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Summary

Bio::Variation::AAReverseMutate - point mutation and codon
information from single amino acid changes

Package variables

Included modules

Inherit

Bio::Root::Root

Synopsis

    $aamut = new Bio::Variation::AAReverseMutate
                 (-aa_ori => 'F', 
		  -aa_mut => 'S',
		  -codon_ori => 'ttc', # optional
		  -codon_table => '3'  # defaults to 1
		  );

   @points = $aamut->each_Variant;

   if (scalar @points > 0 ) {
       foreach $rnachange ( @points ) {
   	   # $rnachange is a Bio::Variation::RNAChange object
   	   print " ", $rnachange->allele_ori->seq, ">", 
	   $rnachange->allele_mut->seq, " in ",
   	   $rnachange->codon_ori, ">", $rnachange->codon_mut,
   	   " at position ", $rnachange->codon_pos, "\n";
       }
   } else  {
       print "No point mutations possible\n",
   }

Description

Bio::Variation::AAReverseMutate objects take in reference and mutated
amino acid information and deduces potential point mutations at RNA
level leading to this change. The choice can be further limited by
letting the object know what is the the codon in the reference
sequence. The results are returned as Bio::Variation::RNAChange
objects.

Methods

Methods description

 Title   : aa_mut
 Usage   : $obj->aa_mut();
 Function: 

            Sets and returns the mutated allele sequence.  If value is not
            set, returns false. 

 Example : 
 Returns : string
 Args    : single character amino acid code

 Title   : aa_ori
 Usage   : $obj->aa_ori();
 Function: 

            Sets and returns original aa sequence.  If value is not
            set, returns false. 

            Amino acid sequences are stored in upper case characters,
            others in lower case.

 Example : 
 Returns : string
 Args    : single character amino acid code

 Title   : codon_ori
 Usage   : $obj->codon_ori();
 Function: 

            Sets and returns codon_ori triplet.  If value is not set,
            returns false.  The string has to be three characters
            long. The chracter content is not checked.

 Example : 
 Returns : string
 Args    : string

 Title   : codon_table
 Usage   : $obj->codon_table();
 Function: 

            Sets and returns the codon table id of the RNA
            If value is not set, returns 1, 'universal' code, as the default.

 Example : 
 Returns : integer
 Args    : none if get, the new value if set

 Title   : each_Variant
 Usage   : $obj->each_Variant();
 Function: 

            Returns a list of Variants.

 Example : 
 Returns : list of Variants
 Args    : none

Methods code

sub aa_mut {

    my ($self,$value) = @_;
    if( defined $value) {
	if (  uc($value) !~ /^[ARNDCQEGHILKMFPSTWYVBZX*]$/ ) {
	    $self->throw("'$value' is not a valid one letter amino acid symbol\n");
	} else {
	    $self->{'aa_mut'} = uc $value;
	}
    }
    return $self->{'aa_mut'};

}

sub aa_ori {

    my ($self,$value) = @_;
    if( defined $value) {
	if (  uc($value) !~ /^[ARNDCQEGHILKMFPSTWYVBZX*]$/ ) {
	    $self->throw("'$value' is not a valid one letter amino acid symbol\n");
	} else {
	    $self->{'aa_ori'} = uc $value;
	}
    }
    return $self->{'aa_ori'};

}

sub codon_ori {

    my ($self,$value) = @_;
    if( defined $value) {
	if (length $value != 3 or lc $value =~ /[^atgc]/) {
	    $self->warn("Codon string\" $value\" is not valid unique codon");
	}
	$self->{'codon_ori'} = lc $value;
    }
    return $self->{'codon_ori'};

}

sub codon_table {

    my ($self,$value) = @_;
    if( defined $value) {
	if (  not $value =~ /^\d+$/ ) {
	    $self->throw("'$value' is not a valid codon table ID\n".
			"Has to be a positive integer. Defaulting to 1\n");
	} else {
	    $self->{'codon_table'} = $value;
	}
    }
    if( ! exists $self->{'codon_table'} ) {
	return 1;
    } else {
	return $self->{'codon_table'};
    }

}

sub each_Variant {

   my ($self,@args) = @_;
   
   $self->throw("aa_ori is not defined\n") if not defined $self->aa_ori;
   $self->throw("aa_mut is not defined\n") if not defined $self->aa_mut;

   my (@points, $codon_pos, $allele_ori, $allele_mut);
   my $ct  = Bio::Tools::CodonTable->new( '-id' => $self->codon_table );
   foreach my $codon_ori ($ct->revtranslate($self->aa_ori)) {
       next if $self->codon_ori and $self->codon_ori ne $codon_ori;
       foreach my $codon_mut ($ct->revtranslate($self->aa_mut)) {
	   my $k = 0;
	   my $length = 0; 
	   $codon_pos = $allele_ori = $allele_mut = undef;
	   while ($k<3) {
	       my $nt_ori = substr ($codon_ori, $k, 1);
	       my $nt_mut = substr ($codon_mut, $k, 1);
	       if ($nt_ori ne $nt_mut) {
		   $length++;
		   $codon_pos = $k+1;
		   $allele_ori = $nt_ori;
		   $allele_mut = $nt_mut;
	       }
	       $k++;
	   }
	   if ($length == 1) {
	       my $rna = Bio::Variation::RNAChange->new 
		   ('-length'        => '1',     
		    '-codon_ori'     => $codon_ori,
		    '-codon_mut'     => $codon_mut,
		    '-codon_pos'     => $codon_pos,
		    '-isMutation'    => 1
		    );
	       my $all_ori = Bio::Variation::Allele->new('-seq'=>$allele_ori);
	       $rna->allele_ori($all_ori);
	       my $all_mut = Bio::Variation::Allele->new('-seq'=>$allele_mut);
	       $rna->allele_mut($all_mut);
	       push @points, $rna;
	   }
       }
   }
   return @points;
}

1;

}

sub new {

    my($class,@args) = @_;
    my $self = $class->SUPER::new(@args);

    my ($aa_ori, $aa_mut, $codon_ori, $codon_table) =
	$self->_rearrange([qw(AA_ORI
			      AA_MUT
			      CODON
			      CODON_TABLE
			      )],@args);

  $aa_ori && $self->aa_ori($aa_ori);
  $aa_mut  && $self->aa_mut($aa_mut);
  $codon_ori  && $self->codon_ori($codon_ori);
  $codon_table && $self->codon_table($codon_table);

  return $self; # success - we hope!

}

General documentation

User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to the
Bioperl mailing lists Your participation is much appreciated.

  bioperl-l@bioperl.org                         - General discussion
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report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via
email or the web:

  bioperl-bugs@bio.perl.org
  http://bugzilla.bioperl.org/

Email: heikki@ebi.ac.uk
Address:

     EMBL Outstation, European Bioinformatics Institute
     Wellcome Trust Genome Campus, Hinxton
     Cambs. CB10 1SD, United Kingdom

The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _