Bio::Seq
PrimaryQual
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Summary
Bio::Seq::PrimaryQual - Bioperl lightweight Quality Object
Package variables
No package variables defined.
Included modules
Inherit
Synopsis
use Bio::Seq::PrimaryQual;
# you can use either a space-delimited string for quality
my $string_quals = "10 20 30 40 50 40 30 20 10";
my $qualobj = Bio::Seq::PrimaryQual->new
( '-qual' => $string_quals,
'-id' => 'QualityFragment-12',
'-accession_number' => 'X78121',
);
# _or_ you can use an array of quality values
my @q2 = split/ /,$string_quals;
$qualobj = Bio::Seq::PrimaryQual->new( '-qual' => \@q2,
'-primary_id' => 'chads primary_id',
'-desc' => 'chads desc',
'-accession_number' => 'chads accession_number',
'-id' => 'chads id'
);
# to get the quality values out:
my @quals = @{$qualobj->qual()};
# to give _new_ quality values
my $newqualstring = "50 90 1000 20 12 0 0";
$qualobj->qual($newqualstring);
Description
This module provides a mechanism for storing quality
values. Much more useful as part of
Bio::Seq::SeqWithQuality where these quality values
are associated with the sequence information.
Methods
Methods description
Title : accession_number()
Usage : $unique_biological_key = $obj->accession_number();
Function: Returns the unique biological id for a sequence, commonly
called the accession_number. For sequences from established
databases, the implementors should try to use the correct
accession number. Notice that primary_id() provides the unique id
for the implemetation, allowing multiple objects to have the same
accession number in a particular implementation. For sequences
with no accession number, this method should return "unknown".
Returns : A string
Args : None |
Title : desc()
Usage : $qual->desc($newval);
$description = $qual->desc();
Function: Get/set description text for a qual object
Example :
Returns : Value of desc
Args : newvalue (optional) |
Title : display_id()
Usage : $id_string = $obj->display_id();
Function: returns the display id, aka the common name of the Quality
object.
The semantics of this is that it is the most likely string to be
used as an identifier of the quality sequence, and likely to have
"human" readability. The id is equivalent to the ID field of the
GenBank/EMBL databanks and the id field of the Swissprot/sptrembl
database. In fasta format, the >(\S+) is presumed to be the id,
though some people overload the id to embed other information.
Bioperl does not use any embedded information in the ID field,
and people are encouraged to use other mechanisms (accession
field for example, or extending the sequence object) to solve
this. Notice that $seq->id() maps to this function, mainly for
legacy/convience issues
Returns : A string
Args : None |
Title : id()
Usage : $id = $qual->id();
Function: Return the ID of the quality. This should normally be (and
actually is in the implementation provided here) just a synonym
for display_id().
Returns : A string.
Args : None. |
Title : length()
Usage : $length = $qual->length();
Function: Return the length of the array holding the quality values.
Under most circumstances, this should match the number of quality
values but no validation is done when the PrimaryQual object is
constructed and non-digits could be put into this array. Is this
a bug? Just enough rope...
Returns : A scalar (the number of elements in the quality array).
Args : None. |
Title : new()
Usage : $qual = Bio::Seq::PrimaryQual->new
( -qual => '10 20 30 40 50 50 20 10',
-id => 'human_id',
-accession_number => 'AL000012',
);
Function: Returns a new Bio::Seq::PrimaryQual object from basic
constructors, being a string _or_ a reference to an array for the
sequence and strings for id and accession_number. Note that you
can provide an empty quality string.
Returns : a new Bio::Seq::PrimaryQual object |
Title : primary_id()
Usage : $unique_implementation_key = $obj->primary_id();
Function: Returns the unique id for this object in this implementation.
This allows implementations to manage their own object ids in a
way the implementaiton can control clients can expect one id to
map to one object. For sequences with no accession number, this
method should return a stringified memory location.
Returns : A string
Args : None |
Title : qual()
Usage : @quality_values = @{$obj->qual()};
Function: Returns the quality as a reference to an array containing the
quality values. The individual elements of the quality array are
not validated and can be any numeric value.
Returns : A reference to an array. |
Title : qualat($position)
Usage : $quality = $obj->qualat(10);
Function: Return the quality value at the given location, where the
first value is 1 and the number is inclusive, ie 1-2 are the first
two bases of the sequence. Start cannot be larger than end but can
be equal.
Returns : A scalar.
Args : A position. |
Title : subqual($start,$end)
Usage : @subset_of_quality_values = @{$obj->subqual(10,40)};
Function: returns the quality values from $start to $end, where the
first value is 1 and the number is inclusive, ie 1-2 are the
first two bases of the sequence. Start cannot be larger than
end but can be equal.
Returns : A reference to an array.
Args : a start position and an end position |
Title : to_string()
Usage : $quality = $obj->to_string();
Function: Return a textual representation of what the object contains.
For this module, this function will return:
qual
display_id
accession_number
primary_id
desc
id
length
Returns : A scalar.
