Raw content of Bio::SeqIO::raw #----------------------------------------------------------------------------- # PACKAGE : Bio::SeqIO::raw # AUTHOR : Ewan Birney <birney@ebi.ac.uk> # CREATED : Feb 16 1999 # REVISION: $Id: raw.pm,v 1.15.2.1 2003/02/05 21:55:21 jason Exp $ # # Copyright (c) 1997-9 bioperl, Ewan Birney. All Rights Reserved. # This module is free software; you can redistribute it and/or # modify it under the same terms as Perl itself. # # _History_ # # Ewan Birney <birney@ebi.ac.uk> developed the SeqIO # schema and the first prototype modules. # # This code is based on his Bio::SeqIO::Fasta module with # the necessary minor tweaks necessary to get it to read # and write raw formatted sequences made by # chris dagdigian <dag@sonsorol.org> # # October 18, 1999 Largely rewritten by Lincoln Stein # # Copyright Ewan Birney # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::SeqIO::raw - raw sequence file input/output stream =head1 SYNOPSIS Do not use this module directly. Use it via the L<Bio::SeqIO> class. =head1 DESCRIPTION This object can transform Bio::Seq objects to and from raw flat file databases. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://www.bioperl.org/MailList.shtml - About the mailing lists =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via email or the web: bioperl-bugs@bio.perl.org http://bugzilla.bioperl.org/ =head1 AUTHORS Ewan Birney E<lt>birney@ebi.ac.ukE<gt> Lincoln Stein E<lt>lstein@cshl.orgE<gt> =head1 CONTRIBUTORS Jason Stajich E<lt>jason@bioperl.org<gt> =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::SeqIO::raw; use strict; use vars qw(@ISA); use Bio::SeqIO; use Bio::Seq::SeqFactory; @ISA = qw(Bio::SeqIO); sub _initialize { my($self,@args) = @_; $self->SUPER::_initialize(@args); if( ! defined $self->sequence_factory ) { $self->sequence_factory(new Bio::Seq::SeqFactory (-verbose => $self->verbose(), -type => 'Bio::Seq')); } } =head2 next_seq Title : next_seq Usage : $seq = $stream->next_seq() Function: returns the next sequence in the stream Returns : Bio::Seq object Args : =cut sub next_seq{ my ($self,@args) = @_; ## When its 1 sequence per line with no formatting at all, ## grabbing it should be easy :) my $nextline = $self->_readline(); if( !defined $nextline ){ return undef; } my $sequence = uc($nextline); $sequence =~ s/\W//g; return $self->sequence_factory->create(-seq => $sequence); } =head2 write_seq Title : write_seq Usage : $stream->write_seq($seq) Function: writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Array of Bio::PrimarySeqI objects =cut sub write_seq { my ($self,@seq) = @_; foreach my $seq (@seq) { $self->throw("Must provide a valid Bio::PrimarySeqI object") unless defined $seq && ref($seq) && $seq->isa('Bio::PrimarySeqI'); $self->_print($seq->seq, "\n") or return; } $self->flush if $self->_flush_on_write && defined $self->_fh; return 1; } =head2 write_qual Title : write_qual Usage : $stream->write_qual($seq) Function: writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Bio::Seq object =cut sub write_qual { my ($self,@seq) = @_; my @qual = (); foreach (@seq) { unless ($_->isa("Bio::Seq::SeqWithQuality")){ warn("You cannot write raw qualities without supplying a Bio::Seq::SeqWithQuality object! You passed a ", ref($_), "\n"); next; } @qual = @{$_->qual}; if(scalar(@qual) == 0) { $qual[0] = "\n"; } $self->_print (join " ", @qual,"\n") or return; } return 1; } 1;