Raw content of Bio::SeqIO::largefasta
<![CDATA[# $Id: largefasta.pm,v 1.18 2002/12/27 19:42:32 birney Exp $
# BioPerl module for Bio::SeqIO::largefasta
#
# Cared for by Jason Stajich
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself
# _history
#
# POD documentation - main docs before the code
=head1 NAME
Bio::SeqIO::largefasta - method i/o on very large fasta sequence files
=head1 SYNOPSIS
Do not use this module directly. Use it via the Bio::SeqIO class.
=head1 DESCRIPTION
This object can transform Bio::Seq objects to and from fasta flat
file databases.
This module handles very large sequence files by using the
Bio::Seq::LargePrimarySeq module to store all the sequence data in
a file. This can be a problem if you have limited disk space on your
computer because this will effectively cause 2 copies of the sequence
file to reside on disk for the life of the
Bio::Seq::LargePrimarySeq object. The default location for this is
specified by the L<File::Spec>-E<gt>tmpdir routine which is usually /tmp
on UNIX. If a sequence file is larger than the swap space (capacity
of the /tmp dir) this could cause problems for the machine. It is
possible to set the directory where the temporary file is located by
adding the following line to your code BEFORE calling next_seq. See
L<Bio::Seq::LargePrimarySeq> for more information.
$Bio::Seq::LargePrimarySeq::DEFAULT_TEMP_DIR = 'newdir';
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/MailList.shtml - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via email
or the web:
bioperl-bugs@bio.perl.org
http://bugzilla.bioperl.org/
=head1 AUTHORS - Jason Stajich
Email: jason@bioperl.org
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::SeqIO::largefasta;
use vars qw(@ISA $FASTALINELEN);
use strict;
# Object preamble - inherits from Bio::Root::Object
use Bio::SeqIO;
use Bio::Seq::SeqFactory;
$FASTALINELEN = 60;
@ISA = qw(Bio::SeqIO);
sub _initialize {
my($self,@args) = @_;
$self->SUPER::_initialize(@args);
if( ! defined $self->sequence_factory ) {
$self->sequence_factory(new Bio::Seq::SeqFactory
(-verbose => $self->verbose(),
-type => 'Bio::Seq::LargePrimarySeq'));
}
}
=head2 next_seq
Title : next_seq
Usage : $seq = $stream->next_seq()
Function: returns the next sequence in the stream
Returns : Bio::Seq object
Args : NONE
=cut
sub next_seq {
my ($self) = @_;
# local $/ = "\n";
my $largeseq = $self->sequence_factory->create();
my ($id,$fulldesc,$entry);
my $count = 0;
my $seen = 0;
while( defined ($entry = $self->_readline) ) {
if( $seen == 1 && $entry =~ /^\s*>/ ) {
$self->_pushback($entry);
return $largeseq;
}
# if ( ($entry eq '>') || eof($self->_fh) ) { $seen = 1; next; }
if ( ($entry eq '>') ) { $seen = 1; next; }
elsif( $entry =~ /\s*>(.+?)$/ ) {
$seen = 1;
($id,$fulldesc) = ($1 =~ /^\s*(\S+)\s*(.*)$/)
or $self->warn("Can't parse fasta header");
$largeseq->display_id($id);
$largeseq->primary_id($id);
$largeseq->desc($fulldesc);
} else {
$entry =~ s/\s+//g;
$largeseq->add_sequence_as_string($entry);
}
(++$count % 1000 == 0 && $self->verbose() > 0) && print "line $count\n";
}
if( ! $seen ) { return undef; }
return $largeseq;
}
=head2 write_seq
Title : write_seq
Usage : $stream->write_seq(@seq)
Function: writes the $seq object into the stream
Returns : 1 for success and 0 for error
Args : Bio::Seq object
=cut
sub write_seq {
my ($self,@seq) = @_;
foreach my $seq (@seq) {
my $top = $seq->id();
if ($seq->can('desc') and my $desc = $seq->desc()) {
$desc =~ s/\n//g;
$top .= " $desc";
}
$self->_print (">",$top,"\n");
my $end = $seq->length();
my $start = 1;
while( $start < $end ) {
my $stop = $start + $FASTALINELEN - 1;
$stop = $end if( $stop > $end );
$self->_print($seq->subseq($start,$stop), "\n");
$start += $FASTALINELEN;
}
}
$self->flush if $self->_flush_on_write && defined $self->_fh;
return 1;
}
1;
]]>