Args : None. |
Title : validate_qual($qualstring)
Usage : print("Valid.") if { &validate_qual($self,$qualities); }
Function: Make sure that the quality, if it has length > 0, contains at
least one digit. Note that quality strings are parsed into arrays
using split/\d+/,$quality_string, so make sure that your quality
scalar looks like this if you want it to be parsed properly.
Returns : 1 for a valid sequence (WHY? Shouldn\'t it return 0? <boggle>)
Args : a scalar (any scalar, why PrimarySeq author?) and a scalar
containing the string to validate. |
Methods code
sub accession_number
{ my( $obj, $acc ) = @_;
if (defined $acc) {
$obj->{'accession_number'} = $acc;
} else {
$acc = $obj->{'accession_number'};
$acc = 'unknown' unless defined $acc;
}
return $acc;} | sub desc
{ my ($obj,$value) = @_;
if( defined $value) {
$obj->{'desc'} = $value;
}
return $obj->{'desc'};} | sub display_id
{ my ($obj,$value) = @_;
if( defined $value) {
$obj->{'display_id'} = $value;
}
return $obj->{'display_id'};} | sub id
{ my ($self,$value) = @_;
if( defined $value ) {
return $self->display_id($value);
}
return $self->display_id();} | sub length
{ my $self = shift;
if (ref($self->{qual}) ne "ARRAY") {
$self->warn("{qual} is not an array here. Why? It appears to be ".ref($self->{qual})."(".$self->{qual}."). Good thing this can _never_ happen.");
}
return scalar(@{$self->{qual}});} | sub new
{ my ($class, @args) = @_;
my $self = $class->SUPER::new(@args);
my($qual,$id,$acc,$pid,$desc,$given_id) =
$self->_rearrange([qw(QUAL
DISPLAY_ID
ACCESSION_NUMBER
PRIMARY_ID
DESC
ID
)],
@args);
if( defined $id && defined $given_id ) {
if( $id ne $given_id ) {
$self->throw("Provided both id and display_id constructor functions. [$id] [$given_id]");
}
}
if( defined $given_id ) { $id = $given_id; }
$self->qual($qual ? $qual : []);
$id && $self->display_id($id);
$acc && $self->accession_number($acc);
$pid && $self->primary_id($pid);
$desc && $self->desc($desc);
return $self;} | sub primary_id
{ my ($obj,$value) = @_;
if( defined $value) {
$obj->{'primary_id'} = $value;
}
return $obj->{'primary_id'};} | sub qual
{ my ($self,$value) = @_;
if( ! defined $value || length($value) == 0 ) {
$self->{'qual'} ||= [];
} elsif( ref($value) =~ /ARRAY/i ) {
$self->{'qual'} = $value;
} elsif(! $self->validate_qual($value)){
$self->throw("Attempting to set the quality to [$value] which does not look healthy");
} else {
$self->{'qual'} = [split(/\s+/,$value)];
}
return $self->{'qual'};} | sub qualat
{ my ($self,$val) = @_;
my @qualat = @{$self->subqual($val,$val)};
if (scalar(@qualat) == 1) {
return $qualat[0];
}
else {
$self->throw("AAAH! qualat provided more then one quality.");
}} | sub subqual
{ my ($self,$start,$end) = @_;
if( $start > $end ){
$self->throw("in subqual, start [$start] has to be greater than end [$end]");
}
if( $start <= 0 || $end > $self->length ) {
$self->throw("You have to have start positive and length less than the total length of sequence [$start:$end] Total ".$self->length."");
}
$start--;
$end--;
my @sub_qual_array = @{$self->{qual}}[$start..$end];
return\@ sub_qual_array;} | sub to_string
{ my ($self,$out,$result) = shift;
$out = "qual: ".join(',',@{$self->qual()});
foreach (qw(display_id accession_number primary_id desc id)) {
$result = $self->$_();
if (!$result) { $result = "<unset>"; }
$out .= "$_: $result\n";
}
return $out;} | sub to_string_automatic
{ my ($self,$sub_result,$out) = shift;
foreach (sort keys %$self) {
print("Working on $_\n");
eval { $self->$_(); };
if ($@) { $sub_result = ref($_); }
elsif (!($sub_result = $self->$_())) {
$sub_result = "<unset>";
}
if (ref($sub_result) eq "ARRAY") {
print("This thing ($_) is an array!\n");
$sub_result = join(',',@$sub_result);
}
$out .= "$_: ".$sub_result."\n";
}
return $out;
}
1;} | sub validate_qual
{
my ($self,$qualstr) = @_;
return 0 if (!defined $qualstr || CORE::length($qualstr) <= 0);
return 1 if( $qualstr =~ /\d/);
return 0; } | General documentation
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bio.perl.org/MailList.html - About the mailing lists
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via email
or the web:
bioperl-bugs@bio.perl.org
http://bugzilla.bioperl.org/
| AUTHOR - Chad Matsalla | Top |
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _