Bio::EnsEMBL::DBSQL DnaAlignFeatureAdaptor
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Summary
Bio::EnsEMBL::DBSQL::DnaAlignFeatureAdaptor - Adaptor for DnaAlignFeatures
Package variables
No package variables defined.
Included modules
Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor
Bio::EnsEMBL::DnaDnaAlignFeature
Bio::EnsEMBL::Utils::Exception qw ( throw warning )
Data::Dumper
Inherit
Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor
Synopsis
  $dafa = $registry->get_adaptor( 'Human', 'Core', 'DnaAlignFeature' );

  my @features = @{ $dafa->fetch_all_by_Slice($slice) };

  $dafa->store(@features);
Description
This is an adaptor responsible for the retrieval and storage of
DnaDnaAlignFeatures from the database. This adaptor inherits most of its
functionality from the BaseAlignFeatureAdaptor and BaseFeatureAdaptor
superclasses.
Methods
_columnsDescriptionCode
_left_join
No description
Code
_objs_from_sthDescriptionCode
_tablesDescriptionCode
list_dbIDsDescriptionCode
save
No description
Code
storeDescriptionCode
Methods description
_columnscode    nextTop
  Args       : none
  Example    : @columns = $self->_columns
  Description: PROTECTED implementation of abstract superclass method.  
               Returns a list of columns that are needed for object creation.
  Returntype : list of strings
  Exceptions : none
  Caller     : Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor::generic_fetch
  Status     : Stable
_objs_from_sthcodeprevnextTop
  Arg [1]    : DBI statement handle $sth
               an exectuted DBI statement handle generated by selecting 
               the columns specified by _columns() from the table specified 
               by _table()
  Example    : @dna_dna_align_feats = $self->_obj_from_hashref
  Description: PROTECTED implementation of superclass abstract method. 
               Creates DnaDnaAlignFeature objects from a DBI hashref
  Returntype : listref of Bio::EnsEMBL::DnaDnaAlignFeatures
  Exceptions : none
  Caller     : Bio::EnsEMBL::BaseFeatureAdaptor::generic_fetch
  Status     : Stable
_tablescodeprevnextTop
  Args       : none
  Example    : @tabs = $self->_tables
  Description: PROTECTED implementation of the abstract method inherited from
               BaseFeatureAdaptor.  Returns list of [tablename, alias] pairs
  Returntype : list of listrefs of strings
  Exceptions : none
  Caller     : Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor::generic_fetch
  Status     : Stable
list_dbIDscodeprevnextTop
  Arg [1]    : none
  Example    : @feature_ids = @{$dna_align_feature_adaptor->list_dbIDs()};
  Description: Gets an array of internal ids for all dna align features in 
               the current db
  Arg[1]     : <optional> int. not 0 for the ids to be sorted by the seq_region.
  Returntype : list of ints
  Exceptions : none
  Caller     : ?
  Status     : Stable
storecodeprevnextTop
  Arg [1]    : list of Bio::EnsEMBL::DnaAlignFeatures @feats
               the features to store in the database
  Example    : $dna_align_feature_adaptor->store(@features);
  Description: Stores a list of DnaAlignFeatures in the database
  Returntype : none
  Exceptions : throw if any of the provided features cannot be stored
               which may occur if:
                 * The feature does not have an associate Slice
                 * The feature does not have an associated analysis
                 * The Slice the feature is associated with is on a seq_region
                   unknown to this database
               A warning is given if:
                 * The feature has already been stored in this db
  Caller     : Pipeline
  Status     : Stable
Methods code
_columnsdescriptionprevnextTop
sub _columns {
  my $self = shift;

  #warning, implementation of _objs_from_sth method depends on order of list
  return qw(daf.dna_align_feature_id
            daf.seq_region_id
            daf.analysis_id
            daf.seq_region_start
            daf.seq_region_end
            daf.seq_region_strand
            daf.hit_start
            daf.hit_end
            daf.hit_name
            daf.hit_strand
            daf.cigar_line
            daf.evalue
            daf.perc_ident
            daf.score
            daf.external_db_id
            daf.hcoverage
	    daf.external_data
	    daf.pair_dna_align_feature_id
	    exdb.db_name
	    exdb.db_display_name);
}
_left_joindescriptionprevnextTop
sub _left_join {
    return (['external_db',"exdb.external_db_id = daf.external_db_id"]);
}
_objs_from_sthdescriptionprevnextTop
sub _objs_from_sth {
  my ($self, $sth, $mapper, $dest_slice) = @_;

