SeqStoreConverter::vega DanioRerio
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Included modules
SeqStoreConverter::DanioRerio
SeqStoreConverter::vega::VBasicConverter
Inherit
SeqStoreConverter::DanioRerio SeqStoreConverter::vega::VBasicConverter
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Methods
copy_internal_clone_names
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Code
update_clone_info
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Methods code
copy_internal_clone_namesdescriptionprevnextTop
sub copy_internal_clone_names {
    my $self = shift;

    my $target = $self->target();
    my $source = $self->source();
    my $dbh    = $self->dbh();
    $self->debug("Vega danio specific - copying internal clone names to seq_region_attrib");

#get id for 'fpc_clone_id' attribute
$dbh->do("INSERT INTO $target.attrib_type (code,name,description)". "values ('fpc_clone_id','fpc clone','clone id used for linking to Zebrafish webFPC')"); my ($attrib_id) = $dbh->selectrow_array("Select attrib_type_id from $target.attrib_type where code = 'fpc_clone_id'"); warn "No attrib id found\n" unless defined($attrib_id); #get clone details
my $select1_sth = $dbh->prepare ("SELECT seq_region_id, name from $target.seq_region where coord_system_id = 3;"); $select1_sth->execute(); my ($seq_region_id, $embl_name); $select1_sth->bind_columns(\$seq_region_id,\$ embl_name); my $clone_name; my $select2_sth = $dbh->prepare("select name from $source.clone where embl_acc= ?"); my $insert_sth = $dbh->prepare("insert into $target.seq_region_attrib values (?,$attrib_id,?)"); while ($select1_sth->fetch()) { $embl_name =~ s/([\d\w]+).*/$1/; $select2_sth->bind_param(1,$embl_name); $select2_sth->execute; $insert_sth->bind_param(1,$seq_region_id); while (my ($clone_name) = $select2_sth->fetchrow_array()) { $insert_sth->bind_param(2,$clone_name); $insert_sth->execute(); } }
}
update_clone_infodescriptionprevnextTop
sub update_clone_info {
    my $self = shift;
    my $target_cs_name = shift;

    my $target = $self->target();
    my $source = $self->source();
    my $dbh    = $self->dbh();

    # clone_info, current_clone_info
$self->debug("Vega Danio_specific - Transforming clone_id into seq_region_id for clone_info and current_clone_info"); foreach my $table_name ('clone_info','current_clone_info') { my $select_st1 = "SELECT ctg.name, ctg.clone_id " . "FROM $source.contig ctg, $source.$table_name ci " . "WHERE ctg.clone_id = ci.clone_id " . "AND ctg.name not like 'ctg%' " . "AND ctg.name not like 'NA%'"; my $query_results1 = $dbh->selectall_arrayref($select_st1); my $i = 0; foreach my $contig_name (@$query_results1) { my $embl_acc = $contig_name->[0]; my $select_st2 = "SELECT sr.seq_region_id " . "FROM $target.seq_region sr " . "WHERE sr.name = '$embl_acc'"; my @query_results2 = $dbh->selectrow_array($select_st2); push @{$query_results1->[$i]},@query_results2; $i++; } foreach my $clone (@$query_results1) { my $seq_reg_id = $clone->[2]; my $clone_id = $clone->[1]; my $update_query = "UPDATE $target.$table_name " . "SET clone_id = '$seq_reg_id' " . "WHERE clone_id = '$clone_id'"; $dbh->do($update_query); } my $alter_struct_1 = "ALTER table $target.$table_name " . "CHANGE clone_id seq_region_id int(10) not null"; my $alter_struct_2 = "ALTER table $target.$table_name " . "add unique index (seq_region_id)"; $dbh->do($alter_struct_1); $dbh->do($alter_struct_2); } # assembly_tag
$self->debug("Vega Danio_specific - Transforming contig_id into seq_region_id for assembly_tag"); # first remove orphans from assembly_tag table (i.e. entries pointing to
# non-existing contigs)
my $numrows = $dbh->do(qq( DELETE at FROM $source.assembly_tag at LEFT JOIN $source.contig c ON c.contig_id = at.contig_id WHERE c.contig_id IS NULL )); $self->debug(" Deleted $numrows orphans from assembly_tag"); my $select_st3 = "SELECT ctg.name, ctg.contig_id " . "FROM $source.contig ctg, $source.assembly_tag at " . "WHERE ctg.contig_id = at.contig_id " . "AND ctg.name not like 'ctg%' " . "AND ctg.name not like 'NA%'"; my $query_results3 = $dbh->selectall_arrayref($select_st3); my $j = 0; foreach my $contig_name (@$query_results3) { my $embl_acc = $contig_name->[0]; my $select_st4 = "SELECT sr.seq_region_id " . "FROM $target.seq_region sr " . "WHERE sr.name = '$embl_acc'"; my @query_results4 = $dbh->selectrow_array($select_st4); push @{$query_results3->[$j]}, @query_results4; $j++; } foreach my $contig (@$query_results3) { my $seq_reg_id = $contig->[2]; my $contig_id = $contig->[1]; my $update_query = "UPDATE $target.assembly_tag " . "SET contig_id = '$seq_reg_id' " . "WHERE contig_id = '$contig_id'"; $dbh->do($update_query); } $dbh->do(" ALTER TABLE $target.assembly_tag CHANGE contig_id seq_region_id int(10) UNSIGNED NOT NULL"); $dbh->do(" ALTER TABLE $target.assembly_tag CHANGE contig_start seq_region_start int(10)"); $dbh->do(" ALTER TABLE $target.assembly_tag CHANGE contig_end seq_region_end int(10)"); $dbh->do(" ALTER TABLE $target.assembly_tag CHANGE contig_strand seq_region_strand tinyint(1)"); } 1;
}
General documentation
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