Raw content of Bio::SeqIO::swiss
<![CDATA[# $Id: swiss.pm,v 1.66.2.4 2003/09/13 22:16:43 jason Exp $
#
# BioPerl module for Bio::SeqIO::swiss
#
# Cared for by Elia Stupka <elia@tll.org.sg>
#
# Copyright Elia Stupka
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::SeqIO::swiss - Swissprot sequence input/output stream
=head1 SYNOPSIS
It is probably best not to use this object directly, but
rather go through the SeqIO handler system. Go:
$stream = Bio::SeqIO->new(-file => $filename, -format => 'swiss');
while ( my $seq = $stream->next_seq() ) {
# do something with $seq
}
=head1 DESCRIPTION
This object can transform Bio::Seq objects to and from swissprot flat
file databases.
There is a lot of flexibility here about how to dump things which I need
to document fully.
=head2 Optional functions
=over 3
=item _show_dna()
(output only) shows the dna or not
=item _post_sort()
(output only) provides a sorting func which is applied to the FTHelpers
before printing
=item _id_generation_func()
This is function which is called as
print "ID ", $func($seq), "\n";
To generate the ID line. If it is not there, it generates a sensible ID
line using a number of tools.
If you want to output annotations in swissprot format they need to be
stored in a Bio::Annotation::Collection object which is accessible
through the Bio::SeqI interface method L<annotation()|annotation>.
The following are the names of the keys which are polled from a
L<Bio::Annotation::Collection> object.
reference - Should contain Bio::Annotation::Reference objects
comment - Should contain Bio::Annotation::Comment objects
dblink - Should contain Bio::Annotation::DBLink objects
gene_name - Should contain Bio::Annotation::SimpleValue object
=back
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this
and other Bioperl modules. Send your comments and suggestions preferably
to one of the Bioperl mailing lists.
Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bio.perl.org/MailList.html - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution.
Bug reports can be submitted via email or the web:
bioperl-bugs@bio.perl.org
http://bugzilla.bioperl.org/
=head1 AUTHOR - Elia Stupka
Email elia@tll.org.sg
Describe contact details here
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::SeqIO::swiss;
use vars qw(@ISA);
use strict;
use Bio::SeqIO;
use Bio::SeqIO::FTHelper;
use Bio::SeqFeature::Generic;
use Bio::Species;
use Bio::Tools::SeqStats;
use Bio::Seq::SeqFactory;
use Bio::Annotation::Collection;
use Bio::Annotation::Comment;
use Bio::Annotation::Reference;
use Bio::Annotation::DBLink;
use Bio::Annotation::SimpleValue;
use Bio::Annotation::StructuredValue;
@ISA = qw(Bio::SeqIO);
sub _initialize {
my($self,@args) = @_;
$self->SUPER::_initialize(@args);
# hash for functions for decoding keys.
$self->{'_func_ftunit_hash'} = {};
$self->_show_dna(1); # sets this to one by default. People can change it
if( ! defined $self->sequence_factory ) {
$self->sequence_factory(new Bio::Seq::SeqFactory
(-verbose => $self->verbose(),
-type => 'Bio::Seq::RichSeq'));
}
}
=head2 next_seq
Title : next_seq
Usage : $seq = $stream->next_seq()
Function: returns the next sequence in the stream
Returns : Bio::Seq object
Args :
=cut
sub next_seq {
my ($self,@args) = @_;
my ($pseq,$c,$line,$name,$desc,$acc,$seqc,$mol,$div,
$date,$comment,@date_arr);
my $genename = "";
my ($annotation, %params, @features) = ( new Bio::Annotation::Collection);
$line = $self->_readline;
if( !defined $line) {
return undef; # no throws - end of file
}
if( $line =~ /^\s+$/ ) {
while( defined ($line = $self->_readline) ) {
$line =~ /\S/ && last;
}
}
if( !defined $line ) {
return undef; # end of file
}
# fixed to allow _DIVISION to be optional for bug #946
# see bug report for more information
$line =~ /^ID\s+([^\s_]+)(_([^\s_]+))?\s+([^\s;]+);\s+([^\s;]+);/
|| $self->throw("swissprot stream with no ID. Not swissprot in my book");
if( $3 ) {
$name = "$1$2";
$params{'-division'} = $3;
} else {
$name = $1;
$params{'-division'} = 'UNK';
$params{'-primary_id'} = $1;
}
$params{'-alphabet'} = 'protein';
# this is important to have the id for display in e.g. FTHelper, otherwise
# you won't know which entry caused an error
$params{'-display_id'} = $name;
my $buffer = $line;
BEFORE_FEATURE_TABLE :
until( !defined ($buffer) ) {
$_ = $buffer;
# Exit at start of Feature table
last if /^FT/;
# and at the sequence at the latest HL 05/11/2000
last if /^SQ/;
# Description line(s)
if (/^DE\s+(\S.*\S)/) {
$desc .= $desc ? " $1" : $1;
}
#Gene name
elsif(/^GN\s+(.*)/) {
$genename .= " " if $genename;
$genename .= $1;