  #
  # This code is ugly because an attempt has been made to remove as many
  # function calls as possible for speed purposes.  Thus many caches and
  # a fair bit of gymnastics is used.
  #

# in case of userdata we need the features on the dest_slice
# in case of get_all_supporting_features dest_slice is not provided .. 
  my $sa = $dest_slice ? $dest_slice->adaptor() : $self->db()->get_SliceAdaptor();
  my $aa = $self->db->get_AnalysisAdaptor();

  my @features;
  my %analysis_hash;
  my %slice_hash;
  my %sr_name_hash;
  my %sr_cs_hash;

  my($dna_align_feature_id, $seq_region_id, $analysis_id, $seq_region_start,
     $seq_region_end, $seq_region_strand, $hit_start, $hit_end, $hit_name,
     $hit_strand, $cigar_line, $evalue, $perc_ident, $score,
     $external_db_id, $hcoverage, $extra_data, $pair_dna_align_feature_id,
     $external_db_name, $external_display_db_name);

  $sth->bind_columns(\$
    dna_align_feature_id,\$ seq_region_id,\$ analysis_id,\$ seq_region_start,\$
    seq_region_end,\$ seq_region_strand,\$ hit_start,\$ hit_end,\$ hit_name,\$
    hit_strand,\$ cigar_line,\$ evalue,\$ perc_ident,\$ score,\$
    external_db_id,\$ hcoverage,\$ extra_data,\$ pair_dna_align_feature_id,\$
     external_db_name,\$ external_display_db_name);

  my $asm_cs;
  my $cmp_cs;
  my $asm_cs_vers;
  my $asm_cs_name;
  my $cmp_cs_vers;
  my $cmp_cs_name;
  if($mapper) {
    $asm_cs = $mapper->assembled_CoordSystem();
    $cmp_cs = $mapper->component_CoordSystem();
    $asm_cs_name = $asm_cs->name();
    $asm_cs_vers = $asm_cs->version();
    $cmp_cs_name = $cmp_cs->name();
    $cmp_cs_vers = $cmp_cs->version();
  }

  my $dest_slice_start;
  my $dest_slice_end;
  my $dest_slice_strand;
  my $dest_slice_length;
  my $dest_slice_sr_name;
  my $dest_slice_seq_region_id;

  if($dest_slice) {
    $dest_slice_start  = $dest_slice->start();
    $dest_slice_end    = $dest_slice->end();
    $dest_slice_strand = $dest_slice->strand();
    $dest_slice_length = $dest_slice->length();
    $dest_slice_sr_name = $dest_slice->seq_region_name();
    $dest_slice_seq_region_id = $dest_slice->get_seq_region_id();
  }

  FEATURE: while($sth->fetch()) {
    #get the analysis object
    my $analysis = $analysis_hash{$analysis_id} ||=
      $aa->fetch_by_dbID($analysis_id);

    #get the slice object
    my $slice = $slice_hash{"ID:".$seq_region_id};

    if(!$slice) {
      $slice = $sa->fetch_by_seq_region_id($seq_region_id);
      $slice_hash{"ID:".$seq_region_id} = $slice;
      $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
      $sr_cs_hash{$seq_region_id} = $slice->coord_system();
    }

    my $sr_name = $sr_name_hash{$seq_region_id};
    my $sr_cs   = $sr_cs_hash{$seq_region_id};
    #
    # remap the feature coordinates to another coord system
    # if a mapper was provided
    #
    if($mapper) {

      ($seq_region_id,$seq_region_start,$seq_region_end,$seq_region_strand) =
        $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
                          $seq_region_strand, $sr_cs);

      #skip features that map to gaps or coord system boundaries
      next FEATURE if(!defined($seq_region_id));

