Raw content of Bio::Tools::EPCR
<![CDATA[# $Id: EPCR.pm,v 1.8 2002/12/01 00:05:21 jason Exp $
#
# BioPerl module for Bio::Tools::EPCR
#
# Cared for by Jason Stajich <jason@bioperl.org>
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::EPCR - Parse ePCR output and make features
=head1 SYNOPSIS
# A simple annotation pipeline wrapper for ePCR data
# assuming ePCR data is already generated in file seq1.epcr
# and sequence data is in fasta format in file called seq1.fa
use Bio::Tools::EPCR;
use Bio::SeqIO;
my $parser = new Bio::Tools::EPCR(-file => 'seq1.epcr');
my $seqio = new Bio::SeqIO(-format => 'fasta', -file => 'seq1.fa');
my $seq = $seqio->next_seq || die("cannot get a seq object from SeqIO");
while( my $feat = $parser->next_feature ) {
# add EPCR annotation to a sequence
$seq->add_SeqFeature($feat);
}
my $seqout = new Bio::SeqIO(-format => 'embl');
$seqout->write_seq($seq);
=head1 DESCRIPTION
This object serves as a parser for ePCR data, creating a
Bio::SeqFeatureI for each ePCR hit. These can be processed or added
as annotation to an existing Bio::SeqI object for the purposes of
automated annotation.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/MailList.shtml - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
email or the web:
bioperl-bugs@bioperl.org
http://bugzilla.bioperl.org/
=head1 AUTHOR - Jason Stajich
Email jason@bioperl.org
Describe contact details here
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::Tools::EPCR;
use vars qw(@ISA);
use strict;
use Bio::Root::Root;
use Bio::SeqAnalysisParserI;
use Bio::SeqFeature::FeaturePair;
use Bio::SeqFeature::Generic;
@ISA = qw(Bio::Root::Root Bio::SeqAnalysisParserI Bio::Root::IO );
=head2 new
Title : new
Usage : my $epcr = new Bio::Tools::EPCR(-file => $file);
Function: Initializes a new EPCR parser
Returns : Bio::Tools::EPCR
Args : -fh => filehandle
OR
-file => filename
=cut
sub new {
my($class,@args) = @_;
my $self = $class->SUPER::new(@args);
$self->_initialize_io(@args);
return $self;
}
=head2 next_feature
Title : next_feature
Usage : $seqfeature = $obj->next_feature();
Function: Returns the next feature available in the analysis result, or
undef if there are no more features.
Example :
Returns : A Bio::SeqFeatureI implementing object, or undef if there are no
more features.
Args : none
=cut
sub next_feature {
my ($self) = @_;
my $line = $self->_readline;
return undef unless defined($line);
chomp($line);
my($seqname,$location,$mkrname, $rest) = split(/\s+/,$line,4);
my ($start,$end) = ($location =~ /(\S+)\.\.(\S+)/);
# If we require that e-PCR is run with D=1 we can detect a strand
# for now hardcoded to 0
my $strand = 0;
my $markerfeature = new Bio::SeqFeature::Generic ( '-start' => $start,
'-end' => $end,
'-strand' => $strand,
'-source' => 'e-PCR',
'-primary' => 'sts',
'-seq_id' => $seqname,
'-tag' => {
'name'=> $mkrname,
'note'=> $rest,
});
return $markerfeature;
}
1;
]]>