Bio::EnsEMBL::Analysis::Runnable::ProteinAnnotation Pfam_wormbase
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Bio::EnsEMBL::Analysis::Runnable::ProteinAnnotation
Bio::EnsEMBL::Utils::Exception qw ( throw warning )
Env qw ( PATH )
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Bio::EnsEMBL::Analysis::Runnable::ProteinAnnotation
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Methods
parse_resultsDescriptionCode
run_analysis
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Methods description
parse_resultscode    nextTop
 Title    :  parse_results
Usage : $self->parse_results ($filename)
Function : parses program output to give a set of features
Example :
Returns :
Args : filename (optional, can be filename, filehandle or pipe, not implemented)
Throws :
Methods code
parse_resultsdescriptionprevnextTop
sub parse_results {
    my ($self) = @_;

    my $filehandle;
    my $resfile = $self->resultsfile();
    my @fps;
		

    if (-e $resfile) {
      if (-z $resfile) {  
	print STDERR "Pfam didn't find any hits\n";
	return; 
      } else {
	open (CPGOUT, "<$resfile") or $self->throw("Error opening ", $resfile, "\n "); # 
} } # Example output for sequence ID 2345:
#2345 279 319 PF00400.23 1 38 13.7 0.008 WD40
#2345 323 361 PF00400.23 1 38 22.0 0.0023 WD40
# now
# 12311 49 288 PF00149.20 1 124 ls 90.9 4.4e-24 Metallophos
# 12311 294 517 PF04152.6 1 240 ls 310.9 2.6e-90 Mre11_DNA_bind
# 11845 1 167 PF06653.3 1 160 ls -12.3 0.00016 DUF1164
my $id; while (<CPGOUT>) { chomp; print "$_\n"; if (my ($id, $start, $end, $hid, $hstart, $hend, $skipit,$score, $evalue) = /^(\S+)\s+(\d+)\s+(\d+)\s+(\S+)\s+(\d+)\s+(\d+)\s+(ls)*\s+([\d\.\-\+]+)\s+(\S+)\s+(\S+)/) { print "matched\n"; my $percentIdentity = 0; my $fp= $self->create_protein_feature($start, $end, $score, $id, $hstart, $hend, $hid, $self->analysis, $evalue, $percentIdentity); push @fps, $fp; } } close (CPGOUT); $self->output(\@fps); } 1;
}
run_analysisdescriptionprevnextTop
sub run_analysis {
    my ($self) = @_;

    # run program
print STDERR "running ".$self->program." against ".$self->database."\n"; print STDERR "FILENAME: ".$self->queryfile."\n"; my $cmd = $self->program .' '. $self->analysis->parameters .' '. '-o ' . $self->resultsfile .' '. $self->queryfile; print STDERR "$cmd\n"; $PATH = '/software/worm/bin/hmmer/:/software/worm/iprscan/bin/binaries/:'.$PATH; #for hmmpfam
$PATH = '/software/worm/iprscan/bin/binaries/blast/:'.$PATH; #for blastall
$self->throw ("Error running Pfam_wormbase ".$self->program." on ".$self->queryfile) unless ((system ($cmd)) == 0);
}
General documentation
run_programTop
 Title    : run_program
Usage : $self->program
Function : makes the system call to program
Example :
Returns :
Args :
Throws :