      #get a slice in the coord system we just mapped to
      if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
        $slice = $slice_hash{"ID:".$seq_region_id} ||=
          $sa->fetch_by_seq_region_id($seq_region_id);
      } else {
        $slice = $slice_hash{"ID:".$seq_region_id} ||=
          $sa->fetch_by_seq_region_id($seq_region_id);
      }
    }

    #
    # If a destination slice was provided convert the coords
    # If the dest_slice starts at 1 and is foward strand, nothing needs doing
    #
    if($dest_slice) {
      if($dest_slice_start != 1 || $dest_slice_strand != 1) {
        if($dest_slice_strand == 1) {
          $seq_region_start = $seq_region_start - $dest_slice_start + 1;
          $seq_region_end   = $seq_region_end   - $dest_slice_start + 1;
        } else {
          my $tmp_seq_region_start = $seq_region_start;
          $seq_region_start = $dest_slice_end - $seq_region_end + 1;
          $seq_region_end   = $dest_slice_end - $tmp_seq_region_start + 1;
          $seq_region_strand *= -1;
        }

        #throw away features off the end of the requested slice
        if($seq_region_end < 1 || $seq_region_start > $dest_slice_length ||
	  ( $dest_slice_seq_region_id ne $seq_region_id ))  {
          next FEATURE;
        }
      }
      $slice = $dest_slice;
    }

    # Finally, create the new DnaAlignFeature.
    push( @features,
          $self->_create_feature_fast(
                                  'Bio::EnsEMBL::DnaDnaAlignFeature', {
                                    'slice'      => $slice,
                                    'start'      => $seq_region_start,
                                    'end'        => $seq_region_end,
                                    'strand'     => $seq_region_strand,
                                    'hseqname'   => $hit_name,
                                    'hstart'     => $hit_start,
                                    'hend'       => $hit_end,
                                    'hstrand'    => $hit_strand,
                                    'score'      => $score,
                                    'p_value'    => $evalue,
                                    'percent_id' => $perc_ident,
                                    'cigar_string' => $cigar_line,
                                    'analysis'     => $analysis,
                                    'adaptor'      => $self,
                                    'dbID' => $dna_align_feature_id,
                                    'external_db_id' => $external_db_id,
                                    'hcoverage'      => $hcoverage,
				    'extra_data'     => $extra_data ? $self->get_dumped_data($extra_data) : '',
				    'dbname'         => $external_db_name,
				    'db_display_name' => $external_display_db_name,
				    'pair_dna_align_feature_id' => $pair_dna_align_feature_id
                                  } ) );

  }

  return\@ features;
}
_tablesdescriptionprevnextTop
sub _tables {
  my $self = shift;

  return (['dna_align_feature', 'daf'],['external_db','exdb']);
}
list_dbIDsdescriptionprevnextTop
sub list_dbIDs {
   my ($self, $ordered) = @_;

   return $self->_list_dbIDs("dna_align_feature",undef, $ordered);
}

1;
}
savedescriptionprevnextTop
sub save {
  my ($self, $features) = @_;

  my @feats = @$features;
  throw("Must call store with features") if( scalar(@feats) == 0 );

  my @tabs = $self->_tables;
  my ($tablename) = @{$tabs[0]};

  my $db = $self->db();
  my $analysis_adaptor = $db->get_AnalysisAdaptor();

  my $sql = qq{INSERT INTO $tablename (seq_region_id, seq_region_start, seq_region_end, seq_region_strand, hit_start, hit_end, hit_strand, hit_name, cigar_line, analysis_id, score, evalue, perc_ident, external_db_id, hcoverage, pair_dna_align_feature_id, external_data) VALUES (?, ?, ?, ?, ?, ?, ?, ?, ?, ?, ?, ?, ?, ?, ?, ?, ?)};

  my %analyses = ();

  my $sth = $self->prepare($sql);
     
