Bio::Tools Coil
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Summary
Bio::Tools::Coil
Package variables
No package variables defined.
Included modules
Bio::Root::IO
Bio::Root::Root
Bio::SeqFeature::FeaturePair
Bio::SeqFeature::Generic
Inherit
Bio::Root::IO Bio::Root::Root
Synopsis
 use Bio::Tools::Coil
my $parser = new Bio::Tools::Coil();
while( my $sp_feat = $parser->next_result($file) ) {
#do something
#eg
push @sp_feat, $sp_feat;
}
Description
 Parser for Coil output
Methods
_add_predictionDescriptionCode
_predictions_parsedDescriptionCode
_read_fastaDescriptionCode
_resultDescriptionCode
create_featureDescriptionCode
new
No description
Code
next_resultDescriptionCode
parse_resultsDescriptionCode
Methods description
_add_predictioncode    nextTop
 Title   : _add_prediction()
Usage : $obj->_add_prediction($feat)
Function: internal
Example :
Returns :
_predictions_parsedcodeprevnextTop
 Title   : _predictions_parsed
Usage : $obj->_predictions_parsed
Function: internal
Example :
Returns : TRUE or FALSE
_read_fastacodeprevnextTop
 Title   : _read_fasta
Usage : obj->_read_fasta($file)
Function: Internal(not to be used directly)
Returns :
Args :
_resultcodeprevnextTop
 Title   : _result
Usage : $feat = $obj->_result()
Function: internal
Example :
Returns :
create_featurecodeprevnextTop
 Title   : create_feature
Usage : obj->create_feature(\%feature)
Function: Internal(not to be used directly)
Returns :
Args :
next_resultcodeprevnextTop
 Title   : next_result
Usage : while($feat = $coil->next_result($file)) {
# do something
}
Function: Returns the next protein feature of the coil output file
Returns :
Args :
parse_resultscodeprevnextTop
 Title   : parse_results
Usage : obj->parse_results
Function: Parses the coil output. Automatically called by
next_result() if not yet done.
Example :
Returns :
Methods code
_add_predictiondescriptionprevnextTop
sub _add_prediction {
    my ($self, $gene) = @_;

    if(! exists($self->{'_feats'})) {
        $self->{'_feats'} = [];
    }
    push(@{$self->{'_feats'}}, $gene);
}
_predictions_parseddescriptionprevnextTop
sub _predictions_parsed {
    my ($self, $val) = @_;

    $self->{'_preds_parsed'} = $val if $val;
    if(! exists($self->{'_preds_parsed'})) {
        $self->{'_preds_parsed'} = 0;
    }
    return $self->{'_preds_parsed'};
}
_read_fastadescriptionprevnextTop
sub _read_fasta {
        local (*FILE) = @_;
        my( $id , $seq , %name2seq);#bala
while (<FILE>) { chomp;#bala
if (/^>(\S+)/) { my $new_id = $1; if ($id) { $name2seq{$id} = $seq; } $id = $new_id ; $seq = "" ; } elsif (eof) { if ($id) { $seq .= $_ ;#bala line instead of $_
$name2seq{$id} = $seq; } } else { $seq .= $_ } } return %name2seq; } 1;
}
_resultdescriptionprevnextTop
sub _result {
    my ($self) = @_;

    return undef unless(exists($self->{'_feats'}) && @{$self->{'_feats'}});
    return shift(@{$self->{'_feats'}});
}
create_featuredescriptionprevnextTop
sub create_feature {
       my ($self, $feat) = @_;


       # create feature object
my $feature = Bio::SeqFeature::Generic->new(-seq_id => $feat->{name}, -start => $feat->{start}, -end => $feat->{end}, -score => $feat->{score}, -source => $feat->{source}, -primary => $feat->{primary}, -logic_name => $feat->{logic_name}, ); $feature->add_tag_value('evalue',0); $feature->add_tag_value('percent_id','NULL'); $feature->add_tag_value("hid",$feat->{primary}); return $feature;
}
newdescriptionprevnextTop
sub new {
      my($class,@args) = @_;

      my $self = $class->SUPER::new(@args);
      $self->_initialize_io(@args);

      return $self;
}
next_resultdescriptionprevnextTop
sub next_result {
    
    my ($self,$resfile) = @_;
    my $gene;

    $self->parse_results($resfile) unless $self->_predictions_parsed();

    $gene = $self->_result();

    return $gene;
}
parse_resultsdescriptionprevnextTop
sub parse_results {
  my ($self,$resfile) = @_;
        my $filehandle = $resfile;
        my %result_hash =_read_fasta($filehandle);#bala no file handle
my @ids = keys %result_hash; my @feats; foreach my $id (keys %result_hash){ my $pep = reverse ($result_hash{$id}); my $count = my $switch = 0; my ($start, $end); while (my $aa = chop $pep) { $count++; if (!$switch && $aa eq "x") { $start = $count; $switch = 1; } elsif ($switch && $aa ne "x") { $end = $count-1; my (%feature); $feature{name} = $id; $feature{start} = $start; $feature{end} = $end; $feature{source} = "Coils"; $feature{primary} = 'ncoils'; ($feature{program}) = 'ncoils'; $feature{logic_name} = 'Coils'; my $new_feat = $self->create_feature (\%feature); $self->_add_prediction($new_feat); $switch = 0; } } } $self->_predictions_parsed(1);
}
General documentation
FEEDBACKTop
Mailing ListsTop
 User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion http://bioperl.org/MailList.shtml - About the mailing lists
Reporting BugsTop
 Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
email or the web:
bioperl-bugs@bio.perl.org http://bugzilla.bioperl.org/
AUTHORTop
 Based on the EnsEMBL module Bio::EnsEMBL::Pipeline::Runnable::Protein::Coil
originally written by Marc Sohrmann (ms2@sanger.ac.uk)
Written in BioPipe by Balamurugan Kumarasamy <savikalpa@fugu-sg.org>
Cared for by the Fugu Informatics team (fuguteam@fugu-sg.org)
APPENDIXTop
 The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _