version}]; push @{$seqDesc}, ["division" , eval{$bioSeq->division}]; push @{$seqDesc}, ["pid" , eval{$bioSeq->pid}]; # push @{$seqDesc}, ["bio_object" , ref($bioSeq)];
my $pid = eval{$bioSeq->primary_id} || ''; if( $pid ne $bioSeq ) { push @{$seqDesc}, ["primary_id" , eval{$bioSeq->primary_id}]; } foreach my $dt (eval{$bioSeq->get_dates()} ) { push @{$seqDesc}, ["date" , $dt]; } foreach my $ac (eval{$bioSeq->get_secondary_accessions()} ) { push @{$seqDesc}, ["secondary_accession" , $ac]; } # Determine the accession number and a unique identifier
my $acc = $bioSeq->accession_number eq "unknown" ? "" : $bioSeq->accession_number; my $id; my $pi = $bioSeq->primary_id; if ($pi && $pi !~ /Bio::/) { # Not sure I understand what primary_id is... It sometimes
# is a string describing a reference to a BioSeq object...
$id = "SEQ" . $bioSeq->primary_id; } else { # Nothing useful found, make a new unique ID
$id = $acc || ("SEQ-io" . $idcounter->{Sequence}++); } # print "$id->",ref($bioSeq->primary_id),"\n";
# An id field with spaces is interpreted as an idref - kill the spaces
$id =~ s/ /-/g; # Map over <Sequence> attributes
my %attr = ( 'title' => $bioSeq->display_id, 'length' => $bioSeq->length, 'ic-acckey' => $acc, 'id' => $id, 'representation' => 'raw', ); $attr{molecule} = $mol{ lc($bioSeq->molecule) } if $bioSeq->can('molecule'); foreach my $a (keys %attr) { $xmlSeq->setAttribute($a, $attr{$a}) if (defined $attr{$a} && $attr{$a} ne ""); } # Orphaned Attributes:
$xmlSeq->setAttribute('topology', 'circular') if ($bioSeq->is_circular); # <Sequence> strand, locus
$self->_add_page($xml, $xmlSeq) if ($dispElem); ################
# <Attributes> #
################
# Check for Bio::Annotations on the * <Sequence> *.
$self->_parse_annotation( -xml => $xml, -obj => $bioSeq, -desc => $seqDesc, -refs => $seqRefs); # Incorporate species data
if (ref($bioSeq->species) eq 'Bio::Species') { # Need to peer into Bio::Species ...
my @specs = ('common_name', 'genus', 'species', 'sub_species'); foreach my $sp (@specs) { next unless (my $val = $bioSeq->species()->$sp()); push @{$seqDesc}, [$sp , $val]; } push @{$seqDesc}, ['classification', (join " ", $bioSeq->species->classification) ]; # Species::binomial will return "genus species sub_species" ...
} elsif (my $val = $bioSeq->species) { # Ok, no idea what it is, just dump it in there...
push @{$seqDesc}, ["species", $val]; } # Add the description <Attribute>s for the <Sequence>
foreach my $seqD (@{$seqDesc}) { $self->_addel($xmlSeq, "Attribute", { name => $seqD->[0], content => $seqD->[1]}) if ($seqD->[1]); } # If sequence references were added, make a Feature-table for them
unless ($#{$seqRefs} < 0) { my $seqFT = $self->_addel($FTs, "Feature-table", { title => "Sequence References", }); foreach my $feat (@{$seqRefs}) { $seqFT->appendChild($feat); } } # This is the appropriate place to add <Feature-tables>
$xmlSeq->appendChild($FTs); #############
# <Feature> #
#############
#>>>> # Perhaps it is better to loop through top_Seqfeatures?...
#>>>> # ...however, BSML does not have a hierarchy for Features
if (defined $args->{SKIPFEAT} && $args->{SKIPFEAT} eq 'all') { $args->{SKIPFEAT} = { all => 1}; } foreach my $class (keys %{$args->{SKIPFEAT}}) { $args->{SKIPFEAT}{lc($class)} = $args->{SKIPFEAT}{$class}; } # Loop through all the features
my @features = $bioSeq->all_SeqFeatures(); if (@features && !$args->{SKIPFEAT}{all}) { my $ft = $self->_addel($FTs, "Feature-table", { title => "Features", }); foreach my $bioFeat (@features ) { my $featDesc = []; my $class = lc($bioFeat->primary_tag); # The user may have specified to ignore this type of feature
next if ($args->{SKIPFEAT}{$class}); my $id = "FEAT-io" . $idcounter->{Feature}++; my $xmlFeat = $self->_addel( $ft, 'Feature', { 'id' => $id, 'class' => $class , 'value-type' => $bioFeat->source_tag }); # Check for Bio::Annotations on the * <Feature> *.
$self->_parse_annotation( -xml => $xml, -obj => $bioFeat, -desc => $featDesc, -id => $id, -refs =>$featRefs, ); # Add the description stuff for the <Feature>
foreach my $de (@{$featDesc}) { $self->_addel($xmlFeat, "Attribute", { name => $de->[0], content => $de->[1]}) if ($de->[1]); } $self->_parse_location($xml, $xmlFeat, $bioFeat); # loop through the tags, add them as <Qualifiers>
next if (defined $args->{SKIPTAGS} && $args->{SKIPTAGS} =~ /all/i); # Tags can consume a lot of CPU cycles, and can often be
# rather non-informative, so -skiptags can allow total or
# selective omission of tags.
foreach my $tag ($bioFeat->all_tags()) { next if (exists $args->{SKIPTAGS}{$tag}); foreach my $val ($bioFeat->each_tag_value($tag)) { $self->_addel( $xmlFeat, 'Qualifier', { 'value-type' => $tag , 'value' => $val }); } } } } ##############
# <Seq-data> #
##############
# Add sequence data
if ( (my $data = $bioSeq->seq) && !$args->{NODATA} ) { my $d = $self->_addel($xmlSeq, 'Seq-data'); $d->appendChild( $xml->createTextNode($data) ); } # If references were added, make a Feature-table for them
unless ($#{$featRefs} < 0) { my $seqFT = $self->_addel($FTs, "Feature-table", { title => "Feature References", }); foreach my $feat (@{$featRefs}) { $seqFT->appendChild($feat); } } # Place the completed <Sequence> tree as a child of <Sequences>
$seqsElem->appendChild($xmlSeq); push @xmlSequences, $xmlSeq; } # Prevent browser crashes by explicitly closing empty elements:
if ($args->{CLOSE}) { my @problemChild = ('Sequences', 'Sequence', 'Feature-tables', 'Feature-table', 'Screen', 'View',); foreach my $kid (@problemChild) { foreach my $prob ($xml->getElementsByTagName($kid)) { unless ($prob->hasChildNodes) { $prob->appendChild( $xml->createComment(" Must close <$kid> explicitly ")); } } } } if (defined $args->{RETURN} && $args->{RETURN} =~ /seq/i) { return\@ xmlSequences; } else { return $xml; }
}
write_seqdescriptionprevnextTop
sub write_seq {
    my $self = shift;
    my $args = $self->_parseparams( @_);
    if ($#_ == 0 ) {
	# If only a single value is passed, assume it is the seq object
unshift @_, "-seq"; } # Build a BSML XML DOM object based on the sequence(s)
my $xml = $self->to_bsml( @_, -return => undef ); # Convert to a string
my $out = $xml->toString; # Print after putting a return after each element - more readable
$out =~ s/>/>\n/g; $self->_print("Content-type: " . $args->{PRINTMIME} . "\n\n") if ($args->{PRINTMIME}); $self->_print( $out ); # Return the DOM tree in case the user wants to do something with it
$self->flush if $self->_flush_on_write && defined $self->_fh; return $xml;
}
General documentation
DEPENDENCIESTop
 In addition to parts of the Bio:: hierarchy, this module uses:
XML::DOM
FEEDBACKTop
Mailing ListsTop
 User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much
appreciated.
bioperl-l@bioperl.org - General discussion http://www.bioperl.org/MailList.shtml - About the mailing lists
Reporting BugsTop
 Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution.
Bug reports can be submitted via email or the web:
bioperl-bugs@bio.perl.org http://bugzilla.bioperl.org/
Things Still to DoTop
 * The module now uses the new Collection.pm system. However,
Annotations associated with a Feature object still seem to use the
old system, so parsing with the old methods are included..
* Generate Seq objects with no sequence data but an assigned length. This appears to be an issue with Bio::Seq. It is possible (and reasonable) to make a BSML document with features but no sequence data. * Support . Do not know how commonly this is used. * Some features are awaiting implementation in later versions of BSML. These include: * Nested feature support * Complex feature (ie joins) * Unambiguity in strand (ie -1,0,1, not just 'complement' ) * More friendly dblink structures * Location.pm (or RangeI::union?) appears to have a bug when 'expand' is used. * More intelligent hunting for sequence and feature titles? It is not terribly clear where the most appropriate field is located, better grepping (eg looking for a reasonable count for spaces and numbers) may allow for titles better than "AE008041".
AUTHOR - Charles TilfordTop
Bristol-Myers Squibb Bioinformatics
Email tilfordc@bms.com
I have developed the BSML specific code for this package, but have used
code from other SeqIO packages for much of the nuts-and-bolts. In particular
I have used code from the embl.pm module either directly or as a framework
for many of the subroutines that are common to SeqIO modules.
INTERNAL METHODSTop
#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-#-
 The following methods are used for internal processing, and should probably
not be accessed by the user.
TESTING SCRIPTTop
 The following script may be used to test the conversion process. You
will need a file of the format you wish to test. The script will
convert the file to BSML, store it in /tmp/bsmltemp, read that file
into a new SeqIO stream, and write it back as the original
format. Comparison of this second file to the original input file
will allow you to track where data may be lost or corrupted. Note
that you will need to specify $readfile and $readformat.
use Bio::SeqIO; # Tests preservation of details during round-trip conversion: # $readformat -> BSML -> $readformat my $tempspot = "/tmp/bsmltemp"; # temp folder to hold generated files my $readfile = "rps4y.embl"; # The name of the file you want to test my $readformat = "embl"; # The format of the file being tested system "mkdir $tempspot" unless (-d $tempspot); # Make Seq object from the $readfile my $biostream = Bio::SeqIO->new( -file => "$readfile" ); my $seq = $biostream->next_seq(); # Write BSML from SeqObject my $bsmlout = Bio::SeqIO->new( -format => 'bsml', -file => ">$tempspot/out.bsml"); warn "\nBSML written to $tempspot/out.bsml\n"; $bsmlout->write_seq($seq); # Need to kill object for following code to work... Why is this so? $bsmlout = ""; # Make Seq object from BSML my $bsmlin = Bio::SeqIO->new( -file => "$tempspot/out.bsml", -format => 'bsml'); my $seq2 = $bsmlin->next_seq(); # Write format back from Seq Object my $genout = Bio::SeqIO->new( -format => $readformat, -file => ">$tempspot/out.$readformat"); $genout->write_seq($seq2); warn "$readformat written to $tempspot/out.$readformat\n"; # BEING LOST: # Join information (not possible in BSML 2.2) # Sequence type (??)