 FEATURE: foreach my $feat ( @feats ) {
    if( !ref $feat || !$feat->isa("Bio::EnsEMBL::DnaDnaAlignFeature") ) {
      throw("feature must be a Bio::EnsEMBL::DnaDnaAlignFeature,"
            . " not a [".ref($feat)."].");
    }

    if($feat->is_stored($db)) {
      warning("DnaDnaAlignFeature [".$feat->dbID."] is already stored" .
              " in this database.");
      next FEATURE;
    }

    my $hstart  = $feat->hstart || 0; # defined $feat->hstart  ? $feat->hstart : $feat->start ;
    my $hend    = $feat->hend   || 0; # defined $feat->hend    ? $feat->hend : $feat->end;
    my $hstrand = $feat->hstrand|| 0; # defined $feat->hstrand ? $feat->hstrand : $feat->strand;
    if( $hstart && $hend ) {
      if($hend < $hstart) {
        throw("Invalid Feature start/end [$hstart/$hend]. Start must be less than or equal to end.");
      }
    }
    my $cigar_string = $feat->cigar_string();
    if(!$cigar_string) {
      $cigar_string = $feat->length() . 'M';
      warning("DnaDnaAlignFeature does not define a cigar_string.\n" .
              "Assuming ungapped block with cigar_line=$cigar_string .");
    }

    my $hseqname = $feat->hseqname();
    if(!$hseqname) {
      throw("DnaDnaAlignFeature must define an hseqname.");
    }

    if(!defined($feat->analysis)) {
      throw("An analysis must be attached to the features to be stored.");
    }

    #store the analysis if it has not been stored yet
    if(!$feat->analysis->is_stored($db)) {
      $analysis_adaptor->store($feat->analysis());
    }

    $analyses{ $feat->analysis->dbID }++;

    my $original = $feat;
    my $seq_region_id;
    ($feat, $seq_region_id) = $self->_pre_store_userdata($feat);

    my $extra_data = $feat->extra_data ? $self->dump_data($feat->extra_data) : '';

    $sth->bind_param(1,$seq_region_id,SQL_INTEGER);
    $sth->bind_param(2,$feat->start,SQL_INTEGER);
    $sth->bind_param(3,$feat->end,SQL_INTEGER);
    $sth->bind_param(4,$feat->strand,SQL_TINYINT);
    $sth->bind_param(5,$hstart,SQL_INTEGER);
    $sth->bind_param(6,$hend,SQL_INTEGER);
    $sth->bind_param(7,$hstrand,SQL_TINYINT);
    $sth->bind_param(8,$hseqname,SQL_VARCHAR);
    $sth->bind_param(9,$cigar_string,SQL_LONGVARCHAR);
    $sth->bind_param(10,$feat->analysis->dbID,SQL_INTEGER);
    $sth->bind_param(11,$feat->score,SQL_DOUBLE);
#    $sth->bind_param(11,$feat->score); # if the above statement does not work it means you need to upgrade DBD::mysql, meantime you can replace it with this line
    $sth->bind_param(12,$feat->p_value,SQL_DOUBLE);
    $sth->bind_param(13,$feat->percent_id,SQL_FLOAT);
    $sth->bind_param(14,$feat->external_db_id,SQL_INTEGER);
    $sth->bind_param(15,$feat->hcoverage,SQL_DOUBLE);
    $sth->bind_param(16,$feat->pair_dna_align_feature_id,SQL_INTEGER);
    $sth->bind_param(17,$extra_data,SQL_LONGVARCHAR);


    $sth->execute();
    $original->dbID($sth->{'mysql_insertid'});
    $original->adaptor($self);
  }

  $sth->finish();

## js5 hack to update meta_coord table... 
  if( keys %analyses ) {

    my $sth = $self->prepare( 'select sr.coord_system_id, max(daf.seq_region_end-daf.seq_region_start) from seq_region as sr, dna_align_feature as daf where daf.seq_region_id=sr.seq_region_id and analysis_id in ('.join(',',keys %analyses).') group by coord_system_id' );
    $sth->execute;

    foreach( @{ $sth->fetchall_arrayref } ) {
      my $sth2 = $self->prepare( qq(insert ignore into meta_coord values("dna_align_feature",$_->[0],$_->[1])) );
      $sth2->execute;
      $sth2->finish;

      $sth2 = $self->prepare( qq(update meta_coord set max_length = $_->[1] where coord_system_id = $_->[0] and table_name="dna_align_feature" and max_length < $_->[1]) );
      $sth2->execute;
      $sth2->finish;
    }

    $sth->finish;
  }
}
storedescriptionprevnextTop
sub store {
  my ($self, @feats) = @_;

  throw("Must call store with features") if( scalar(@feats) == 0 );

  my @tabs = $self->_tables;
  my ($tablename) = @{$tabs[0]};

  my $db = $self->db();
  my $analysis_adaptor = $db->get_AnalysisAdaptor();

  my $sth = $self->prepare(
     "INSERT INTO $tablename (seq_region_id, seq_region_start, seq_region_end,
                             seq_region_strand, hit_start, hit_end,
                             hit_strand, hit_name, cigar_line,
                             analysis_id, score, evalue, perc_ident, external_db_id, 
                             hcoverage, pair_dna_align_feature_id)
     VALUES (?,?,?,?,?,?,?,?,?,?,?, ?, ?, ?, ?, ?)");

 FEATURE: foreach my $feat ( @feats ) {
    if( !ref $feat || !$feat->isa("Bio::EnsEMBL::DnaDnaAlignFeature") ) {
      throw("feature must be a Bio::EnsEMBL::DnaDnaAlignFeature,"
            . " not a [".ref($feat)."].");
    }

    if($feat->is_stored($db)) {
      warning("DnaDnaAlignFeature [".$feat->dbID."] is already stored" .
              " in this database.");
      next FEATURE;
    }

    my $hstart = $feat->hstart();
    my $hend   = $feat->hend();
    my $hstrand = $feat->hstrand();
    $self->_check_start_end_strand($hstart,$hend, $hstrand);

    my $cigar_string = $feat->cigar_string();
    if(!$cigar_string) {
      $cigar_string = $feat->length() . 'M';
      warning("DnaDnaAlignFeature does not define a cigar_string.\n" .
              "Assuming ungapped block with cigar_line=$cigar_string .");
    }

    my $hseqname = $feat->hseqname();
    if(!$hseqname) {
      throw("DnaDnaAlignFeature must define an hseqname.");
    }

    if(!defined($feat->analysis)) {
      throw("An analysis must be attached to the features to be stored.");
    }

    #store the analysis if it has not been stored yet
    if(!$feat->analysis->is_stored($db)) {
      $analysis_adaptor->store($feat->analysis());
    }

    my $original = $feat;
    my $seq_region_id;
    ($feat, $seq_region_id) = $self->_pre_store($feat);
    $sth->bind_param(1,$seq_region_id,SQL_INTEGER);
    $sth->bind_param(2,$feat->start,SQL_INTEGER);
    $sth->bind_param(3,$feat->end,SQL_INTEGER);
    $sth->bind_param(4,$feat->strand,SQL_TINYINT);
    $sth->bind_param(5,$hstart,SQL_INTEGER);
    $sth->bind_param(6,$hend,SQL_INTEGER);
    $sth->bind_param(7,$hstrand,SQL_TINYINT);
    $sth->bind_param(8,$hseqname,SQL_VARCHAR);
    $sth->bind_param(9,$cigar_string,SQL_LONGVARCHAR);
    $sth->bind_param(10,$feat->analysis->dbID,SQL_INTEGER);
    $sth->bind_param(11,$feat->score,SQL_DOUBLE);
    $sth->bind_param(12,$feat->p_value,SQL_DOUBLE);
    $sth->bind_param(13,$feat->percent_id,SQL_FLOAT);
    $sth->bind_param(14,$feat->external_db_id,SQL_INTEGER);
    $sth->bind_param(15,$feat->hcoverage,SQL_DOUBLE);
    $sth->bind_param(16,$feat->pair_dna_align_feature_id, SQL_INTEGER);

    $sth->execute();
    $original->dbID($sth->{'mysql_insertid'});
    $original->adaptor($self);
  }

  $sth->finish();
}
General documentation
LICENSETop
  Copyright (c) 1999-2009 The European Bioinformatics Institute and
  Genome Research Limited.  All rights reserved.

  This software is distributed under a modified Apache license.
  For license details, please see

    /info/about/code_licence.html
CONTACTTop
  Please email comments or questions to the public Ensembl
  developers list at <ensembl-dev@ebi.ac.uk>.

  Questions may also be sent to the Ensembl help desk at
  <helpdesk@ensembl.org>.