Raw content of Bio::EnsEMBL::Analysis::RunnableDB::Pmatch
<![CDATA[=pod
=head1 NAME
Bio::EnsEMBL::Analysis::RunnableDB::Pmatch - aligns protein sequence to genomic sequence
=head1 DESCRIPTION
Pmatch is a fast alignment program written by Richard Durbin we used
to align species specific proteins to the genome, (We also use it to
align very closely related species proteins sets e.g Mouse to Rat or
Fugu to Tetraodon).
The pmatch source code is available from the
sanger cvs respository in module rd-utils,
(http://cvs.sanger.ac.uk/cgi-bin/viewvc.cgi/rd-utils/?root=ensembl).
The code to run this process in the ensembl code
base can be found in 2 RunnableDBs and a config
file. Bio::EnsEMBL::Analysis::RunnableDB::Pmatch,
Bio::EnsEMBL::Analysis::RunnableDB::BestPmatch and
Bio::EnsEMBL::Analysis::Config::GeneBuild::Pmatch
=head1 CONTACT
Email ensembl-dev@ebi.ac.uk for questions.
=head1 APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
=cut
package Bio::EnsEMBL::Analysis::RunnableDB::Pmatch;
use vars qw(@ISA);
use strict;
use Bio::EnsEMBL::Analysis::RunnableDB::BaseGeneBuild;
use Bio::EnsEMBL::Analysis::Config::GeneBuild::Pmatch qw(PMATCH_BY_LOGIC);
use Bio::EnsEMBL::Analysis::Runnable::Pmatch;
use Bio::EnsEMBL::Utils::Exception qw(throw warning);
use Bio::EnsEMBL::Utils::Argument qw (rearrange);
@ISA = qw (
Bio::EnsEMBL::Analysis::RunnableDB::BaseGeneBuild
);
sub new {
my ($class,@args) = @_;
my $self = $class->SUPER::new(@args);
$self->read_and_check_config($PMATCH_BY_LOGIC);
return $self;
}
sub fetch_input{
my ($self) = @_;
my $slice = $self->fetch_sequence($self->input_id, $self->db,
$self->REPEAT_MASKING);
$self->query($slice);
my %parameters = %{$self->parameters_hash};
my $program = $self->analysis->program_file;
$program = $self->BINARY_LOCATION if(!$program);
my $runnable = Bio::EnsEMBL::Analysis::Runnable::Pmatch
->new(
-query => $self->query,
-program => $program,
-analysis => $self->analysis,
-protein_file => $self->PROTEIN_FILE,
-max_intron_length => $self->MAX_INTRON_LENGTH,
-min_coverage => $self->MIN_COVERAGE,
);
$self->runnable($runnable);
}
sub PROTEIN_FILE{
my ($self, $arg) = @_;
if($arg){
$self->{'PROTEIN_FILE'} = $arg;
}
return $self->{'PROTEIN_FILE'};
}
sub MIN_COVERAGE{
my ($self, $arg) = @_;
if($arg){
$self->{'MIN_COVERAGE'} = $arg;
}
return $self->{'MIN_COVERAGE'};
}
sub BINARY_LOCATION{
my ($self, $arg) = @_;
if($arg){
$self->{'BINARY_LOCATION'} = $arg;
}
return $self->{'BINARY_LOCATION'};
}
sub MAX_INTRON_LENGTH{
my ($self, $arg) = @_;
if($arg){
$self->{'MAX_INTRON_SIZE'} = $arg;
}
return $self->{'MAX_INTRON_SIZE'};
}
sub OUTPUT_DB{
my ($self, $arg) = @_;
if($arg){
$self->{'OUTPUT_DB'} = $arg;
}
return $self->{'OUTPUT_DB'};
}
sub REPEAT_MASKING{
my ($self, $arg) = @_;
if($arg){
throw("Runnable::Pmatch ".$arg." must be an array ref of logic names not .".$arg)
unless(ref($arg) eq 'ARRAY');
$self->{'REPEAT_MASKING'} = $arg;
}
return $self->{'REPEAT_MASKING'};
}
sub read_and_check_config {
my $self = shift;
$self->SUPER::read_and_check_config($PMATCH_BY_LOGIC);
#######
#CHECKS
#######
foreach my $config_var (qw(PROTEIN_FILE
OUTPUT_DB)){
throw("You must define $config_var in config for logic '".
$self->analysis->logic_name."'")
if not defined $self->$config_var;
}
};
sub get_adaptor{
my ($self) = @_;
my $output_db = $self->get_dbadaptor($self->OUTPUT_DB);
return $output_db->get_ProteinAlignFeatureAdaptor;
}
sub write_output{
my ($self) = @_;
my $adaptor = $self->get_adaptor;
my %unique;
FEATURE:foreach my $feature(@{$self->output}){
$feature->analysis($self->analysis);
$feature->slice($self->query) if(!$feature->slice);
my $unique_string = $feature->start." ".$feature->end." ".$feature->score." ".$feature->hseqname;
next FEATURE if($unique{$unique_string});
$unique{$unique_string} = 1;
$self->feature_factory->validate($feature);
eval{
$adaptor->store($feature);
};
if($@){
throw("RunnableDB:store failed, failed to write ".$feature." to ".
"the database ".$adaptor->dbc->dbname." $@");
}
}
return 1;
}
1;
]]>