# has GN terminated yet?
if($genename =~ s/[\. ]+$//) {
my $gn = Bio::Annotation::StructuredValue->new();
foreach my $gene (split(/ AND /, $genename)) {
$gene =~ s/^\(//;
$gene =~ s/\)$//;
$gn->add_value([-1,-1], split(/ OR /, $gene));
}
$annotation->add_Annotation('gene_name',$gn,
"Bio::Annotation::SimpleValue");
}
}
#accession number(s)
elsif( /^AC\s+(.+)/) {
my @accs = split(/[; ]+/, $1); # allow space in addition
$params{'-accession_number'} = shift @accs
unless defined $params{'-accession_number'};
push @{$params{'-secondary_accessions'}}, @accs;
}
#version number
elsif( /^SV\s+(\S+);?/ ) {
my $sv = $1;
$sv =~ s/\;//;
$params{'-seq_version'} = $sv;
}
#date
elsif( /^DT\s+(.*)/ ) {
my $date = $1;
$date =~ s/\;//;
$date =~ s/\s+$//;
push @{$params{'-dates'}}, $date;
}
# Organism name and phylogenetic information
elsif (/^O[SCG]/) {
my $species = $self->_read_swissprot_Species(\$buffer);
$params{'-species'}= $species;
# now we are one line ahead -- so continue without reading the next
# line HL 05/11/2000
next;
}
# References
elsif (/^R/) {
my $refs = $self->_read_swissprot_References(\$buffer);
foreach my $r (@$refs) {
$annotation->add_Annotation('reference',$r);
}
# now we are one line ahead -- so continue without reading the next
# line HL 05/11/2000
next;
}
#Comments
elsif (/^CC\s{3}(.*)/) {
$comment .= $1;
$comment .= "\n";
while (defined ($buffer = $self->_readline)) {
if ($buffer =~ /^CC\s{3}(.*)/) {
$comment .= $1;
$comment .= "\n";
}
else {
last;
}
}
my $commobj = Bio::Annotation::Comment->new();
# note: don't try to process comments here -- they may contain
# structure. LP 07/30/2000
$commobj->text($comment);
$annotation->add_Annotation('comment',$commobj);
$comment = "";
# now we are one line ahead -- so continue without reading the next
# line HL 05/11/2000
next;
}
#DBLinks
elsif (/^DR\s+(\S+)\;\s+(\S+)\;\s+(\S+)[\;\.](.*)$/) {
my $dblinkobj = Bio::Annotation::DBLink->new();
$dblinkobj->database($1);
$dblinkobj->primary_id($2);
$dblinkobj->optional_id($3);
my $comment = $4;
if(length($comment) > 0) {
# edit comment to get rid of leading space and trailing dot
if( $comment =~ /^\s*(\S+)\./ ) {
$dblinkobj->comment($1);
} else {
$dblinkobj->comment($comment);
}
}
$annotation->add_Annotation('dblink',$dblinkobj);
}
#keywords
elsif( /^KW\s+(.*)$/ ) {
my @kw = split(/\s*\;\s*/,$1);
defined $kw[-1] && $kw[-1] =~ s/\.$//;
push @{$params{'-keywords'}}, @kw;
}
# Get next line. Getting here assumes that we indeed need to read the
# line.
$buffer = $self->_readline;
}
$buffer = $_;
FEATURE_TABLE :
# if there is no feature table, or if we've got beyond, exit loop or don't
# even enter HL 05/11/2000
while (defined ($buffer) && ($buffer =~ /^FT/)) {
my $ftunit = $self->_read_FTHelper_swissprot(\$buffer);
# process ftunit
# when parsing of the line fails we get undef returned
if($ftunit) {
push(@features,
$ftunit->_generic_seqfeature($self->location_factory(),
$params{'-seqid'}, "SwissProt"));
} else {
$self->warn("failed to parse feature table line for seq " .
$params{'-display_id'});
}
}
if( $buffer !~ /^SQ/ ) {
while( defined($_ = $self->_readline) ) {
/^SQ/ && last;
}
}
$seqc = "";
while( defined ($_ = $self->_readline) ) {
/^\/\// && last;
$_ = uc($_);
s/[^A-Za-z]//g;
$seqc .= $_;
}
my $seq= $self->sequence_factory->create
(-verbose => $self->verbose,
%params,
-seq => $seqc,
-desc => $desc,
-features => \@features,
-annotation => $annotation,
);
# The annotation doesn't get added by the contructor
$seq->annotation($annotation);
return $seq;
}
=head2 write_seq
Title : write_seq
Usage : $stream->write_seq($seq)
Function: writes the $seq object (must be seq) to the stream
Returns : 1 for success and 0 for error
Args : array of 1 to n Bio::SeqI objects
=cut
sub write_seq {
my ($self,@seqs) = @_;
foreach my $seq ( @seqs ) {
$self->throw("Attempting to write with no seq!") unless defined $seq;
if( ! ref $seq || ! $seq->isa('Bio::SeqI') ) {
$self->warn(" $seq is not a SeqI compliant module. Attempting to dump, but may fail!");
}
my $i;
my $str = $seq->seq;
my $mol;
my $div;
my $len = $seq->length();
if ( !$seq->can('division') || ! defined ($div = $seq->division()) ) {
$div = 'UNK';
}
if( ! $seq->can('alphabet') || ! defined ($mol = $seq->alphabet) ) {
$mol = 'XXX';
}
my $temp_line;
if( $self->_id_generation_func ) {
$temp_line = &{$self->_id_generation_func}($seq);
} else {
#$temp_line = sprintf ("%10s STANDARD; %3s; %d AA.",
# $seq->primary_id()."_".$div,$mol,$len);
# Reconstructing the ID relies heavily upon the input source having
# been in a format that is parsed as this routine expects it -- that is,
# by this module itself. This is bad, I think, and immediately breaks
# if e.g. the Bio::DB::GenPept module is used as input.
# Hence, switch to display_id(); _every_ sequence is supposed to have
# this. HL 2000/09/03
$mol =~ s/protein/PRT/;
$temp_line = sprintf ("%10s STANDARD; %3s; %d AA.",
$seq->display_id(), $mol, $len);
}
$self->_print( "ID $temp_line\n");
# if there, write the accession line
local($^W) = 0; # supressing warnings about uninitialized fields
if( $self->_ac_generation_func ) {
$temp_line = &{$self->_ac_generation_func}($seq);
$self->_print( "AC $temp_line\n");
} else {
if ($seq->can('accession_number') ) {
$self->_print("AC ",$seq->accession_number,";");
if ($seq->can('get_secondary_accessions') ) {
foreach my $sacc ($seq->get_secondary_accessions) {
$self->_print(" ",$sacc,";");
}
$self->_print("\n");
}
else {
$self->_print("\n");
}
}
# otherwise - cannot print <sigh>
}
# Date lines
if( $seq->can('get_dates') ) {
foreach my $dt ( $seq->get_dates() ) {
$self->_write_line_swissprot_regex("DT ","DT ",
$dt,"\\s\+\|\$",80);
}
}
#Definition lines
$self->_write_line_swissprot_regex("DE ","DE ",$seq->desc(),"\\s\+\|\$",80);
#Gene name
if ((my @genes = $seq->annotation->get_Annotations('gene_name') ) ) {
$self->_print("GN ",
join(' OR ',
map {
$_->isa("Bio::Annotation::StructuredValue") ?
$_->value(-joins => [" AND ", " OR "]) :
$_->value();
} @genes),
".\n");
}
# Organism lines
if ($seq->can('species') && (my $spec = $seq->species)) {
my($species, @class) = $spec->classification();
my $genus = $class[0];
my $OS = "$genus $species";
if ($class[$#class] =~ /viruses/i) {
# different OS / OC syntax for viruses LP 09/16/2000
shift @class;
}
if (my $ssp = $spec->sub_species) {
$OS .= " $ssp";
}
foreach (($spec->variant, $spec->common_name)) {
$OS .= " ($_)" if $_;
}
$self->_print( "OS $OS.\n");
my $OC = join('; ', reverse(@class)) .'.';
$self->_write_line_swissprot_regex("OC ","OC ",$OC,"\; \|\$",80);
if ($spec->organelle) {
$self->_write_line_swissprot_regex("OG ","OG ",$spec->organelle,"\; \|\$",80);
}
if ($spec->ncbi_taxid) {
$self->_print("OX NCBI_TaxID=".$spec->ncbi_taxid.";\n");
}
}
# Reference lines
my $t = 1;
foreach my $ref ( $seq->annotation->get_Annotations('reference') ) {
$self->_print( "RN [$t]\n");
# changed by lorenz 08/03/00
# j.gilbert and h.lapp agreed that the rp line in swissprot seems
# more like a comment than a parseable value, so print it as is
if ($ref->rp) {
$self->_write_line_swissprot_regex("RP ","RP ",$ref->rp,
"\\s\+\|\$",80);
}
if ($ref->comment) {
$self->_write_line_swissprot_regex("RC ","RC ",$ref->comment,
"\\s\+\|\$",80);
}
if ($ref->medline) {
# new RX format in swissprot LP 09/17/00
if ($ref->pubmed) {
$self->_write_line_swissprot_regex("RX ","RX ",
"MEDLINE=".$ref->medline.
"; PubMed=".$ref->pubmed.";",
"\\s\+\|\$",80);
} else {
$self->_write_line_swissprot_regex("RX MEDLINE; ","RX MEDLINE; ",
$ref->medline.".","\\s\+\|\$",80);
}
}
my $author = $ref->authors .';' if($ref->authors);
my $title = $ref->title .';' if( $ref->title);
$self->_write_line_swissprot_regex("RA ","RA ",$author,"\\s\+\|\$",80);
$self->_write_line_swissprot_regex("RT ","RT ",$title,"\\s\+\|\$",80);
$self->_write_line_swissprot_regex("RL ","RL ",$ref->location,"\\s\+\|\$",80);
$t++;
}
# Comment lines
foreach my $comment ( $seq->annotation->get_Annotations('comment') ) {
foreach my $cline (split ("\n", $comment->text)) {
while (length $cline > 74) {
$self->_print("CC ",(substr $cline,0,74),"\n");
$cline = substr $cline,74;
}
$self->_print("CC ",$cline,"\n");
}
}
foreach my $dblink ( $seq->annotation->get_Annotations('dblink') )
{
if (defined($dblink->comment)&&($dblink->comment)) {
$self->_print("DR ",$dblink->database,"; ",$dblink->primary_id,"; ",
$dblink->optional_id,"; ",$dblink->comment,".\n");
} elsif($dblink->optional_id) {
$self->_print("DR ",$dblink->database,"; ",
$dblink->primary_id,"; ",
$dblink->optional_id,".\n");
}
else {
$self->_print("DR ",$dblink->database,
"; ",$dblink->primary_id,"; ","-.\n");
}
}
# if there, write the kw line
{
my( $kw );
if( my $func = $self->_kw_generation_func ) {
$kw = &{$func}($seq);
} elsif( $seq->can('keywords') ) {
$kw = $seq->keywords;
if( ref($kw) =~ /ARRAY/i ) {
$kw = join("; ", @$kw);
}
$kw .= '.' if( $kw !~ /\.$/ );
}
$self->_write_line_swissprot_regex("KW ","KW ",
$kw, "\\s\+\|\$",80);
}
#Check if there are seqfeatures before printing the FT line
my @feats = $seq->can('top_SeqFeatures') ? $seq->top_SeqFeatures : ();
if ($feats[0]) {
if( defined $self->_post_sort ) {
# we need to read things into an array. Process. Sort them. Print 'em
my $post_sort_func = $self->_post_sort();
my @fth;
foreach my $sf ( @feats ) {
push(@fth,Bio::SeqIO::FTHelper::from_SeqFeature($sf,$seq));
}
@fth = sort { &$post_sort_func($a,$b) } @fth;
foreach my $fth ( @fth ) {
$self->_print_swissprot_FTHelper($fth);
}
} else {
# not post sorted. And so we can print as we get them.
# lower memory load...
foreach my $sf ( @feats ) {
my @fth = Bio::SeqIO::FTHelper::from_SeqFeature($sf,$seq);
foreach my $fth ( @fth ) {
if( ! $fth->isa('Bio::SeqIO::FTHelper') ) {
$sf->throw("Cannot process FTHelper... $fth");
}
$self->_print_swissprot_FTHelper($fth);
}
}
}
if( $self->_show_dna() == 0 ) {
return;
}
}
# finished printing features.
# molecular weight
my $mw = ${Bio::Tools::SeqStats->get_mol_wt($seq->primary_seq)}[0];
# checksum
# was crc32 checksum, changed it to crc64
my $crc64 = $self->_crc64(\$str);
$self->_print( sprintf("SQ SEQUENCE %4d AA; %d MW; %16s CRC64;\n",
$len,$mw,$crc64));
$self->_print( " ");
my $linepos;
for ($i = 0; $i < length($str); $i += 10) {
$self->_print( substr($str,$i,10), " ");
$linepos += 11;
if( ($i+10)%60 == 0 && (($i+10) < length($str))) {
$self->_print( "\n ");
}
}
$self->_print( "\n//\n");
$self->flush if $self->_flush_on_write && defined $self->_fh;
return 1;
}
}
# Thanks to James Gilbert for the following two. LP 08/01/2000
=head2 _generateCRCTable
Title : _generateCRCTable
Usage :
Function:
Example :
Returns :
Args :
=cut
sub _generateCRCTable {
# 10001000001010010010001110000100
# 32
my $poly = 0xEDB88320;
my ($self) = shift;
$self->{'_crcTable'} = [];
foreach my $i (0..255) {
my $crc = $i;
for (my $j=8; $j > 0; $j--) {
if ($crc & 1) {
$crc = ($crc >> 1) ^ $poly;
}
else {
$crc >>= 1;
}
}
${$self->{'_crcTable'}}[$i] = $crc;
}
}
=head2 _crc32
Title : _crc32
Usage :
Function:
Example :
Returns :
Args :
=cut
sub _crc32 {
my( $self, $str ) = @_;
$self->throw("Argument to crc32() must be ref to scalar")
unless ref($str) eq 'SCALAR';
$self->_generateCRCTable() unless exists $self->{'_crcTable'};
my $len = length($$str);
my $crc = 0xFFFFFFFF;
for (my $i = 0; $i < $len; $i++) {
# Get upper case value of each letter
my $int = ord uc substr $$str, $i, 1;
$crc = (($crc >> 8) & 0x00FFFFFF) ^
${$self->{'_crcTable'}}[ ($crc ^ $int) & 0xFF ];
}
return $crc;
}
=head2 _crc64
Title : _crc64
Usage :
Function:
Example :
Returns :
Args :
=cut
sub _crc64{
my ($self, $sequence) = @_;
my $POLY64REVh = 0xd8000000;
my @CRCTableh = 256;
my @CRCTablel = 256;
my $initialized;
my $seq = $$sequence;
my $crcl = 0;
my $crch = 0;
if (!$initialized) {
$initialized = 1;
for (my $i=0; $i<256; $i++) {
my $partl = $i;
my $parth = 0;
for (my $j=0; $j<8; $j++) {
my $rflag = $partl & 1;
$partl >>= 1;
$partl |= (1 << 31) if $parth & 1;
$parth >>= 1;
$parth ^= $POLY64REVh if $rflag;
}
$CRCTableh[$i] = $parth;
$CRCTablel[$i] = $partl;
}
}
foreach (split '', $seq) {
my $shr = ($crch & 0xFF) << 24;
my $temp1h = $crch >> 8;
my $temp1l = ($crcl >> 8) | $shr;
my $tableindex = ($crcl ^ (unpack "C", $_)) & 0xFF;
$crch = $temp1h ^ $CRCTableh[$tableindex];
$crcl = $temp1l ^ $CRCTablel[$tableindex];
}
my $crc64 = sprintf("%08X%08X", $crch, $crcl);
return $crc64;
}
=head2 _print_swissprot_FTHelper
Title : _print_swissprot_FTHelper
Usage :
Function:
Example :
Returns :
Args :
=cut
sub _print_swissprot_FTHelper {
my ($self,$fth,$always_quote) = @_;
$always_quote ||= 0;
my ($start,$end) = ('?', '?');
if( ! ref $fth || ! $fth->isa('Bio::SeqIO::FTHelper') ) {
$fth->warn("$fth is not a FTHelper class. ".
"Attempting to print, but there could be tears!");
}
if( $fth->loc =~ /(\?|\d+|\>\d+|<\d+)?\.\.(\?|\d+|<\d+|>\d+)?/ ) {
$start = $1 if defined $1;
$end = $2 if defined $2;
# to_FTString only returns one value when start == end, #JB955
# so if no match is found, assume it is both start and end #JB955
} else {
$start = $end = $fth->loc;
}
my $desc = "";
$desc = @{$fth->field->{"description"}}[0]."."
if exists $fth->field->{"description"};
$self->_write_line_swissprot_regex(sprintf("FT %-8s %6s %6s ",
substr($fth->key,0,8),
$start,$end),
"FT ",
$desc.'.','\s+|$',80);
}
#'
=head2 _read_swissprot_References
Title : _read_swissprot_References
Usage :
Function: Reads references from swissprot format. Internal function really
Example :
Returns :
Args :
=cut
sub _read_swissprot_References{
my ($self,$buffer) = @_;
my (@refs);
my ($b1, $b2, $rp, $title, $loc, $au, $med, $com, $pubmed);
if ($$buffer !~ /^RP/) {
$$buffer = $self->_readline;
}
if( !defined $$buffer ) { return undef; }
if( $$buffer =~ /^RP/ ) {
if ($$buffer =~ /^RP (SEQUENCE OF (\d+)-(\d+).*)/) {
$rp=$1;
$b1=$2;
$b2=$3;
}
elsif ($$buffer =~ /^RP (.*)/) {
$rp=$1;
}
}
while( defined ($_ = $self->_readline) ) {
#/^CC/ && last;
/^RN/ && last; # separator between references ! LP 07/25/2000
#/^SQ/ && last; # there may be sequences without CC lines! HL 05/11/2000
/^[^R]/ && last; # may be the safest exit point HL 05/11/2000
/^RX MEDLINE;\s+(\d+)/ && do {$med=$1};
/^RX MEDLINE=(\d+);\s+PubMed=(\d+);/ && do {$med=$1;$pubmed=$2};
/^RA (.*)/ && do { $au .= $au ? " $1" : $1; next;};
/^RT (.*)/ && do { $title .= $title ? " $1" : $1; next;};
/^RL (.*)/ && do { $loc .= $loc ? " $1" : $1; next;};
/^RC (.*)/ && do { $com .= $com ? " $1" : $1; next;};
}
my $ref = new Bio::Annotation::Reference;
$au =~ s/;\s*$//g;
if( defined $title ) {
$title =~ s/;\s*$//g;
}
$ref->start($b1);
$ref->end($b2);
$ref->authors($au);
$ref->title($title);
$ref->location($loc);
$ref->medline($med);
$ref->pubmed($pubmed) if (defined $pubmed);
$ref->comment($com);
$ref->rp($rp);
push(@refs,$ref);
$$buffer = $_;
return \@refs;
}
=head2 _read_swissprot_Species
Title : _read_swissprot_Species
Usage :
Function: Reads the swissprot Organism species and classification
lines.
