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Summary

Bio::DB::GFF::Adaptor::biofetch -- Cache BioFetch objects in a Bio::DB::GFF database

Package variables

Included modules

Inherit

Bio::DB::GFF::Adaptor::dbi::mysql

Synopsis

Proof of principle. Not for production use.

Description

This adaptor is a proof-of-principle. It is used to fetch BioFetch
sequences into a Bio::DB::GFF database (currently uses a hard-coded
EMBL database) as needed. This allows the Generic Genome Browser to
be used as a Genbank/EMBL browser.

Methods

Methods description

 Title   : new
 Usage   : $db = Bio::DB::GFF->new(-adaptor=>'biofetch',@args)
 Function: create a new adaptor
 Returns : a Bio::DB::GFF object
 Args    : see below
 Status  : Public

  Argument       Description
  --------       -----------

  -dsn           the DBI data source, e.g. 'dbi:mysql:ens0040'

  -user          username for authentication

  -pass          the password for authentication

  -proxy         [['http','ftp'],'http://proxy:8080']

Methods code

sub _load_embl {

  my $self = shift;
  my $acc  = shift;
  my $seq  = shift;
  my $refclass = $self->refclass;
  my $locus    = $seq->id;

  # begin loading
  $self->setup_load();

  # first synthesize the entry for the top-level feature
  my @aliases;
  foreach ($seq->accession,$seq->get_secondary_accessions) {
    next if lc($_) eq lc($acc);
    push @aliases,[Alias => $_];
  }
  $self->load_gff_line(
		       {
			ref    => $acc,
			class  => $refclass,
			source => 'EMBL',
			method => 'origin',
			start  => 1,
			stop   => $seq->length,
			score  => undef,
			strand => '.',
			phase  => '.',
			gclass => $self->refclass,
			gname  => $acc,
			tstart => undef,
			tstop  => undef,
			attributes  => [[Note => $seq->desc],@aliases],
		       }
		      );
  # now load each feature in turn
  for my $feat ($seq->all_SeqFeatures) {
    my $attributes = $self->get_attributes($feat);
    my $name       = $self->guess_name($attributes);

    my $location = $feat->location;
    my @segments = map {[$_->start,$_->end,$_->seq_id]}
      $location->can('sub_Location') ? $location->sub_Location : $location;

    my $type     =  $feat->primary_tag eq 'CDS' ? 'mRNA'  : $feat->primary_tag;
    my $parttype =  $feat->primary_tag eq 'gene' ? 'exon' : $feat->primary_tag;

    if ($feat->primary_tag =~ /^(gene|CDS)$/) {
      $self->load_gff_line( {
			     ref    => $acc,
			     class  => $refclass,
			     source => 'EMBL',
			     method => $type,
			     start  => $location->start,
			     stop   => $location->end,
			     score  => $feat->score || undef,
			     strand => $feat->strand > 0 ? '+' : ($feat->strand < 0 ? '-' : '.'),
			     phase  => $feat->frame || '.',
			     gclass => $name->[0],
			     gname  => $name->[1],
			     tstart => undef,
			     tstop  => undef,
			     attributes  => $attributes,
			    }
			  );
      @$attributes = ();
    }

    for my $segment (@segments) {

      $self->load_gff_line( {
			     ref    => $segment->[2] eq $locus ? $acc : $segment->[2],
			     class  => $refclass,
			     source => 'EMBL',
			     method => $parttype,
			     start  => $segment->[0],
			     stop   => $segment->[1],
			     score  => $feat->score || undef,
			     strand => $feat->strand > 0 ? '+' : ($feat->strand < 0 ? '-' : '.'),
			     phase  => $feat->frame || '.',
			     gclass => $name->[0],
			     gname  => $name->[1],
			     tstart => undef,
			     tstop  => undef,
			     attributes  => $attributes,
			    }
			  );
    }

  }

  # finish loading
  $self->finish_load();

  # now load the DNA
  $self->load_sequence_string($acc,$seq->seq);

  1;

}

sub get_attributes {

  my $self = shift;
  my $seq  = shift;

  my @tags = $seq->all_tags or return;
  my @result;
  foreach my $tag (@tags) {
    foreach my $value ($seq->each_tag_value($tag)) {
      push @result,[$tag=>$value];
    }
  }\@
  result;

}

sub guess_name {

  my $self = shift;
  my $attributes = shift;
  my @ordered_attributes = sort {($preferred_tags{$a->[0]} || 0) <=> ($preferred_tags{$b->[0]} || 0)} @$attributes;
  my $best = pop @ordered_attributes;
  @$attributes = @ordered_attributes;
  return $best;
}



1;

}

sub load_from_embl {

  my $self = shift;
  my $db   = shift;
  my $acc  = shift or $self->throw('Must provide an accession ID');

  my $biofetch;
  if ($self->{_biofetch}{$db}) {
    $biofetch = $self->{_biofetch}{$db};
  } else {
    $biofetch = $self->{_biofetch}{$db} = Bio::DB::BioFetch->new(-db=>$db);
    $biofetch->retrieval_type('tempfile');
    $biofetch->proxy(@{$self->{_proxy}}) if $self->{_proxy};
  }

  my $seq  = eval {$biofetch->get_Seq_by_id($acc)} or return;
  $self->_load_embl($acc,$seq);
  1;

}

sub load_from_file {

  my $self = shift;
  my $file = shift;

  my $format = $file =~ /\.(gb|genbank|gbk)$/i ? 'genbank' : 'embl';

  my $seqio = Bio::SeqIO->new( '-format' => $format, -file => $file);
  my $seq   = $seqio->next_seq;

  $self->_load_embl($seq->accession,$seq);
  1;

}

sub new {

  my $class = shift;
  my $self  = $class->SUPER::new(@_);
  my ($proxy) = rearrange(['PROXY'],@_);
  if ($proxy) {
    my @args = ref($proxy) ? @$proxy : eval $proxy;
    $self->{_proxy} =\@ args if @args;
  }
  $self;

}

sub refclass {

  my $self = shift;
  my $refname = shift;
  'Accession';

}

sub segment {

  my $self = shift;
  my @segments = $self->SUPER::segment(@_);

  if (!@segments) {
    my $refclass = $self->refclass;

    my %args = $self->setup_segment_args(@_);
    if ($args{-class} && $args{-class} =~ /$refclass/oi) {
      return unless $self->load_from_embl('embl'=>$args{-name});
      @segments = $self->SUPER::segment(@_);
    } elsif ($args{-class} && $args{-class} =~ /refseq|swall|embl/i) { #hack to get refseq names
      return unless $self->load_from_embl(lc($args{-class})=>$args{-name});
      $args{-class} = $self->refclass;
      @segments = $self->SUPER::segment(%args);
    }
  }

  $self->_multiple_return_args(@segments);
}

# default is to return 'Sequence' as the class of all references

}

General documentation

Lincoln Stein <lstein@cshl.org>.
Copyright 2002 Cold Spring Harbor Laboratory.
This library is free software; you can redistribute it and/or modify
it under the same terms as Perl itself.