Example :
Returns : A Bio::Species object
Args :
=cut
sub _read_swissprot_Species {
my( $self, $buffer ) = @_;
my $org;
$_ = $$buffer;
my( $subspecies, $species, $genus, $common, $variant, $ncbi_taxid );
my @class;
my ($binomial, $descr);
my $osline = "";
while (defined( $_ ||= $self->_readline )) {
last unless /^O[SCGX]/;
# believe it or not, but OS may come multiple times -- at this time
# we can't capture multiple species
if(/^OS\s+(\S.+)/ && (! defined($binomial))) {
$osline .= " " if $osline;
$osline .= $1;
if($osline =~ s/(,|, and|\.)$//) {
($binomial, $descr) = $osline =~ /(\S[^\(]+)(.*)/;
($genus, $species, $subspecies) = split(/\s+/, $binomial);
$species = "sp." unless $species;
while($descr =~ /\(([^\)]+)\)/g) {
my $item = $1;
# strain etc may not necessarily come first (yes, swissprot
# is messy)
if((! defined($variant)) &&
(($item =~ /(^|[^\(\w])([Ss]train|isolate|serogroup|serotype|subtype|clone)\b/) ||
($item =~ /^(biovar|pv\.|type\s+)/))) {
$variant = $item;
} elsif($item =~ s/^subsp\.\s+//) {
if(! $subspecies) {
$subspecies = $item;
} elsif(! $variant) {
$variant = $item;
}
} elsif(! defined($common)) {
# we're only interested in the first common name
$common = $item;
if((index($common, '(') >= 0) &&
(index($common, ')') < 0)) {
$common .= ')';
}
}
}
}
}
elsif (s/^OC\s+//) {
push(@class, split /[\;\.]\s*/);
if($class[0] =~ /viruses/i) {
# viruses have different OS/OC syntax
my @virusnames = split(/\s+/, $binomial);
$species = (@virusnames > 1) ? pop(@virusnames) : '';
$genus = join(" ", @virusnames);
$subspecies = undef;
}
}
elsif (/^OG\s+(.*)/) {
$org = $1;
}
elsif (/^OX\s+(.*)/ && (! defined($ncbi_taxid))) {
my $taxstring = $1;
# we only keep the first one and ignore all others
if ($taxstring =~ /NCBI_TaxID=([\w\d]+)/) {
$ncbi_taxid = $1;
} else {
$self->throw("$taxstring doesn't look like NCBI_TaxID");
}
}
$_ = undef; # Empty $_ to trigger read of next line
}
$$buffer = $_;
# Don't make a species object if it is "Unknown" or "None"
return if $genus =~ /^(Unknown|None)$/i;
if ($class[$#class] eq $genus) {
push( @class, $species );
} else {
push( @class, $genus, $species );
}
@class = reverse @class;
my $taxon = Bio::Species->new();
$taxon->classification( \@class, "FORCE" ); # no name validation please
$taxon->common_name( $common ) if $common;
$taxon->sub_species( $subspecies ) if $subspecies;
$taxon->organelle ( $org ) if $org;
$taxon->ncbi_taxid ( $ncbi_taxid ) if $ncbi_taxid;
$taxon->variant($variant) if $variant;
# done
return $taxon;
}
=head2 _filehandle
Title : _filehandle
Usage : $obj->_filehandle($newval)
Function:
Example :
Returns : value of _filehandle
Args : newvalue (optional)
=cut
# inherited from SeqIO.pm ! HL 05/11/2000
=head2 _read_FTHelper_swissprot
Title : _read_FTHelper_swissprot
Usage : _read_FTHelper_swissprot(\$buffer)
Function: reads the next FT key line
Example :
Returns : Bio::SeqIO::FTHelper object
Args : filehandle and reference to a scalar
=cut
sub _read_FTHelper_swissprot {
# initial version implemented by HL 05/10/2000
# FIXME this may not be perfect, so please review
my ($self,$buffer) = @_;
my ($key, # The key of the feature
$loc, # The location line from the feature
$desc, # The descriptive text
);
if ($$buffer =~ /^FT (\w+)\s+([\d\?\<]+)\s+([\d\?\>]+)\s*(.*)$/) {
$key = $1;
my $loc1 = $2;
my $loc2 = $3;
$loc = "$loc1..$loc2";
if($4 && (length($4) > 0)) {
$desc = $4;
chomp($desc);
} else {
$desc = "";
}
# Read all the continuation lines up to the next feature
while (defined($_ = $self->_readline) && /^FT\s{20,}(\S.*)$/) {
$desc .= $1;
chomp($desc);
}
$desc =~ s/\.$//;
} else {
# No feature key. What's this?
$self->warn("No feature key in putative feature table line: $_");
return;
}
# Put the first line of the next feature into the buffer
$$buffer = $_;
# Make the new FTHelper object
my $out = new Bio::SeqIO::FTHelper(-verbose => $self->verbose());
$out->key($key);
$out->loc($loc);
# store the description if there is one
if($desc && (length($desc) > 0)) {
$out->field->{"description"} ||= [];
push(@{$out->field->{"description"}}, $desc);
}
return $out;
}
=head2 _write_line_swissprot
Title : _write_line_swissprot
Usage :
Function: internal function
Example :
Returns :
Args :
=cut
sub _write_line_swissprot{
my ($self,$pre1,$pre2,$line,$length) = @_;
$length || die "Miscalled write_line_swissprot without length. Programming error!";
my $subl = $length - length $pre2;
my $linel = length $line;
my $i;
my $sub = substr($line,0,$length - length $pre1);
$self->_print( "$pre1$sub\n");
for($i= ($length - length $pre1);$i < $linel;) {
$sub = substr($line,$i,($subl));
$self->_print( "$pre2$sub\n");
$i += $subl;
}
}
=head2 _write_line_swissprot_regex
Title : _write_line_swissprot_regex
Usage :
Function: internal function for writing lines of specified
length, with different first and the next line
left hand headers and split at specific points in the
text
Example :
Returns : nothing
Args : file handle, first header, second header, text-line, regex for line breaks, total line length
=cut
sub _write_line_swissprot_regex {
my ($self,$pre1,$pre2,$line,$regex,$length) = @_;
#print STDOUT "Going to print with $line!\n";
$length || die "Miscalled write_line_swissprot without length. Programming error!";
if( length $pre1 != length $pre2 ) {
print STDERR "len 1 is ", length $pre1, " len 2 is ", length $pre2, "\n";
die "Programming error - cannot called write_line_swissprot_regex with different length \npre1 ($pre1) and \npre2 ($pre2) tags!";
}
my $subl = $length - (length $pre1) -1 ;
my @lines;
while($line =~ m/(.{1,$subl})($regex)/g) {
push(@lines, $1.$2);
}
my $s = shift @lines;
$self->_print( "$pre1$s\n");
foreach my $s ( @lines ) {
$self->_print( "$pre2$s\n");
}
}
=head2 _post_sort
Title : _post_sort
Usage : $obj->_post_sort($newval)
Function:
Returns : value of _post_sort
Args : newvalue (optional)
=cut
sub _post_sort{
my $obj = shift;
if( @_ ) {
my $value = shift;
$obj->{'_post_sort'} = $value;
}
return $obj->{'_post_sort'};
}
=head2 _show_dna
Title : _show_dna
Usage : $obj->_show_dna($newval)
Function:
Returns : value of _show_dna
Args : newvalue (optional)
=cut
sub _show_dna{
my $obj = shift;
if( @_ ) {
my $value = shift;
$obj->{'_show_dna'} = $value;
}
return $obj->{'_show_dna'};
}
=head2 _id_generation_func
Title : _id_generation_func
Usage : $obj->_id_generation_func($newval)
Function:
Returns : value of _id_generation_func
Args : newvalue (optional)
=cut
sub _id_generation_func{
my $obj = shift;
if( @_ ) {
my $value = shift;
$obj->{'_id_generation_func'} = $value;
}
return $obj->{'_id_generation_func'};
}
=head2 _ac_generation_func
Title : _ac_generation_func
Usage : $obj->_ac_generation_func($newval)
Function:
Returns : value of _ac_generation_func
Args : newvalue (optional)
=cut
sub _ac_generation_func{
my $obj = shift;
if( @_ ) {
my $value = shift;
$obj->{'_ac_generation_func'} = $value;
}
return $obj->{'_ac_generation_func'};
}
=head2 _sv_generation_func
Title : _sv_generation_func
Usage : $obj->_sv_generation_func($newval)
Function:
Returns : value of _sv_generation_func
Args : newvalue (optional)
=cut
sub _sv_generation_func{
my $obj = shift;
if( @_ ) {
my $value = shift;
$obj->{'_sv_generation_func'} = $value;
}
return $obj->{'_sv_generation_func'};
}
=head2 _kw_generation_func
Title : _kw_generation_func
Usage : $obj->_kw_generation_func($newval)
Function:
Returns : value of _kw_generation_func
Args : newvalue (optional)
=cut
sub _kw_generation_func{
my $obj = shift;
if( @_ ) {
my $value = shift;
$obj->{'_kw_generation_func'} = $value;
}
return $obj->{'_kw_generation_func'};
}
1;
]]>